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Clyz and hltf double antibacterial gene recombinant adenovirus construction method and recombinant adenovirus and application

A technology of recombinant adenovirus and construction method, which is applied in the field of constructing recombinant adenovirus, can solve the problems such as the difficulty in the treatment of bacterial drug-resistant endemic cow mastitis

Active Publication Date: 2020-03-27
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the prevention and treatment of the disease is mainly through standardized breeding management and the use of antibiotics and chemotherapy drugs. Difficulties in the treatment of endemic dairy cow mastitis

Method used

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  • Clyz and hltf double antibacterial gene recombinant adenovirus construction method and recombinant adenovirus and application
  • Clyz and hltf double antibacterial gene recombinant adenovirus construction method and recombinant adenovirus and application
  • Clyz and hltf double antibacterial gene recombinant adenovirus construction method and recombinant adenovirus and application

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Embodiment Construction

[0021] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0022] With the development of molecular biology, immunology, pathology and related disciplines, gene therapy has become a hot spot in the research of disease prevention and treatment. At present, adenovirus (adenovirus, Ad) vector therapy is one of the most promising gene transfer methods in gene therapy. It can effectively deliver foreign genes to various target cells or tissues. The construction and operation of the vector are simple, It has a wide host range, strong infectivity, does not integrate into the host chromosome, and can infect and express a variety of target genes in both proliferating and non-proliferating cells.

[0023] Fish is the most primitive and lowest group in the vertebrate subphylum. Although it has specific and non-specific immune systems, compared with mammals, its specific immune system is underdeveloped. Therefore,...

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Abstract

The invention discloses an establishment method of a cLYZ / hTLF double-antibacterial gene recombinant adenovirus, and a recombinant adenovirus and application thereof. The method comprises the following steps: respectively amplifying cLYZ gene and hTLF gene from grouper head-kidney tissues and human milk somatic cell total RNAs, cloning the obtained goal genes into a pDC315 shuttle vector, co-transfecting HEK293 cells through liposome by using the target-gene-containing shuttle plasmid and adenovirus framework plasmid, and packaging to obtain the high-titer recombinant adenovirus; and evaluating the combined inhibiting actions of the cLYZ gene and hTLF gene on the milk cow mastitis primary pathogens under the adenovirus mediated conditions.

Description

technical field [0001] The invention relates to a method for constructing a recombinant adenovirus, specifically, a method for constructing a recombinant adenovirus with cLYZ and hLTF dual antibacterial genes, the recombinant adenovirus and its application. Background technique [0002] Cow mastitis, also known as cow mastitis, is an inflammatory reaction that occurs when the cow's mammary gland is stimulated by the outside world or infected by pathogenic bacteria. It will not only cause a decline in milk production of dairy cows, shorten the replacement cycle of dairy cows, and even cause the complete loss of lactation ability of sick cows in severe cases. Therefore, the clinical prevention and treatment of mastitis in dairy cows has been a major problem that has plagued the dairy farming industry. At present, the prevention and treatment of the disease is mainly through standardized breeding management and the use of antibiotics and chemotherapy drugs. It brings difficul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/01C12N15/861A61K38/47A61K38/40A61P31/04C12R1/93
CPCC07K14/79C12N7/00C12N9/2462C12N15/86C12N2710/10021C12N2710/10043C12N2800/107C12Y302/01017
Inventor 金天明弓建芳张东超李小慧高珂珂林静孟佳丽尚翠玲李昕
Owner TIANJIN AGRICULTURE COLLEGE