Oocyte aggregation device

Abstract

The invention discloses an oocyte aggregation device. The oocyte aggregation device comprises an aggregation ring (1), an aggregation ring handle (2), a lock spring clamp (3), a plastic clamp ring (4) and an oocyte pushing needle (5); the aggregation ring (1) and the aggregation ring handle (2) are of an integral structure, the aggregation ring (1) is fixed into a ring shape through the lock spring clamp (3), the size of the aggregation ring (1) is adjustable, and the oocyte pushing needle (5) is connected with the aggregation ring handle (2) through the plastic clamp ring (4). By adopting the oocyte aggregation device, an appropriate quantity of free oocytes which are uniformly distributed can be quickly and conveniently aggregated on the condition that the shape structure of the oocytes is not influenced when the oocytes are shot by an anatomical lens or a microscope, therefore, images of the appropriate quantity of free oocytes which are uniformly distributed can be shot in a single image, oocyte image collection is quickly completed, and experimental cost and capital cost are reduced.

Description

technical field [0001] The invention relates to an experimental equipment, specifically designs an oocyte accumulator, in particular to an oocyte accumulator for image acquisition of oocytes such as cephalopods and fishes. Background technique [0002] Research on reproductive biology of cephalopods and fish often involves their fecundity, gametogenesis, gamete size and structure, etc., and provides a basis for research on reproductive strategies. With the development and application of image acquisition and analysis software in the current biotechnology field (such as Image-Pro Plus series of professional image analysis software), it provides a lot for the calculation of the fecundity of cephalopods, fish and other species, and the measurement of oocyte size. Great convenience. [0003] However, when using such image analysis software for fecundity calculation, oocyte size measurement, etc., it is necessary to collect oocyte images under a dissecting mirror or microscope. ...

AI Technical Summary

Problems solved by technology

Due to the limited number of oocytes that can be collected in a single image by a dissecting mirror or microscope, if only 1-2 oocytes are captured in a single image, it will bring a lot of time cost to the experiment, and it will also make the experiment difficult to upgrade. Capital cost of computer hard drive space to store all images acquired

At the same time, if too many densely distributed oocytes are ingested in a single image, it will bring inconvenience or data deviation for the subsequent application of professional image analysis software for automatic calculation of the number and automatic measurement of oocyte size. The original structure and shape of oocytes are definitely destroyed due to extrusion

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