Irradiation method used in radiobiological experiment

Abstract

The invention relates to the technical field of radiobiological experiment methods and relates to an irradiation method used in radiobiological experiment. The irradiation method comprises the following steps: 1) placing a plurality sets of to-be-irradiated adherent cells respectively onto the inner sides of bottom walls of different culturing chambers in a culture dish body having a plurality of the culturing chambers, wherein the inner sides of the bottom walls of the culturing chambers are in the same horizontal plane, and the culture dish body is made of a solid water material; 2) placing the culture dish body into a culture dish base body which is provided with at least one experimental chambers for arranging the culture dish body, wherein the bottom wall of the culture dish base body is at least in parallel with the inner sides of the bottom walls of the culturing chambers and is transparent, and adding clear water into the experimental chamber; and 3) placing the culture dish base body into an irradiation device, controlling the irradiation ray to be vertical to the bottom wall of the culture dish base body so as to irradiate the bottom walls of the culturing chambers from bottom to top. The method ensures the equal irradiation depth to the adherent cells.

Description

technical field [0001] The invention relates to the technical field of radiation biological experiment methods, in particular to an irradiation method used in radiation biological experiments. Background technique [0002] Radiation therapy has become one of the important means of tumor treatment for nearly a hundred years. According to the data of the National Cancer Center of the United States, 80% of patients need radiation therapy at various stages of tumor treatment, and radiobiological experiments are the real use of radiation in clinical practice. A necessary course before radiotherapy for tumors. With the research and development of chemotherapeutic drugs, molecular targeted drugs, other radioprotectants and radiosensitizers, every cancer treatment center, cancer research institute, and tumor radiotherapy center at home and abroad needs to conduct radiobiological experiments to test different drugs and radiosensitizers. Scientific and rigorous experimental research ...

AI Technical Summary

Problems solved by technology

[0008] (1) In the existing cell culture dish, because the aperture of the culture chamber is very small, under the action of water surface tension, the water level at the wall of the culture chamber will be higher than that in the middle of the culture chamber, which results in the use of existing irradiation methods for irradiation Even if the control object (adherent cells) in the same culture chamber has a millimeter-level difference in the actual irradiation depth between the center and the edge, because the cell thickness is in the micron level, the millimeter-level difference will cause a difference in the same culture chamber. The control object (adherent cells) has a great deviation in the irradiated dose;

[0009] (2) Cavity structures with different geometric shapes exist between different culture chambers, resulting in large differences in the degree of charged particle balance in different culture chambers, resulting in significant differences in the survival rate of control objects in different culture chambers, As a result, it greatly interferes with the calculation of cell survival curves in radiobiological experiments;

[0010] (3) It is impossible to make the amount of culture solution in each culture chamber consistent when artificially adding culture solution, and even there is a large difference, thus resulting in different depths of rays from the culture solution to the adherent cells at the bottom of different culture chambers. As a result, the absorbed doses of the control objects (adherent cells) in different culture chambers are different, resulting in obvious experimental errors

Images