Preparation method of gardnerella vaginalis culture medium

Abstract

The invention discloses a preparation method of a gardnerella vaginalis culture medium, comprising the steps of: step 1, accurately weighing 4.1g of Columbia blood agar base, and adding 95ml of distilled water for dissolving with heating; step 2: autoclaving the dissolved solution of the step 1; step 3: cooling the dissolved solution to about 50 DEG C, adding 5ml of defibered rabbit blood and combined bacteriostatic agent under the condition of sterile operation; step 4, pouring the solution into a sterilized glass plate, regarding the solution as a rabbit blood Columbia agar monolayer culture medium, cooling the solution for later use; step 5: inoculating the prepared rabbit blood Columbia agar monolayer culture medium with gardnerella vaginalis, carrying out partitioned streak inoculation; and step 6: carrying out incubation at 35 DEG C with 5% CO2 for 48 hours. The preparation method only uses two components namely the rabbit blood and Colombia blood agar as nutrient substances to prepare the monolayer culture medium, can achieve the effect of separating the gardnerella vaginalis, realizes the characteristics of low cost, convenient operation, easy availability of raw materials and the like in preparation of culture media, and is favorable for wide development in different levels of hospitals.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a preparation method of a culture medium of Gardnerella vaginalis. Background technique [0002] Gardnerella vaginalis (gardnerella vaginalis GV) content in bacterial vaginosis microbial flora is significantly higher than other normal flora, has always been considered by domestic and foreign scholars as one of the important pathogenic bacteria of bacterial vaginosis. GV infection can also cause adverse pregnancy outcomes such as tubal ectopic pregnancy, premature rupture of membranes, chorioamnionitis, puerperal infection, endometritis, and neonatal premature delivery. [0003] The isolation and culture method of GV is still the internationally recognized gold standard for GV detection. The growth conditions of GV are relatively harsh, and a variety of nutrients are required to participate in the growth process. Ordinary sheep blood plates, after 35~37℃, 5% CO2, 48~72h culture, wil...

AI Technical Summary

Problems solved by technology

[0004] At present, most literatures at home and abroad use double-layer medium containing human blood plus various nutrients to isolate Gardnerella vaginalis. In the case of serious shortage of blood supply in central blood stations across the country, human blood is expensive and difficult to obtain.

The preparation of double-layer medium is complex and increases the chance of contamination

The addition of nutritional ingredients such as heart and brain infusion and yeast extract lead to an increase in cost

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