Small microbial ecosystem method for preparing multifunctional compound microorganism bacterium agent and application
A composite microbial agent and ecosystem technology, which is applied in the fields of biomanufacturing, agriculture and the environment, can solve the problems of weak synergistic effect, single function of microbial agent, and inability to produce
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Embodiment 1
[0067] Embodiment 1 specifically prepares the composite microbial bacterial agent product: the strains used in this embodiment come from the strain preservation center of the Institute of Microbiology, Chinese Academy of Sciences, and the strains and numbers are: Bacillus subtilis CGMCC1.1086, Bacillus polymyxa CGMCC1.0548, Bacillus megaterium CGMCC1.0217, Bacillus stearothermophilus CGMCC1.3380, Aspergillus niger CGMCC3.0316, Cystis coronis CGMCC3.0448.
[0068] Use the following steps to achieve:
[0069] Step 1: Establish a small microbial ecosystem, including natural culture preparation, initial culture solution preparation, initial culture solution sterilization, sterility test, small micro-ecosystem formation and mixed-bacteria seed production;
[0070] (1) Natural culture preparation:
[0071] ①According to the weight percentage, the substances and proportions contained in 1000g natural culture are: 40% of fresh animal feces, 10% of activated sludge, 10% of natural soi...
Embodiment 2
[0092] Example 2 Application of multifunctional composite microbial bacterial agent in the field of agriculture and environment: The multifunctional composite microbial bacterial agent prepared by the present invention has good biological enzyme activity. Enzyme, urease, protease, pectinase, xylanase, superoxide dismutase (SOD), lipase, phytase and other enzyme activities are very high, and the activity of various enzymes is between 280-350U / g ; Biological toxins such as aflatoxins (B1, B2, G1, G2), ochratoxins, mycotoxins, etc. were not detected; indicators related to biological fertilizers and biological fermentation agents stipulated by other countries are all qualified. The main application mode of the multifunctional composite microbial bacterial agent prepared by the present invention can be: (1) as an organic material decomposing agent to treat solid organic waste, and realize the resource utilization of solid organic waste; (2) as an ecological feed additive ; (3) Used...
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