Novel TRAIL fusion protein

A fusion protein and protein technology, which can be used in peptide/protein components, animal/human proteins, targeting specific cell fusion, etc., and can solve the problem of not having cell division and proliferation activity.

Active Publication Date: 2016-10-26
INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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  • Abstract
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Problems solved by technology

The third loop region of TGF-α (TGF3L) can bind to EGFR, but it does

Method used

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  • Novel TRAIL fusion protein

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Embodiment Construction

[0052] The purpose of the present invention is to provide a new type of TRAIL fusion protein, a method for preparing a new type of TRAIL fusion protein, its coding gene, and expression vector, the steps of expressing the fusion protein coding gene and expression vector, and a new type How to use TRAIL fusion protein.

[0053] The present invention found that TRAIL protein is connected with the third loop region of TGF-α (TGF3L) to form a new fusion protein, which has a stronger tumor cell killing effect than TRAIL protein; this enhancement effect is mainly reflected in tumor cells sensitive to TRAIL , And normal cells that are not sensitive to TRAIL toxicity are still inactive. For many TRAIL-sensitive human tumor cells, the specific tumor-killing effect of the fusion protein can be increased by a hundred-fold or even a thousand-fold; for mouse tumor L929 cells, the tumor-killing effect of the fusion protein can still be increased by more than one hundred-fold. The extremely hig...

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Abstract

The invention provides a novel TRAIL fusion protein, which belongs to the field of biotechnology. The novel TRAIL fusion protein contains a third loop sequence of TGF-alpha and has strong specific tumor killing effect. The invention also discloses a coding gene of the TRAIL fusion protein and an expression pathway thereof, a production method for the fusion protein and application of the fusion protein in treatment of diseases like tumors.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and relates to a novel TRAIL fusion protein, a method for producing the fusion protein using genetic engineering technology, and its application as a medicine to treat tumors and other related diseases. Background technique [0002] TRAIL (TNF-related apoptosis-inducing ligand), also known as Apo2L (Apo-2ligand, apoptin-2 ligand), is a member of the tumor necrosis factor superfamily [1] , It was officially named Tumor necrosis factor ligand superfamily member 10 (Tumor necrosis factor ligand superfamily member 10). TRAIL molecule is composed of 281 amino acid residues, is a typical type II transmembrane protein, 18-38 amino acid residues form a single transmembrane region with a helical structure, the amino terminal is located in the cytoplasm, and the carboxyl segment is 39-281 Amino acid residues are located outside the cell. The extracellular part of TRAIL can be cleaved by proteases and released int...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62A61K38/17A61K47/48A61P35/00
CPCA61K38/00C07K14/495C07K14/70575C07K2319/33
Inventor 王楠闫征王燕
Owner INST OF MATERIA MEDICA AN INST OF THE CHINESE ACAD OF MEDICAL SCI
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