Tissue culture method for physochlaina
A technology of tissue culture and vesicular grass, applied in the field of plant tissue culture, to achieve the effects of low pollution rate, strict requirements, and cost reduction
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[0029] The tissue culture method of Herba vesicularis, the method comprises the following steps:
[0030] 1.1 Establishment of explants
[0031] The test material is the Physcoma vesicularis plant collected by the Grass Industry Center of Inner Mongolia Academy of Agriculture and Animal Husbandry Science in Abaga Banner, Xilin Gol League, Inner Mongolia Autonomous Region.
[0032] 1.2 Test method
[0033] 1.2.1 Sterilization of explants
[0034] Select the leaves of the current year’s healthy branches as explants, cut them into 5×5mm stem segments with a scalpel, wash the stem segments of the explants with neutral detergent for 10 minutes to wash off the surface stains, rinse them under tap water for 20 minutes, filter paper After absorbing the water, place it on a clean workbench for sterilization, and use 0.1% mercury liter for disinfection treatment. The treatment time is shown in Table 1. Rinse with sterile water for 3 to 5 times, and then inoculate in MS medium. After 2...
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