Perfluorocarbon emulsion and preparing method and application thereof

A perfluorocarbon and emulsion technology, applied in the field of biomedicine, can solve the problems of limited therapeutic efficacy, hypoxia of tumor tissue, necrosis of hypoxic area, etc., and achieve the effects of high oxygen carrying capacity, improvement of hypoxia, and improvement of therapeutic effect.

Inactive Publication Date: 2016-12-07
JE & NA BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Moreover, with the rapid expansion of tumor volume, a part of the tumor tissue in the tumor is getting farther and farther away from the nearest blood vessel, which leads to insufficient tumor blood supply, which eventually leads to hypoxia in this part of the tumor tissue.
Therefore, clinically most malignant

Method used

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  • Perfluorocarbon emulsion and preparing method and application thereof
  • Perfluorocarbon emulsion and preparing method and application thereof
  • Perfluorocarbon emulsion and preparing method and application thereof

Examples

Experimental program
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Example Embodiment

[0030] Example 1: Preparation of perfluorocarbon emulsion

[0031] Weigh 40 mg of protein and dissolve it in 4 mL of phosphate buffer. After adding 200 μL of perfluorocarbon liquid, sonicate it with a cell disruptor for 5 minutes. After centrifugation, remove the lower layer and dissolve it in phosphate buffer for use. The particle size of perfluorocarbon particles was measured with a dynamic light scattering instrument, and the results are shown in figure 1 .

[0032] figure 1 The results show that the size of the synthesized perfluorocarbon particles is about 200nm.

Example Embodiment

[0033] Example 2: Determination of oxygen content change

[0034] After the perfluorocarbon emulsion in Example 1 was filled with oxygen, it was added to deionized water, and its oxygen content was measured by an oxygen electrode. After the oxygen content is stable, the coupling agent is applied to the bottom of the round-bottomed flask and then an ultrasonic probe is added to detect the change value of the oxygen content under the action of ultrasound. figure 2 .

[0035] figure 2 The results show that the oxygen-carrying capacity of perfluorocarbon emulsion is significantly higher than that of aqueous solution after filling with oxygen. Under the action of ultrasound, oxygen will have a rapid release process.

Example Embodiment

[0036] Example 3: Using immunofluorescence probes to detect changes in oxygen content in mouse tumors under ultrasound

[0037] The mice were divided into 5 groups, 3 mice in each group.

[0038] The mice received the following treatments:

[0039] (1) Control group: no treatment;

[0040] (2) The mice only inhaled oxygen group;

[0041] (3) The mice were added to the ultrasound group at the tumor site while inhaling oxygen;

[0042] (4) The group of mice injected with perfluorocarbon emulsion while inhaling oxygen;

[0043] (5) Mice were injected with perfluorocarbon emulsion, inhaled oxygen and added ultrasound group to the tumor site at the same time;

[0044] The ultrasonic addition time is 30 minutes. After the treatment of each group of mice, 0.8 mg / hypoxic fluorescent probe was injected intraperitoneally. After 90 minutes, the tumors of the mice were cut and frozen sections were stained. After the tumors were cut out, they were embedded with frozen section embedding agent and then ...

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Abstract

The invention relates to the technical field of biological medicine, in particular to a perfluorocarbon emulsion and a preparing method and application thereof, and particularly relates to an application to improving the oxygen content of tumors. The perfluorocarbon emulsion is prepared from perfluorocarbon and proteins, high oxygen carrying capacity is achieved, and oxygen can be rapidly released under the ultrasonic effect. An experiment indicates that the perfluorocarbon emulsion is injected into the caudal vein, a mouse is in the oxygen uptake state, the tumor site of the mouse is subjected to ultrasonic treatment, oxygen carried by the perfluorocarbon emulsion can be rapidly released under the mechanical effect of the ultrasonic treatment, and the content of oxygen at the tumor site is continuously increased. By means of the method, the oxygen-shortage condition at the tumor site can be effectively improved, and therefore the treatment effects of photodynamic therapy and radiotherapy are improved.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a perfluorocarbon emulsion and its preparation method and application, especially the application in increasing the oxygen content of tumors. Background technique [0002] Tumor refers to the neogrowth formed by the proliferation of local tissue cells in the body under the action of various tumorigenic factors, because this neogrowth is mostly in the form of space-occupying blocky protrusions, also known as neoplasm (neoplasm) . According to the cell characteristics of new organisms and the degree of harm to the body, tumors are divided into two categories: benign tumors and malignant tumors, and cancer is the general term for malignant tumors. Compared with benign tumors, malignant tumors grow faster and show invasive growth, are prone to bleeding, necrosis, ulcers, etc., and often have distant metastasis, resulting in emaciation, weakness, anemia, loss of appetite, fever, ...

Claims

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Application Information

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IPC IPC(8): A61K9/107A61K47/42A61K31/025A61P35/00A61N7/00A61M31/00
CPCA61K9/107A61K31/025A61K47/42A61M31/00A61M2202/0208A61N7/00A61M2202/0007
Inventor 刘庄宋雪娇
Owner JE & NA BIOTECH CO LTD
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