Manufacturing method of epidermis for Tillandsia epidermis structure observation

A technology for aerial Tillandsia and plant epidermis, which is applied in the field of epidermis production for the observation of aerial Tillandsia plant epidermis structure, and can solve the problems of inability to obtain ideal observation materials for aerial Tillandsia plant leaf epidermis, low scale removal, and epidermal cell clarity To improve the definition of epidermal cells and pores, the effect is outstanding, and the method is simple

Inactive Publication Date: 2016-09-07
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the epidermis production methods in the prior art include direct peeling method, boiling peeling method, isolation method, nail polish imprinting method, scotch tape sticking method, and scraping method. Although most of the methods can obtain a complete epidermis, there are certain defects. Defects, such as low scale removal, poor definition of epidermal cells and stomata, etc., cannot obtain ideal materials for observation of aerial Tillandsia plant leaf epidermis

Method used

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  • Manufacturing method of epidermis for Tillandsia epidermis structure observation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Take fresh mature leaves of "Velutina" and soak them in 3% hydrogen peroxide solution for 2.0 hours at room temperature.

[0026] 2. Take out the leaves after soaking, and select an area of ​​2cm x 2cm on the upper surface and the middle of the lower surface, respectively, and use a single-sided blade to scrape off the surface scales. At this time, the scales are easy to scrape off and not easy to scratch the epidermis.

[0027] 3. Cut off the part of the blade where the scales have been scraped off.

[0028] 4. Use tweezers to tear off the epidermis on the upper or lower surface of the cut blade, and at this time, the complete epidermis without damage can be torn off.

[0029] 5. Wash the peeled off skin layer with clean water.

[0030] 6. Immerse the torn epidermis in a drop of water on a glass slide, flatten, cover slip and carry out routine staining.

[0031] Through the above steps, the clear epidermal cell structure of the leaves of "Velutina" can be observed...

Embodiment 2

[0033] All the methods are the same as in Example 1, except that the species of the aerial Tillandsia plant is "Bawang", and step 1 is soaked with 1% hydrogen peroxide solution for 2.0 hours.

Embodiment 3

[0035] All the methods are the same as in Example 1, except that the species of the aerial Tillandsia genus is "Bronze Air Flower", and in step 1, soak with 5% hydrogen peroxide solution for 0.5 hour.

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Abstract

The invention discloses a manufacturing method of epidermis for Tillandsia epidermis structure observation. The manufacturing method comprises 1, taking fresh mature leaves and immersing the leaves in a hydrogen peroxide solution at the room temperature, 2, taking out the leaves and scraping surface exfoliate in the middles of the upper and lower surfaces, 3, cutting the leaf parts without exfoliate and tearing down the epidermis of the upper or lower surface, and 4, cleaning the epidermis by clear water, dipping the epidermis into water drops on a glass slide, unfolding the epidermis, covering the epidermis with cover glass and carrying out conventional dyeing. Compared with the prior art, the manufacturing method can take the whole epidermis, substantially improve an exfoliate removal rate and improve epidermis cell clarity and pore clarity, has simple processes and outstanding effects and is especially suitable for Tillandsia.

Description

technical field [0001] The invention relates to a method for making a skin, in particular to a method for making a skin for observing the structure of the skin of plants of the genus Tillandsia. Background technique [0002] Tillandsia is a perennial evergreen herb belonging to Bromeliaceae Crassulaceae Acid (CAM) and belongs to rootless plants. This genus plays an important role in stress resistance and gas pollutant adsorption. my country has only begun to introduce and cultivate them in recent years. Due to the strong adsorption capacity of such plants to formaldehyde and other gas pollutants, they have broad application prospects in the field of indoor greening. Because it grows in the air and has nothing to do with the soil, unlike other plants, the leaf is the only organ for absorbing water and nutrients, and the leaf structure is special. In order to facilitate the more scientific application of this type of plant, the study of the leaf epidermis structure is urgently...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/2813G01N1/30
Inventor 管斌沈正宏鲍荣静周道宏汤荣弟李冰
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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