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A kind of arsenic-contaminated soil remediation fixative based on microbial assembly synthesis and its preparation and application method

A soil remediation and arsenic pollution technology, applied in the restoration of polluted soil, chemical instruments and methods, applications, etc., can solve the problems of disturbing the soil ecosystem, material loss, affecting soil structure and properties, etc. The effect of specific surface area

Active Publication Date: 2019-04-26
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once the nanoscale iron oxide powder is dispersed in large-scale use, it is easy to flow away with water, which will lead to material loss and increase costs, and will disturb the soil ecosystem due to the addition of a large amount of exogenous iron, affecting the structural properties of the soil

Method used

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  • A kind of arsenic-contaminated soil remediation fixative based on microbial assembly synthesis and its preparation and application method
  • A kind of arsenic-contaminated soil remediation fixative based on microbial assembly synthesis and its preparation and application method
  • A kind of arsenic-contaminated soil remediation fixative based on microbial assembly synthesis and its preparation and application method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Prepare the fixative for repairing arsenic-contaminated soil of the present invention:

[0038] (1) Aspergillus niger (Aspergillus niger) was activated and expanded by PDA medium, which was purchased from China Center for Type Culture Collection, strain number CCTCC AF 91006;

[0039] (2) 1.0g of α-Fe 2 o 3 Nanoparticles (particle size99.5%) were added to 100mL of liquid PDA medium, and ultrasonically dispersed with a power of 100W for 0.5h;

[0040] (3) will contain nano-α-Fe 2 o 3 Put the PDA culture medium into a high-temperature and high-pressure sterilizer, set at 115°C, and sterilize for 25 minutes. After high-temperature and high-pressure sterilization, the culture medium was transferred to an ultra-clean aseptic operating table, and sterilized with an ultraviolet lamp for 30 minutes;

[0041] (4) In the ultra-clean aseptic operation bench, inoculate the activated mycelium in step (1) into the sterilized medium of step (3), the inoculum size is 1 mL; the mas...

Embodiment 2

[0045] Prepare the fixative for repairing arsenic-contaminated soil of the present invention:

[0046] (1) The improved LB medium (per 100mL medium: LB medium 2.5g, glucose 9g, sodium nitrate 0.3g) was used to activate and expand the culture of Penicillium Chrysogenum F1. The strain was collected in Zhuzhou City, Hunan Province , preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, preservation number: CGMCC No.5599, and has applied for a patent;

[0047] (2) 1.0g of α-Fe 2 o 3 Nanoparticles (particle size99.5%) were added to 200mL liquid modified LB medium, and ultrasonically dispersed with a power of 100W for 1.5h;

[0048] (3) will contain nano-α-Fe 2 o 3 The modified LB medium was put into a high temperature and high pressure sterilizer, set at 115°C, and sterilized for 25 minutes. After high-temperature and high-pressure sterilization, the culture medium was transferred to an ultra-clean aseptic operating tab...

Embodiment 3

[0053] The soil was taken from a realgar mine waste dump. After being air-dried, impurity-removed and ground, it was passed through a 18-mesh nylon sieve. The soil pH value was 7.73, and the water-soluble and available As in the soil were 33.2mg / kg and 288.2mg / kg.

[0054] Respectively the fixing agent of embodiment 1 and 2 and pure nano-α-Fe 2 o 3 (Nano α-Fe is included in the fixative 2 o 3 Quantity and weighed pure nano-α-Fe 2 o 3 equal amounts, both 0.2g) into 5.0g of arsenic-contaminated soil, add deionized water according to the water-to-soil mass ratio of 2:1, and stir until uniform. After fixing for one week, extract the water-soluble arsenic in the soil with 50 mL of deionized water; use 50 mL of 0.5 mol / L NaHCO 3 Extract the available arsenic in the soil. After testing, the arsenic-contaminated soil was treated with the fixative of Examples 1 and 2 or pure nano-α-Fe 2 o 3 The contents of water-soluble As and available As in the soil before and after treatmen...

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Abstract

The invention discloses a microbe-assembly-synthesis-based arsenic polluted soil restoring and fixing agent, and preparation and application methods thereof. Many iron-base materials for arsenic polluted soil restoration at present influence the structural properties of the soil due to the introduction of abundant exogenous iron, and the iron salt fixing agent can easily acidify the soil after being applied, thereby restricting the development of the soil restoring and fixing agent in the aspect of soil restoration. In order to solve the problems above, nano alpha-Fe2O3 is used as a central substance and coated by mycelial fungus hypha to prepare the restoring and fixing agent for arsenic polluted soil. When the synthesized product is proportionally and uniformly mixed with soil, after seven days, the fixing rates for water-soluble arsenic and effective-state arsenic in the arsenic polluted soil respectively reach 83.2% and 96.4%. The fixing agent has the advantages of simple preparation process, high efficiency, no toxicity or harm and no secondary pollution, is biodegradable, can not destroy the physicochemical properties of the soil, and thus, is an environment-friendly fixing agent.

Description

technical field [0001] The invention relates to the technical field of combination of micro-nano material assembly and arsenic-contaminated soil remediation, in particular to a fixative product suitable for arsenic-contaminated soil remediation and its preparation and application method. Background technique [0002] Arsenic is widely used in many industries, and a large amount of arsenic remains in the soil or enters the atmosphere and water through the processes of mining, processing, use, and disposal, resulting in widespread soil As pollution. Arsenic is a highly toxic nonmetal. It mainly exists in the inorganic form of arsenite (As(III)) and arsenate (As(V)) in the natural environment and is transformed into each other through oxidation and reduction by microorganisms. Soil arsenic pollution will directly lead to a decline in the quality of agricultural products, and it will be enriched in humans and animals through the food chain, posing a serious threat to human healt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K17/14B09C1/10
CPCB09C1/105C09K17/14
Inventor 杨卫春粱丽芬柴立元杨志辉侍维王海鹰唐崇俭
Owner CENT SOUTH UNIV