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A kind of tissue culture method of vesicular grass

A technology of tissue culture and vesicle grass, which is applied in the field of plant tissue culture to achieve the effects of strict requirements, low pollution rate and cost reduction

Inactive Publication Date: 2018-04-10
INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the successful tissue culture of Physiophthora vesicosa, and it is necessary to screen the appropriate culture medium of Physicum vesicularis in actual production

Method used

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  • A kind of tissue culture method of vesicular grass
  • A kind of tissue culture method of vesicular grass
  • A kind of tissue culture method of vesicular grass

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The tissue culture method of vesicular grass, the method comprises the following steps:

[0030] 1.1 Establishment of explants

[0031] The test materials were vesicular grass plants collected by the Grass Industry Center of the Inner Mongolia Academy of Agriculture and Animal Husbandry in Abaga Banner, Xilingol League, Inner Mongolia Autonomous Region. They were planted in seedling pots, and the leaves on the current and robust branches were selected as explants.

[0032] 1.2 Test method

[0033] 1.2.1 Sterilization of explants

[0034] Select the leaves on the current year and strong branches as explants, cut them into 5×5mm squares with a scalpel, wash the explant squares with neutral detergent for 10min to remove the surface stains, rinse with tap water for 20min, and dry with filter paper After hydration, it was placed on an ultra-clean workbench for sterilization, and sterilized with 0.1% mercuric chloride. The treatment time is shown in Table 1. The sterile wat...

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Abstract

The invention discloses a tissue culture method for physochlaina. The tissue culture method comprises the following steps: sterilizing an explant; performing primary induction culture on physochlaina buds; performing subculture multiplication culture; rooting tissue culture seedlings; and transplanting the tissue culture seedlings. The method provided by the invention is used for tissue culture of physochlaina and has the advantages of high reproduction speed, high inductivity and high survival rate of transplanting. The method provided by the invention is from modern development of Mongolian medicines and supplies basis and reference for the large-area cultivation, production, development and utilization of physochlaina and other generic plant resources.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture method of vesicularis. Background technique [0002] Physochlaina Physochlaina Physaloides (L.) G. Don is the dry root of the Solanaceae plant. As a Mongolian medicine "Hun-Haorisu", it was first recorded in the classic Tibetan and Mongolian medicine book "Incorrect Mongolian Medicine". Documents such as "Recognizing Medicine Baijingjian" have been recorded, which are the varieties included in the drug standards of the Ministry of Health of the People's Republic of China (Mongolian Medicine Volume) [Yunlin, Song Hongchun, Zhang Hongyu, Liu Dongming. The effects of different processing methods on the acute toxicity and the drug Effect of efficacy [J]. Chinese Journal of Ethnic Medicine. 2008.3:57~58]. There are relevant literatures on the content determination of scopolamine in vesicularis [Zhang Runxiang. Determination of the content of hyoscyamine ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王晓娟杨鼎伊风艳孙海莲赛希雅拉
Owner INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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