A kind of sample preparation method of scanning electron microscope

Abstract

The invention discloses a preparation method of a scanning electron microscope sample. The preparation method comprises the following steps: treating a feces sample, wrapping and washing the feces sample in a nylon bag, standing and washing the feces sample in a culture dish, flushing by using a high-speed water flow, changing water, drying by absorbing water, preparing a sample platform, and coating the sample with a film by gold sputtering. The scanning electron microscope sample prepared by drying, crushing, soaking, wrapping and washing in the nylon bag, flushing by using the high-speed water flow, absorbing water, drying, plating gold and other processes is clean, free of impurities and gravel deposits, small in residue loss and uniform in distribution; during macroscopic observation, the residue sample is clear, and during observation under a scanning electron microscope, a residue structure is clear and complete, the view is clean and the third dimension of an image is strong; by the preparation method, observation and research on ultrastructure of an undigested forage grass residue in digested feces of a ruminant animal can be facilitated; the preparation method has an important significance in vividly understanding and evaluating degradation of microbes in the digestive tract of the ruminant animal to a roughage fiber structure and raising the utilization rate of straw feed.

Description

technical field [0001] The invention belongs to the technical field of microscopic observation samples, and relates to a scanning electron microscope (SEM) sample preparation method, in particular to a scanning electron microscope (SEM) observation sample preparation method for forage residues in feces after digestion by ruminants. Background technique [0002] In recent years, with the rapid economic and social development and the continuous improvement of people's living standards, my country's animal husbandry has developed rapidly. Therefore, the requirements for the quantity and quality of forage resources are constantly improving, and relevant research is being carried out vigorously. Under this major development trend, the improvement of forage utilization rate is becoming a research hotspot, and various technologies to promote forage digestibility are emerging one after another. At present, most of the research results of forage digestibility are only reflected and ...

AI Technical Summary

Problems solved by technology

At present, most of the research results on the degree of forage digestion are only reflected and compared with quantitative indicators, such as digestibility, rumen degradation rate, rumen disappearance rate, etc. There is generally a lack of fiber structure and cell wall changes that can directly reflect the forage passing through the digestive tract of ruminants Visual image data of