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Cell culture dish for radioactive biological experiment and experimental device containing cell culture dish

A technology of cell culture and radiobiology, applied in biochemical cleaning devices, enzymology/microbiology devices, stress-stimulated microbial growth methods, etc., can solve the interference of cell survival curve calculation, difference in survival rate of control objects, experimental errors, etc. problems, to achieve a high degree of uniformity in irradiation dose, to achieve uniformity in irradiation dose, and to avoid experimental errors

Inactive Publication Date: 2016-11-16
李夏东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] (1) In the existing cell culture dish, because the aperture of the culture chamber is very small, under the action of water surface tension, the water level at the wall of the culture chamber will be higher than that in the middle of the culture chamber, which results in the use of existing irradiation methods for irradiation Even if the control object (adherent cells) in the same culture chamber has a millimeter-level difference in the actual irradiation depth between the center and the edge, because the cell thickness is in the micron level, the millimeter-level difference will cause a difference in the same culture chamber. The control object (adherent cells) has a great deviation in the irradiated dose;
[0009] (2) Cavity structures with different geometric shapes exist between different culture chambers, resulting in large differences in the degree of charged particle balance in different culture chambers, resulting in significant differences in the survival rate of control objects in different culture chambers, As a result, it greatly interferes with the calculation of cell survival curves in radiobiological experiments;
[0010] (3) It is impossible to make the amount of culture solution in each culture chamber consistent when artificially adding culture solution, and even there is a large difference, thus resulting in different depths of rays from the culture solution to the adherent cells at the bottom of different culture chambers. As a result, the absorbed doses of the control objects (adherent cells) in different culture chambers are different, resulting in obvious experimental errors

Method used

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  • Cell culture dish for radioactive biological experiment and experimental device containing cell culture dish
  • Cell culture dish for radioactive biological experiment and experimental device containing cell culture dish
  • Cell culture dish for radioactive biological experiment and experimental device containing cell culture dish

Examples

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Embodiment 1

[0057] This embodiment provides a cell culture dish for radiation biology experiments, which includes a culture dish body 100, and the material of the culture dish body 100 is solid water.

[0058] Such as image 3 As shown, the culture dish body 100 includes a fixing plate 110 , and a plurality of culture cavities 120 are arranged in the middle of the fixing plate 110 , and connection edges 130 are formed between the surroundings of the multiple culture cavities 120 and the corresponding edges of the fixing plate 110 .

[0059] Such as Figure 4 shown. All the connecting edges 130 are bent toward the same side to form an integrated reinforcing ring 210 , and at least one reinforcing plate is provided between any connecting edge 130 and the reinforcing ring 210 .

[0060] In this embodiment, the at least one reinforcing plate includes a first reinforcing plate 141 arranged at the joint of any adjacent connecting edge 130 and two second reinforcing plates 142 symmetrically ar...

Embodiment 2

[0063] This embodiment provides an experimental device for radiation biology experiments, which includes the petri dish body 100 in embodiment 1, and also includes a petri dish seat body 300 .

[0064] Such as Figure 5 , 6 As shown, the petri dish holder body 300 has an experimental chamber 310 for placing the petri dish body 100 and a water storage chamber 320 for holding clear water, and a first water pump 331 and a first water pump 331 and a The second water pump 332 forms a circulating water channel.

[0065] In this embodiment, the experimental cavity 310 is provided with a support column 311 at any corner corresponding to the outer edge of the culture dish body 100, and at least one positioning block 312 is provided on the inner wall of the experimental cavity 310, and the at least one positioning block 312 is used for cultivating The dish body 100 is pressed against the support column 311 , and the positioning block 312 includes a fixing seat 312 a and an elastic pre...

Embodiment 3

[0068] This embodiment provides a kind of irradiation method used in radiobiological experiments, which comprises the following steps:

[0069] Step 1, multiple groups of adherent cells to be irradiated are respectively placed inside the bottom walls of different culture chambers 120 of the culture dish body 100 having multiple culture chambers 120, the bottom walls of the multiple culture chambers 120 have the same thickness and On the same horizontal plane, the culture dish body 100 is made of solid water material;

[0070] Step 2, place the petri dish body 100 processed in step 1 in a petri dish seat body 300, the petri dish seat body 300 has at least one experimental cavity 310 for placing the petri dish body 100, the bottom of the petri dish seat body 300 The wall is at least parallel to the bottom wall of the plurality of culture chambers 120 and transparent, and then put clear water in the experiment chamber 310, and ensure that the water level in the experiment chamber...

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Abstract

The invention relates to the technical field of radioactive biological experiment measures, in particular to a cell culture dish for a radioactive biological experiment and an experimental device containing the cell culture dish, and provides the cell culture dish and the experimental device containing the cell culture dish. The cell culture dish is made of a solid water material, the experimental device further comprises a culture dish seat body, an experiment cavity for containing the culture dish body and a water storage cavity for containing clear water are formed in the culture dish seat body, and a circulating water path is formed between the experiment cavity and the water storage cavity through a first water pump and a second water pump. According to the cell culture dish and the experimental device containing the cell culture dish, electronic balance of irradiation light at a controlled object (anchorage-dependent cells) can be achieved well.

Description

technical field [0001] The invention relates to the technical field of radiation biology experiment equipment, in particular to a cell culture dish for radiation biology experiment and an experiment device comprising the culture dish. Background technique [0002] Radiation therapy has become one of the important means of tumor treatment for nearly a hundred years. According to the data of the National Cancer Center of the United States, 80% of patients need radiation therapy at various stages of tumor treatment, and radiobiological experiments are the real use of radiation in clinical practice. A necessary course before radiotherapy for tumors. With the research and development of chemotherapeutic drugs, molecular targeted drugs, other radioprotectants and radiosensitizers, every cancer treatment center, cancer research institute, and tumor radiotherapy center at home and abroad needs to conduct radiobiological experiments to test different drugs and radiosensitizers. Scie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/42C12M1/22C12M1/00
CPCC12M23/10C12M23/22C12M23/34C12M35/02C12M41/00
Inventor 李夏东毛立俊张仕蓉
Owner 李夏东