Preparation method of gardnerella vaginalis culture medium
The technology of Gardnerella gardnerella culture medium and vagina is applied in the field of preparation of Gardnerella vaginalis culture medium, which can solve the problems such as difficulty in obtaining, complicated preparation of double-layer culture medium, increase of pollution opportunities, etc., and achieves convenient operation and low preparation cost. , the effect of easy availability of raw materials
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[0020] Embodiments of the present invention will be further described below in conjunction with the accompanying drawings.
[0021] Please see attached figure 1 Shown, a kind of Gardnerella vaginal culture medium preparation method, this method at least comprises the following steps:
[0022] Step 1: Accurately weigh 4.1g of Columbia blood agar base, add 95ml of distilled water and heat to dissolve.
[0023] Step 2: Autoclave the lysate from Step 1.
[0024] Step 3: Cool the solution to about 50°C and add 5ml of defibrinated rabbit blood and combined bacteriostatic agent in aseptic operation.
[0025] Step 4: Aseptically pour it into a sterilized glass plate to prepare a rabbit blood Gobbit agar monolayer medium, and keep it cold for later use.
[0026] Step 5: Inoculate the Gardnerella vaginalis on the prepared rabbit blood Gobbit agar monolayer medium, and inoculate in divisions and lines.
[0027] Step 6: Incubate at 35°C, 5% CO2 for 48 hours.
[0028] In the step 1, t...
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