Preparation method and application of ginseng stem and leaf total saponin contrast extractive

A technology of ginseng stems and leaves and ginsenosides, which is applied in the field of preparation of reference extracts of total saponins from ginseng stems and leaves, can solve the problems of high price and shortage of component chemical reference substances, reduce pesticide residues, promote the intensification of the traditional Chinese medicine industry, and improve the content high effect

Inactive Publication Date: 2016-11-23
CHANGCHUN UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method and application of a reference extract of total saponins from ginseng stems and leaves, which is used to identify various saponin components, so as to overcome the shortcomings and deficiencies of the shortage and high price of chemical reference substances for each component

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  • Preparation method and application of ginseng stem and leaf total saponin contrast extractive
  • Preparation method and application of ginseng stem and leaf total saponin contrast extractive
  • Preparation method and application of ginseng stem and leaf total saponin contrast extractive

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Embodiment 1

[0027] A method for preparing a control extract of total saponins from ginseng stems and leaves, comprising the following steps:

[0028] 1) Extraction: Cut the ginseng stems and leaves into small pieces of about 2cm, soak in water for 0.5h, add water to decoct 3 times, add 16 times the amount of water each time, decoct for 1 hour each time, combine three decoction liquids, and let stand. Filter to obtain filtrate I;

[0029] 2) Refining and purification: Pass the filtrate through a styrene skeleton D101 macroporous adsorption resin column, wash with water until it is colorless, and then elute with 60% ethanol. The ethanol elution flow rate is 1.5-2.0 column volume multiples / h. Start to collect when the color appears or when the ethanol flows out. Add 20% magnesium oxide by weight of the raw material of ginseng stems and leaves, stir, heat to boiling, cool, let stand for 12-18h, centrifuge and filter, and then filter through 0.2μm membrane frame. Filtrate II;

[0030] 4) Concentrat...

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Abstract

The invention provides a preparation method of ginseng stem and leaf total saponin contrast extractive. The preparation method comprises the following steps of extracting, refining and purifying, concentrating and drying and content measuring. The invention further discloses application of the ginseng stem and leaf total saponin contrast extractive to identification and content measurement of total saponins in ginseng stem and leaf preparations. The ginseng stem and leaf total saponins contrast extractive is sufficient in extraction, high in active ingredient content and low in content of pesticide residues and other harmful matter, the clinical effect of a ginseng preparation with the extractive as a raw material is better, the extractive can be used for identification and content measurement of the total saponins in the ginseng stem and leaf preparations, and industrialization of the extractive has important significance for reasonably utilizing traditional Chinese medicine resources, improving the overall technology of the traditional Chinese medicine industry and promoting intensification of the traditional Chinese medicine industry.

Description

Technical field [0001] The invention relates to the field of traditional Chinese medicine preparations, in particular to a preparation method and application of a control extract of total saponins of ginseng stems and leaves. Background technique [0002] The stems and leaves of ginseng are the dry leaves of Panax ginseng C.A.Mey. Return to the spleen, lung, heart and kidney channels. To invigorate vitality, rejuvenate the pulse, nourish the spleen and lungs, nourish the body fluid and nourish blood, soothe the nerves and improve the mind. It is used for physical weakness and desire to get off, cold limbs and weak pulse, spleen deficiency and lack of food, lung deficiency and cough, body and wound thirst, internal heat to reduce thirst, deficiency of qi and blood, chronic illness and deficiency, palpitations, insomnia, impotence and cold palace. As early as 1854, some people began to study the chemical composition of ginseng. During this century, scholars from Asia including Ch...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/02A61K36/258
CPCG01N30/90A61K36/258A61K2236/331A61K2236/39A61K2236/51A61K2236/55G01N30/02G01N2030/027
Inventor 贡济宇蔡广知沈晓君王瑶郭云龙张美玲王玥玥
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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