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Novel Bacillus licheniformis strain and application thereof

A technology of Bacillus licheniformis and new strains, applied in the direction of application, bacteria, and chemicals for biological control, etc., can solve the problems of soil beneficial microbial flora destruction, insufficient supply of organic fertilizer, and affect the quality of agricultural products, etc., and reach the shelf life Long, solve the increasingly low soil fertility, improve the effect of soil fertility performance

Active Publication Date: 2016-12-07
廊坊市精耕天下农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the long-term application of chemical fertilizers, the soil beneficial microbial flora is damaged, the supply of organic fertilizer is insufficient, and the proportion of various nutrients is out of balance, resulting in different degrees of damage to the farmland ecological environment, soil physical and chemical properties and soil microbial flora. The current status of soil resources in my country urgently needs to solve the problem of soil being gradually destroyed through artificial measures to ensure the level of agricultural planting and the quality of crop products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The mycological characteristic of embodiment 1 bacillus licheniformis CLIMAX-VBL

[0028] Strain CLIMAX-VBL is isolated, purified, and rejuvenated from the root soil of crops. Its morphological characteristics are as follows: rod-shaped cells, positive Gram staining, and the size is (0.6-0.8 μm)×(2.0-3.5 μm). The spores are terminal or partial, cylindrical, and the cysts are not enlarged. On broth medium, the colonies are round, moist, with neat edges and red pigment.

[0029] Physiological and biochemical characteristics are as follows: positive reaction of contact enzyme, oxidase, amylase test; anaerobic growth; available propionate; negative reaction of lecithinase test.

Embodiment 2

[0030] The identification of embodiment 2 bacillus licheniformis CLIMAX-VBL

[0031] By determining the 16S rDNA sequence of the strain CLIMAX-VBL and comparing the sequencing results in GenBank, it was found that the sequence homology with other published 16S rDNA of Bacillus licheniformis was high.

[0032]Through the above morphological characteristics, physiological and ecological characteristics and 16S rDNA sequence analysis, it can be confirmed that the isolated new strain is indeed Bacillus licheniformis.

Embodiment 3

[0033] The preparation of embodiment 3 bacillus licheniformis CLIMAX-VBL bacterial agent (liquid fermentation mode)

[0034] Insert the strain CLIMAX-VBL stored in the lyophilized tube into the solid slant of the broth medium, and cultivate it at 32°C for 24 hours; then pick the colony and insert it into the PDA solid slant, and cultivate it at 32°C for 3- 7 days; then pick the colony and insert it into the sterilized liquid medium, cultivate it at 32°C and 150r / min for 3 days to obtain the fermentation broth of the strain CLIMAX-VBL, and use the fermentation broth directly as a liquid bacterial agent, Among them, the number of live bacteria of Bacillus licheniformis CLIMAX-VBL is more than 1 billion / g.

[0035] The liquid medium contains the following components in parts by weight: 20 parts of sucrose, 5 parts of sodium chloride, 20 parts of peptone, 5 parts of magnesium sulfate, 3 parts of potassium dihydrogen phosphate and 1000 parts of water.

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Abstract

The invention provides a novel Bacillus licheniformis strain CLIMAX-VBL of which the collection number is CGMCC No.12499. The invention also provides a microbial inoculant containing the Bacillus licheniformis CLIMAX-VBL and application thereof. The strain can obviously enhance the crop yield and stress tolerance, and obviously lowers the crop disease. The strain CLIMAX-VBL subjected to industrial production has wide application prospects, can be used for preparing multiple fertilizer products, and can be used for seedbeds, root irrigation, fertilizer preparation (manufacturing of biological organic fertilizers) and the like.

Description

technical field [0001] The invention relates to the fields of agricultural microbial preparations and soil fertilizers, in particular to a new strain of bacillus licheniformis and its application. Background technique [0002] White agriculture refers to the industrial new agriculture of microbial resource industrialization, and usually refers to the application of microbial fermentation engineering in agriculture. Agricultural production requires soil to provide nutrients for crop growth and a living environment. The application of chemical fertilizers has played a key role in increasing agricultural incomes for many years. However, due to the long-term application of chemical fertilizers, the soil beneficial microbial flora is damaged, the supply of organic fertilizer is insufficient, and the proportion of various nutrients is out of balance, resulting in different degrees of damage to the farmland ecological environment, soil physical and chemical properties and soil mic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N59/16A01N59/06A01P21/00C05G3/00C05G3/04C12R1/10C05G3/80
CPCA01N59/06A01N59/16A01N63/00C05F11/02C05G3/00C12N1/20C05G3/80C12N1/205C12R2001/10A01N2300/00C05F11/08
Inventor 刘澎
Owner 廊坊市精耕天下农业科技有限公司
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