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Chenopodium quinoa saponin with enhanced antibacterial effect and preparation method thereof

A technology of quinoa saponin and antibacterial effect, which is applied in the field of quinoa saponin and its preparation, can solve problems such as no relevant reports, and achieve the effects of enhanced antibacterial effect, enhanced antibacterial effect, and enhanced antibacterial effect

Active Publication Date: 2016-12-07
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, studies on the components of saponins in quinoa bran have been reported. For example, quinoa saponins have immunomodulatory and weight-loss activities, but there are no relevant reports on the antibacterial effects of quinoa saponins, especially the research on the inhibition of oral bacteria.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of quinoa saponin with enhanced antibacterial effect, its preparation method is as follows:

[0030] (1) Quinoa bran is pulverized, passed through a 60-mesh sieve, 200 g is placed in a conical flask, 1600 mL of 70% methanol is added, stirred and extracted twice in a 45°C water bath, each time for 5 hours;

[0031] (2) Combine the two filtrates, rotary steam at a temperature of 50°C, and a vacuum of 0.1Mpa, and concentrate the extract to 80mL;

[0032] (3) Add 160 mL of ethyl acetate to the concentrated solution, shake and layer, remove the ethyl acetate layer, then add 160 mL of n-butanol, shake and layer, take the n-butanol layer and evaporate it in a water bath at 50°C to get quinoa crude saponins ;

[0033] (4) Prepare 1L of 5g / L quinoa crude saponin solution with distilled water, add 100mL of 1mol / L NaOH solution, stir and react at 90°C for 4 hours, cool to room temperature, add 0.1mol / L hydrochloric acid to adjust the pH to 7 ;

[0034] (5) Place the neut...

Embodiment 2

[0038] A kind of quinoa saponin with enhanced antibacterial effect, its preparation method is as follows:

[0039] (1) Quinoa bran is crushed, passed through a 60-mesh sieve, 400 g is taken and placed in a conical flask, 3200 mL of 70% methanol is added, stirred and extracted twice in a 65°C water bath, each time for 3 hours;

[0040] (2) Combine the two filtrates, rotate the evaporation temperature at 50°C, and vacuum at 0.1Mpa, and concentrate the extract to 150mL;

[0041] (3) Add 300mL of ethyl acetate to the concentrated solution, shake and layer, remove the ethyl acetate layer, then add 300mL n-butanol, shake and layer, take the n-butanol layer and evaporate it in a water bath at 50°C to get quinoa crude saponins ;

[0042] (4) Prepare 1 L of 10 g / L quinoa crude saponin solution with distilled water, add 200 mL of 1 mol / L NaOH solution, stir and react at 95°C for 2 hours, cool to room temperature, add 0.1 mol / L hydrochloric acid to adjust the pH to 7 ;

[0043] (5) Pl...

Embodiment 3

[0047] A kind of quinoa saponin with enhanced antibacterial effect, its preparation method is as follows:

[0048] (1) Quinoa bran is pulverized, passed through a 60-mesh sieve, 800g is taken and placed in a conical flask, 8000mL of 70% methanol is added, stirred and extracted twice in a 60°C water bath, each time for 4 hours;

[0049] (2) Combine the two filtrates, rotate the evaporation temperature at 50°C, and vacuum at 0.1Mpa, and concentrate the extract to 200mL;

[0050] (3) Add 400mL of ethyl acetate to the concentrated solution, shake and layer, remove the ethyl acetate layer, then add 400mL n-butanol, shake and layer, take the n-butanol layer and evaporate it in a water bath at 50°C to get quinoa crude saponins ;

[0051] (4) Prepare 1 L of 8 g / L quinoa crude saponin solution with distilled water, add 150 mL of 1 mol / L NaOH solution, stir and react at 92°C for 3 hours, cool to room temperature, add 0.1 mol / L hydrochloric acid to adjust the pH to 7 ;

[0052] (5) Pl...

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Abstract

The invention discloses Chenopodium quinoa saponin with enhanced antibacterial effect and a preparation method thereof. The preparation method comprises the steps of subjecting Chenopodium quinoa bran to 70% ethanol extraction, filtration and concentration for acquiring extract, cleaning with ethyl acetate and normal butanol, modification with alkaline solution, desalting, and purification with macroporous resin, and is finally lyophilized to obtain the Chenopodium quinoa saponin. It is possible to quickly and efficiently prepare the Chenopodium quinoa saponin with enhanced antibacterial effect. Bacterial culture experiments prove that the Chenopodium quinoa saponin treated with alkali is higher in antibacterial effect than the saponin not treated with alkali; particularly, in-vitro culture experiments via three oral pathogens (Porphyromonas gingivalis, Clostridium perfringens and Fusobacterium nucleatum) prove that the Chenopodium quinoa saponin treated with alkali is higher in antibacterial effect than the Chenopodium quinoa saponin not treated with alkali.

Description

technical field [0001] The invention relates to the technical field of extraction, in particular to a quinoa saponin with enhanced antibacterial effect, a preparation method and application thereof. Background technique [0002] Quinoa is a food crop native to South America with very high nutritional value. In recent years, there have been large-scale quinoa plantations in Shanxi, Jilin, Qinghai, Gansu, Hebei and other places in my country. Quinoa typically produces about 20%-30% quinoa bran during processing into quinoa rice. Quinoa bran contains about 2%-4% saponin, which makes quinoa bran taste bitter and cannot be directly used as animal feed. Studying the ingredients in quinoa bran, making full use of quinoa bran resources, and turning waste into wealth can produce significant economic value. [0003] Bad breath is usually caused by the sulfide produced by oral pathogenic bacteria in the oral cavity. According to statistics from the Chinese Stomatological Associatio...

Claims

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Application Information

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IPC IPC(8): A61K36/21A61P1/02A61P31/04
CPCA61K36/21A61K2236/333A61K2236/51A61K2236/55
Inventor 任贵兴杨修仕孙晓燕薛鹏
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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