Salacia compositions, methods of treatment by their administration, and methods of their preparation

A composition and technology of Pentaphyllum, applied in the directions of drug combinations, steroids, medical preparations containing active ingredients, etc., can solve the problem of lack of inhibitory effect of Pentaphyllum.

Inactive Publication Date: 2016-07-06
OMNIACTIVE HEALTH TECH CANADA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no studies on the inhibitory effects of Salacia alone on appetite or HMG-CoA reductase

Method used

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  • Salacia compositions, methods of treatment by their administration, and methods of their preparation
  • Salacia compositions, methods of treatment by their administration, and methods of their preparation
  • Salacia compositions, methods of treatment by their administration, and methods of their preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1: Preparation of five layer dragon composition

[0076] The reticulated five-layered dragon root is dried, ground, and extracted with 5 times the volume of ethanol by stirring at 60-65°C for 3 hours. The solvent was then filtered under vacuum and the filtrate was kept for later use. The material was extracted with 3 volumes of ethanol by stirring at 60-65°C for 1 hour. The solvent was filtered, the filtrates were combined, and then evaporated in a rotary evaporator at 50°C to obtain a dry red-brown powdery extract.

[0077] The results here are based on the composition obtained in Example 1, and represent an embodiment of the five layer dragon composition of the present invention. Based on the weight of the composition, the composition described in Example 1 consists of at least 12% of polyphenols, 2% of mangiferin, and 1% of 25,26-epoxy unchain terpene-1,3-dione composition.

[0078] In some embodiments, based on the weight of the composition, the composition of ...

Embodiment 2

[0080] Example 2A: Separation and characterization of the five layer dragon composition

[0081] UV determination of total polyphenol content (in gallic acid equivalent)

[0082] The five-layer dragon composition was dispersed in methanol at the required concentration, 1.0 ml of each sample was taken into a test tube, and combined with 5 ml of 10-fold diluted Folin-Ciocalteu reagent and 4 ml of 7.5 % Sodium carbonate mixed. The test tube was covered with parafilm and left to stand at room temperature for 50 minutes. Then read the absorbance at 765nm spectroscopy, with gallic acid as the standard.

[0083] Determination of mangiferin and its derivatives:

[0084] The mangiferin and its derivatives of the Wushelong composition were determined by HPLC. The column used was a MerckLiChrospher100RP18 column, 250×4mm, 5μm. The flow rate is 1.0 mL per minute, and the detector wavelength is 260 nm. Use 0.05% orthophosphoric acid (OPA) as mobile phase A and methanol:ACN (methanol:acetonitr...

Embodiment 3

[0091] Example 3: In vitro evaluation of five layer dragon composition by enzymatic analysis

[0092] The effect of the five layer dragon composition on HMG-CoA reductase was evaluated in vitro to evaluate its application for maintaining healthy lipid distribution. The effects on enzymes such as hormone-sensitive lipase and pancrelipase were further evaluated.

[0093] a. HMG-CoA reductase assay

[0094] Method: HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis. The activity of HMG-CoA reductase was evaluated with the HMG-CoA reductase assay kit (Sigma CS1090). The kit measures the oxidation of NADPH (nicotinamide adenine dinucleotide phosphate) caused by the catalytic subunit of HMG reductase in the presence of the substrate: HMG-CoA at an absorbance of 340 nm.

[0095] Results: The five layer dragon composition, as in Example 1, inhibited HMG-COA at a concentration of 1 mg / ml, IC50: 401.3 μg / ml (Table 1).

[0096] Table 1: The effect of the composition of Wush...

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Abstract

A Salacia composition described herein significantly reduces appetite, activates Hormone Sensitive Lipase, and manages a healthy lipid profile through inhibition of HMG-CoA Reductase enzyme. Salacia compositions described herein include of at least 12% of polyphenols, 2% of mangiferin and 1% of 25, 26-oxidofriedelane-l, 3-dione by weight of the composition in the form of extract. The composition is obtained by continuous solvent extraction, employing non-aqueous food grade solvents. Salacia compositions herein reduce appetite by about 18% of routine diet, when effective amounts are administered to a subject in need thereof. Effective management of a healthy lipid profile is thus possible with synergistic combination of reduction in energy intake, activation of Hormone Sensitive Lipase, and inhibition of HMG-CoA Reductase enzyme responsible for cholesterol metabolism.

Description

Invention field [0001] Describes the composition of Salacia, its preparation method, and the method of using them for treatment, as well as it significantly reduces appetite, activates hormone-sensitive lipase, and controls healthy lipid distribution by inhibiting HMG-CoA reductase. The five layer dragon composition described herein includes at least 12% of polyphenols, 2% of mangiferin and 1% of 25,26-oxidofriedelane in the form of extract based on the weight of the composition. 1,3-Diketone. The composition is prepared by using a non-aqueous food-grade solvent, and when administered in an effective amount to an individual in need, appetite is significantly reduced. The five layer dragon composition controls healthy lipid distribution by inhibiting HMG-CoA reductase and activating hormone-sensitive lipase. The novel five layer dragon composition of the present invention is safe for human use, is prepared by an economical method using conventional equipment, and can be used to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/37A61K31/352A61K31/575A61P3/04C07H7/04C07J71/00C12N9/04C12N9/16
CPCA23L33/10A23L33/105A61K31/122A61K31/7048A61K36/37A61P3/04A61P43/00A61K31/192
Inventor J·德什潘德K·伽南S·尤尔特V·朱图如
Owner OMNIACTIVE HEALTH TECH CANADA
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