A kind of microbial self-assembly nanomaterial and its preparation method and application

A technology of nanomaterials and microorganisms, which is applied in the field of microbial self-assembly nanomaterials and its preparation, to achieve the effects of simple operation, environmentally friendly cost, and high adaptability

Active Publication Date: 2018-09-28
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there have been no reports about the self-assembly of nano-calcium-phosphorus materials by microorganisms so far, so the self-assembly of nano-calcium-phosphorus materials through microbial technology is still a technical problem that needs to be solved in this field.

Method used

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  • A kind of microbial self-assembly nanomaterial and its preparation method and application
  • A kind of microbial self-assembly nanomaterial and its preparation method and application
  • A kind of microbial self-assembly nanomaterial and its preparation method and application

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Effect test

Embodiment 1

[0034] Example 1 Isolation and identification of Shewanella sp. CF8-6

[0035] The screening and isolation process of Shewanella sp. CF8-6 is as follows:

[0036] (1) Several bacterial strains were isolated from the South China Sea mud, inoculated part of the strains isolated from the South China Sea mud into seawater LB liquid medium, and cultivated at 25°C and 200rpm for 24 hours to obtain an activated bacterial liquid;

[0037] (2) Inoculate the activated bacterial liquid obtained in the above step (1) into simulated high-salt wastewater at a ratio of 10%, cultivate it at 25°C and 200rpm, and select the one whose removal rate is higher than 90% in 12h and 24h. Several strains were re-screened;

[0038] (3) Screen the strains obtained in step (2) again according to steps (1) and (2), increase the sampling time points, select a strain with the best phosphorus removal speed and phosphorus removal rate, and name it CF8-6.

[0039] Components of the above-mentioned seawater LB...

Embodiment 2

[0046] The preparation of embodiment 2 microbial self-assembly nanomaterials

[0047] Utilize Shewanella strain (Shewanella sp.) CF8-6 to prepare the method for nanometer material as follows:

[0048] (1) Cultivate the strain Shewanella sp.CF8-6 in seawater LB liquid medium at 25°C and 200 rpm for 24 hours to obtain an activated bacterial liquid; centrifugation parameters for the activated bacterial liquid: centrifuge at 10,000 rpm for 10 min;

[0049] (2) Centrifuge and wash the bacterial solution obtained in step (1) and put it into simulated waste water at a ratio of 10% (v / v), cultivate it for 48 hours at 25°C and 200rpm, and then centrifuge to obtain bacterial cells, nano Particles are distributed on and around the cell surface. The centrifugal speed is 4000rpm, and the time is 10 minutes;

[0050] (3) Wash the bacterium containing nanoparticles obtained in step (2) twice with deionized water at a rotation speed of 4000 rpm for 15 minutes. Then take it for transmission...

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Abstract

The invention discloses a microbial self-assembled nanometer material, its preparation method and application. Specifically, the present invention utilizes a bacterium Shewanella sp. that can efficiently remove phosphorus in high-salt wastewater under aerobic conditions, and simultaneously utilize raw materials in wastewater to synthesize calcium and phosphorus nanoparticles. CF8‑6 synthesized calcium-phosphorus nanomaterials while removing phosphorus from wastewater, especially calcium-phosphorus nanoparticles were synthesized under the condition of low phosphorus saturation. The method and the nanometer material prepared by it play an important role in the fields of removing environmental pollution, biomedicine and the like.

Description

technical field [0001] The invention belongs to the field of nanomaterial preparation, and in particular relates to a microbial self-assembled nanomaterial and its preparation method and application. Background technique [0002] Nanomaterials refer to materials in which at least one of the three-dimensional materials is in the nanoscale range or composed of them as basic units. Nanomaterials have some unique characteristics such as surface effects, small size effects, and quantum effects, so they are widely used in energy, catalysis, biosensors, biomedicine and other industries. The traditional physical and chemical synthesis methods of nanomaterials mainly include two-phase method, reverse microemulsion method, photochemical synthesis method, electrode electrolysis method, heating method, ultrasonic method, etc., but these traditional synthesis methods have various insurmountable defects. For example, the price of raw materials is high, the energy consumption is high, the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B82Y30/00C02F3/34A61K47/02A61L27/12A61L27/50C12R1/01C02F101/20
CPCA61K47/02A61L27/12A61L27/50A61L2430/02B82Y30/00C02F2101/20C12N1/20C12N1/205C12R2001/01
Inventor 周维芝王艳茹蒋丽
Owner SHANDONG UNIV
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