A kind of seed culture medium and cultivation method utilizing Streptomyces orientalis to ferment and produce vancomycin

Abstract

The invention relates to a seed culture medium for producing vancomycin through fermentation of streptomyces orientalis and a cultivation method. By the adding of composite modified starches, fibroin powder, casein and other substances into the seed culture medium, the seed culture quality and the fermentation unit of vancomycin can be improved favorably, and the fermentation cost can be reduced; furthermore, the influence of the environment on original auxiliary material sources can be reduced to the maximum extent, so that sufficient supply of the original auxiliary material sources can be guaranteed, and efficient production of vancomycin is realized.

Description

technical field [0001] The invention belongs to the field of fermentation technology, in particular to a seed culture medium and a culture method of Streptomyces orientalis. Background technique [0002] Vancomycin is a glycopeptide antibiotic isolated from the fermentation broth of Streptomyces orientalis and has anti-Gram-positive bacteria. It was later developed by Lilly Company of the United States, and was approved by the U.S. Food and Drug Administration (FDA) for marketing in 1958. At present, it is the drug of choice for the treatment of severe infectious diseases caused by MRSA clinically. Therefore, it is praised by international antibiotic experts as "the last line of defense for human beings against stubborn drug-resistant strains" and "the ace antibiotic". [0003] At present, the fermentation production of vancomycin in China adopts a three-stage fermentation mode, and the main problems are as follows: [0004] 1 Glucose was added to the seed medium and ferme...

AI Technical Summary

Problems solved by technology

At the end of secondary seed cultivation, the concentration of bacteria was low, no more than 25%, which affected the technical level of vancomycin fermentation (fermentation tank 100m 3 above scale), resulting in low fermentation units, generally at 7000u / ml

[0006] 3 The fermentation period is long, generally exceeding 160h

In particular, the cultivation period of first-class seeds and second-class seeds is longer, exceeding 24 hours, resulting in higher energy consumption and affecting production costs

[0007] 4 Relevant literatures at home and abroad lack content on supplementation in vancomycin fermentation production process

[0008] 5 The production cost of vancomycin is relatively high, which reduces the market competitiveness of the product