Method for extracting DNA (deoxyribonucleic acid) by rapidly drying plant leaves with oven

Abstract

The invention relates to a method for extracting DNA (deoxyribonucleic acid) by rapidly drying plant leaves with an oven. The method comprises the following steps: drying organs and / or tissues of a plant in vitro with microwaves (radio waves having a wavelength being 0.12m), crushing the dried organs and / or tissues of the plant, and adding a cell lysis solution into the plant powder to react for 60 to 70 minutes at 65 DEG C to obtain a DNA-containing extracting solution which is referred to as an extracting solution 1; removing impurities from the extracting solution 1 to obtain the DNA. Proved by experiments, the DNA is extracted by drying the tissues and the organs of the plant with the oven, with a short drying time of only 4 to 10 minutes; the extracted DNA can be used for SSR-PCR (Simple Sequence Repeat-Polymerase Chain Reaction), and the dried organs and / or tissues of the plant are convenient to carry, transport, post and the like, such that molecular breeding and molecular detection can be applied more widely; therefore, the method disclosed by the invention has a broad application prospect in molecular mark assisted breeding, purity detection of varieties and identification of authenticity.

Description

technical field [0001] The invention relates to a method for extracting DNA by rapidly drying plant leaves in a microwave oven in the field of biotechnology. Background technique [0002] The extraction of plant DNA is the basis for carrying out biological experiments. Generally, low-temperature preservation methods are used to preserve plant leaves to prevent DNA degradation and oxidation, such as using liquid nitrogen or ice packs, but it is inconvenient to carry. In recent years, silica gel desiccants have been used to dry plant leaves, and the dried leaves are used to extract DNA. Generally, the drying time takes 48 hours, and the desiccant needs to be replaced in the middle, which is troublesome to operate. There is an urgent need for a method that can quickly dry plant leaves to extract DNA. Contents of the invention [0003] The technical problem to be solved by the invention is how to extract plant DNA. [0004] In order to solve the problems of the technologies ...

AI Technical Summary

Problems solved by technology

[0002] The extraction of plant DNA is the basis for carrying out biological experiments. Generally, low-temperature preservation methods are used to preserve plant leaves to prevent DNA degradation and oxidation, such as using liquid nitrogen or ice packs, but it is inconvenient to carry

In recent years, silica gel desiccants have been used to dry plant leaves, and the dried leaves are used to extract DNA. Generally, the drying time takes 48 hours, and the desiccant needs to be replaced in the middle, which is troublesome to operate.

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