Seed culture medium for producing vancomycin through fermentation of streptomyces orientalis and cultivation method

A culture method and vancomycin technology, applied in the field of fermentation, can solve the problems of long fermentation period, lack of vancomycin feed content, and high vancomycin production cost, and achieve the effect of increasing fermentation units

Active Publication Date: 2016-07-13
HEILONGJIANG LIANSHUN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the end of secondary seed cultivation, the concentration of bacteria was low, no more than 25%, which affected the technical level of vancomycin fermentation (fermentation tank 100m 3 above scale), resulting in low fermentation units, generally at 7000u / ml
[0006] 3 The fermentation period is long, generally exceeding 160h
In particular, the cultivation period of first-class seeds and second-class seeds is longer, exceeding 24 hours, resulting in higher energy consumption and affecting production costs
[0007] 4 Relevant literatures at home and abroad lack the content of feeding materials in the fermentation production process of vancomycin
[0008] 5. The production cost of vancomycin is relatively high, which reduces the market competitiveness of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] primary seed culture : The effective volume of the seed medium is 1m 3 .

[0045] Seed medium: 8kg of oxidized cross-linked starch, 13kg of maltodextrin, 7kg of fish meal, 4kg of silk fibroin powder, 10L of corn steep liquor, 2kg of biological nitrogen, 0.5kg of ammonium sulfate, 1kg of light calcium carbonate, and 0.01kg of glucoamylase.

[0046] First, sterilize the primary seed medium and cool it to 25°C, and keep the pressure with sterile air. The quality requirements of the sterilized primary seed medium are: amino nitrogen 41mg / 100ml, dissolved phosphorus 40ug / ml, total sugar 20mg / 100ml, pH6.1. Then, under the protection of the flame, the cultured oriental streptomycin mother bottle fermentation broth was inserted into the primary seed medium according to the inoculum amount of 0.1L for seed cultivation. The primary seed cultivation conditions were: tank pressure 0.03-0.05MPa ;Tank temperature 29~30℃; Air flow 20m 3 / h; stirring speed 60r / min. Transplant unt...

Embodiment 2

[0056] primary seed culture : The effective volume of the seed medium is 1m 3 .

[0057] Seed medium: 9kg of cross-linked esterified starch, 14kg of maltodextrin, 8kg of fish meal, 5kg of silk fibroin powder, 11L of corn steep liquor, 3kg of biological nitrogen, 0.6kg of ammonium sulfate, 2kg of light calcium carbonate, and 0.02kg of glucoamylase.

[0058] First, sterilize the primary seed medium and cool it to 26°C, and keep the pressure with sterile air. The quality requirements of the primary seed medium after sterilization are: amino nitrogen 43mg / 100ml, dissolved phosphorus 45ug / ml, total sugar 22mg / 100ml, pH6.3. Then, under the protection of the flame, the cultured oriental streptomycin mother bottle fermentation liquid was inserted into the primary seed medium according to the inoculum amount of 0.12L for seed cultivation. The primary seed cultivation conditions were: tank pressure 0.03-0.05MPa ;Tank temperature 29~30℃; Air flow 25m 3 / h; stirring speed 65r / min. ...

Embodiment 3

[0068] primary seed culture : The effective volume of the seed medium is 1m 3 .

[0069] Seed medium: oxidized cross-linked starch 10kg, maltodextrin 15kg, fish meal 9kg, silk fibroin powder 6kg, corn steep liquor 12L, biological nitrogen 4kg, ammonium sulfate 0.7kg, light calcium carbonate 3kg, glucoamylase 0.03kg.

[0070] First, sterilize the primary seed medium and cool it down to 27°C, and keep the pressure with sterile air. The quality requirements of the primary seed medium after sterilization are: amino nitrogen 45mg / 100ml, dissolved phosphorus 55ug / ml, total sugar 25mg / 100ml, pH6.5. Then, under the protection of the flame, the cultured oriental streptomycin mother bottle fermentation broth was inserted into the primary seed medium according to the inoculum amount of 0.15L for seed cultivation. The primary seed cultivation conditions were: tank pressure 0.03-0.05MPa ;Tank temperature 29~30℃; Air flow 30m 3 / h; stirring speed 70r / min. Transplant until the cell co...

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PUM

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Abstract

The invention relates to a seed culture medium for producing vancomycin through fermentation of streptomyces orientalis and a cultivation method. By the adding of composite modified starches, fibroin powder, casein and other substances into the seed culture medium, the seed culture quality and the fermentation unit of vancomycin can be improved favorably, and the fermentation cost can be reduced; furthermore, the influence of the environment on original auxiliary material sources can be reduced to the maximum extent, so that sufficient supply of the original auxiliary material sources can be guaranteed, and efficient production of vancomycin is realized.

Description

technical field [0001] The invention belongs to the field of fermentation technology, in particular to a seed culture medium and a culture method of Streptomyces orientalis. Background technique [0002] Vancomycin is a glycopeptide antibiotic isolated from the fermentation broth of Streptomyces orientalis and has anti-Gram-positive bacteria. It was later developed by Lilly Company of the United States, and was approved by the U.S. Food and Drug Administration (FDA) for marketing in 1958. At present, it is the drug of choice for the treatment of severe infectious diseases caused by MRSA clinically. Therefore, it is praised by international antibiotic experts as "the last line of defense for human beings against stubborn drug-resistant strains" and "the ace antibiotic". [0003] At present, the fermentation production of vancomycin in China adopts a three-stage fermentation mode, and the main problems are as follows: [0004] 1 Glucose was added to the seed medium and ferme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P21/02C12R1/465
Inventor 任勇
Owner HEILONGJIANG LIANSHUN BIOTECHNOLOGY CO LTD
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