Small microbial ecosystem method for preparing multifunctional compound microorganism bacterium agent and application
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A composite microbial agent and ecosystem technology, which is applied in the fields of biomanufacturing, agriculture and the environment, can solve the problems of weak synergistic effect, single function of microbial agent, and inability to produce
Inactive Publication Date: 2016-11-09
中山市润泽生物科技有限公司
View PDF3 Cites 2 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0006] In order to overcome the defects of the prior art, the present invention provides a method for preparing a multifunctional composite microbial inoculum using a small-scale microbial ecosystem method, and simultaneously provides a multifunctional composite microbial inoculum prepared by the above-mentioned method and its application; it can solve the exi
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0067] Embodiment 1 specifically prepares the composite microbial bacterial agent product: the strains used in this embodiment come from the strain preservation center of the Institute of Microbiology, Chinese Academy of Sciences, and the strains and numbers are: Bacillus subtilis CGMCC1.1086, Bacillus polymyxa CGMCC1.0548, Bacillus megaterium CGMCC1.0217, Bacillus stearothermophilus CGMCC1.3380, Aspergillus niger CGMCC3.0316, Cystis coronis CGMCC3.0448.
[0068] Use the following steps to achieve:
[0069] Step 1: Establish a small microbial ecosystem, including natural culture preparation, initial culture solution preparation, initial culture solution sterilization, sterility test, small micro-ecosystem formation and mixed-bacteria seed production;
[0070] (1) Natural culture preparation:
[0071] ①According to the weight percentage, the substances and proportions contained in 1000g natural culture are: 40% of fresh animal feces, 10% of activated sludge, 10% of natural soi...
Embodiment 2
[0092] Example 2 Application of multifunctional composite microbial bacterial agent in the field of agriculture and environment: The multifunctional composite microbial bacterial agent prepared by the present invention has good biological enzyme activity. Enzyme, urease, protease, pectinase, xylanase, superoxide dismutase (SOD), lipase, phytase and other enzyme activities are very high, and the activity of various enzymes is between 280-350U / g ; Biological toxins such as aflatoxins (B1, B2, G1, G2), ochratoxins, mycotoxins, etc. were not detected; indicators related to biological fertilizers and biological fermentation agents stipulated by other countries are all qualified. The main application mode of the multifunctional composite microbial bacterial agent prepared by the present invention can be: (1) as an organic material decomposing agent to treat solid organic waste, and realize the resource utilization of solid organic waste; (2) as an ecological feed additive ; (3) Used...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a small microbial ecosystem method for preparing a multifunctional compound microorganism bacterium agent and application. The method comprises the following steps that firstly, a small microorganism ecosystem is built, three or more mutually non-antergic microorganism seeds are inoculated to the small microorganism ecosystem for mixed culture, various multiple microorganism seeds with microorganism complex enzymes are obtained, then, fungi for decomposing different plant material enzymes are cultured on the basis of a conventional method, fungus shake flask seeds are obtained, then, various multiple microorganism seeds and the fungus shake flask seeds are inoculated to a solid nutrient medium for solid state fermentation, and after fermentation is finished, the multifunctional compound microorganism bacterium agent is prepared. The method can solve the problems that an existing microorganism bacterium agent applied to agriculture and environment is single in function and poor in adaptability, the bacterium synergistic effect capacity is poor, no novel or multifunctional complex enzymes are generated, and the production cost is high, and low-cost and efficient cleaning processing and resource utilization of agricultural and environmental pollution waste can be achieved.
Description
technical field [0001] The invention belongs to the technical fields of biomanufacturing, agriculture and environment, and relates to a composite microbial bacterial agent and its application, in particular to the preparation of a multifunctional composite microbial bacterial agent and its application by a small microbial ecosystem method. Background technique [0002] At present, animal excrement and solid waste generated by human production activities are increasingly polluting the environment, and the harmless treatment and resource utilization of these pollutants are facing great challenges. [0003] In the field of agriculture and the environment, various microbial products and biological agents for the harmless treatment and resource utilization of pollutants are constantly emerging, with the following characteristics: (1) Most microbial products have low technical content, similar products, and simple preparation Extensive; (2) There are relatively few excellent new s...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more