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Tissue culture method for physochlaina

A technology of tissue culture and vesicular grass, applied in the field of plant tissue culture, to achieve the effects of low pollution rate, strict requirements, and cost reduction

Inactive Publication Date: 2016-07-20
INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the successful tissue culture of Physiophthora vesicosa, and it is necessary to screen the appropriate culture medium of Physicum vesicularis in actual production

Method used

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  • Tissue culture method for physochlaina
  • Tissue culture method for physochlaina
  • Tissue culture method for physochlaina

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Experimental program
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Effect test

Embodiment 1

[0029] The tissue culture method of Herba vesicularis, the method comprises the following steps:

[0030] 1.1 Establishment of explants

[0031] The test material is the Physcoma vesicularis plant collected by the Grass Industry Center of Inner Mongolia Academy of Agriculture and Animal Husbandry Science in Abaga Banner, Xilin Gol League, Inner Mongolia Autonomous Region.

[0032] 1.2 Test method

[0033] 1.2.1 Sterilization of explants

[0034] Select the leaves of the current year’s healthy branches as explants, cut them into 5×5mm stem segments with a scalpel, wash the stem segments of the explants with neutral detergent for 10 minutes to wash off the surface stains, rinse them under tap water for 20 minutes, filter paper After absorbing the water, place it on a clean workbench for sterilization, and use 0.1% mercury liter for disinfection treatment. The treatment time is shown in Table 1. Rinse with sterile water for 3 to 5 times, and then inoculate in MS medium. After 2...

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Abstract

The invention discloses a tissue culture method for physochlaina. The tissue culture method comprises the following steps: sterilizing an explant; performing primary induction culture on physochlaina buds; performing subculture multiplication culture; rooting tissue culture seedlings; and transplanting the tissue culture seedlings. The method provided by the invention is used for tissue culture of physochlaina and has the advantages of high reproduction speed, high inductivity and high survival rate of transplanting. The method provided by the invention is from modern development of Mongolian medicines and supplies basis and reference for the large-area cultivation, production, development and utilization of physochlaina and other generic plant resources.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture method of Herba vesicularis. Background technique [0002] Physochlaina Physaloides (L.) G.Don is the dry root of Physochlaina Physaloides (L.) G.Don, a Mongolian medicine "Hun-Haorisu", which was first recorded in the classic Tibetan and Mongolian medicine "Wuwu Mongolian Medicine", " Recognized medicine Baijingjian" and other documents are recorded, and it is a variety recorded in the drug standard (Mongolian medicine sub-volume) of the Ministry of Health of the People's Republic of China [Yun Cailin, Song Hongchun, Zhang Hongyu, Liu Dongming. The effects of different processing methods on acute toxicity and efficacy The impact of [J]. Chinese Journal of National Medicine. 2008.3: 57 ~ 58]. There are relevant literatures on the determination of hyoscyamine content in Herba Physiosa [Zhang Runxiang. HPLC Determination of Hyoscyamine Content in Herb...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王晓娟杨鼎伊风艳孙海莲赛希雅拉
Owner INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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