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Phosphate-solubilizing potassium-solubilizing complex microbial agent special for tea trees and preparation method of phosphate-solubilizing potassium-solubilizing complex microbial agent

A compound bacterial agent and potassium solution technology, applied in the field of microorganisms, can solve problems such as poor effect and inability to meet the growth needs of tea trees in the north

Active Publication Date: 2016-12-14
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the Shandong tea area is an emerging tea area, and there are few special fertilizer products for tea trees. In particular, the tea tree microbial fertilizer market is almost blank, and most of the products sold are southern products. The effect is not good, and it cannot meet the needs of tea tree growth in the north.

Method used

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  • Phosphate-solubilizing potassium-solubilizing complex microbial agent special for tea trees and preparation method of phosphate-solubilizing potassium-solubilizing complex microbial agent
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  • Phosphate-solubilizing potassium-solubilizing complex microbial agent special for tea trees and preparation method of phosphate-solubilizing potassium-solubilizing complex microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1, the separation and identification of special potassium-solubilizing bacteria K2 for tea trees

[0014] The inventor collected soil samples from tea gardens in Shandong, placed them in sterile bags for low-temperature preservation, and brought them back to the laboratory for preservation at 4°C. Utilize potassium-solubilizing bacteria medium (yeast extract 0.5g, sucrose 10g, (NH 4 ) 2 SO 4 1.0g, Na 2 HPO 4 2.0g, MgSO 4 ·7H 2 O 0.5g, CaCO 3 1.0g, potassium feldspar powder 1.0g, dilute to 1000mL, adjust pH to 7.0-7.5) Screen target strains from soil. Combining traditional morphological, physiological and biochemical characteristics with PCR-based modern technology, the cultured strains were classified and identified, and finally a potassium-dissolving strain was screened.

[0015] According to the physiological and biochemical characteristics of the strain, it is found that the strain is Gram-positive, aerobic, positive for catalase and V-P, can us...

Embodiment 2

[0020] Example 2, the separation and identification of special inorganic phosphorus-decomposing bacteria WP2 for tea trees as an example

[0021] The inventors of the present invention collected soil samples from tea gardens in Shandong, placed them in sterile bags for low-temperature preservation, and brought them back to the laboratory for preservation at 4°C. Using inorganic phosphorus medium (glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O0.03g, MgSO 4 ·7H 2 O 0.3g, MnSO 4 4H 2 O 0.03g, yeast extract 0.4g, Ca 3 (PO 4 ) 2 10.0g, dilute to 1000mL with deionized water, adjust the pH value to 7.0-7.5) cultivate and screen the target strain from the soil. Combining traditional morphological, physiological and biochemical characteristics with PCR-based modern technology, the cultured strains were classified and identified, and finally an inorganic phosphorus decomposing bacterium was screened out.

[0022] According to morphological observat...

Embodiment 3

[0027] Embodiment 3, take the separation and identification of the special organophosphorus decomposing bacteria YP8 for tea tree as an example

[0028] The inventors of the present invention collected soil samples from tea gardens in Shandong, placed them in sterile bags for low-temperature preservation, and brought them back to the laboratory for preservation at 4°C. Using organic phosphorus medium (glucose 10g, (NH 4 ) 2 SO 4 0.5g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O0.03g, MgSO 4 ·7H 2 O 0.3g, MnSO 4 4H 2 O 0.03g, CaCO 3 5g, appropriate amount of lecithin, dilute to 1000mL, adjust the pH to 7.0-7.5) Screen the target strain from the soil. Combining traditional morphological, physiological and biochemical characteristics with PCR-based modern technology, the cultured strains were classified and identified, and finally an organophosphate-decomposing bacterium was screened out.

[0029] After morphological observation, it was found that the colony of the organophos...

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Abstract

The invention relates to a phosphate-solubilizing potassium-solubilizing complex microbial agent special for tea trees and a preparation method of the phosphate-solubilizing potassium-solubilizing complex microbial agent. The preparation method disclosed by the invention comprises the following steps: separating to obtain bacillus aryabhattai WP2, pseudomonas putida YP8 and potassium-solubilizing bacteria K2; inoculating the bacillus aryabhattai WP2, pseudomonas putida YP8 and potassium-solubilizing bacteria K2 into an LB fluid medium, thereby obtaining three kinds of liquid strain seeds; fully mixing the obtained three kinds of liquid strain seeds according to a volume ratio of 1:1:1, inoculating the seeds into a solid complex support, and culturing at a constant temperature of 35 DEG C for 24-48 hours until the viable count in the solid complex support reaches 4.0*10<10>cfu / g; drying the microbial agent meeting the viable count requirement at the temperature of 40 DEG C until the moisture content is less than or equal to 5%, thereby obtaining the special complex microbial agent special for tea trees. The special complex microbial agent disclosed by the invention has the phosphate-solubilizing and potassium-solubilizing effects. After the strain is prepared into a solid microbial agent, 2kg of the agent is applied to a tea garden per mu, so that the content of rapidly available phosphorus and rapidly available potassium in soil can be effectively improved, the one-hundred-bud weight of tea leaves can be improved, and the tea quality is improved.

Description

1. Technical field [0001] The invention relates to a phosphorus-dissolving and potassium-dissolving composite bacterial agent specially used for tea trees and a preparation method thereof, belonging to the technical field of microorganisms. 2. Background technology [0002] In recent decades, the large-scale application of chemical fertilizers has greatly increased the yield of tea leaves. However, for a long time, the single application of nitrogen fertilizers in tea gardens has caused the soil to deteriorate day by day. The taste of tea is weak, astringent and bitter, not resistant to brewing, and the quality is low. At the same time, the extensive use of chemical fertilizers has caused pollution to the environment and agricultural products. Therefore, people began to pay attention to the production of microbial fertilizers. Microbial fertilizer is a kind of active microorganism that increases the supply of plant nutrients through its life activities (including the supply...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/14A01N63/00A01P21/00C12R1/07C12R1/125C12R1/40
CPCA01N63/00C09K17/14C12N1/20
Inventor 韩晓阳张丽霞黄晓琴董玉慧周波
Owner SHANDONG AGRICULTURAL UNIVERSITY
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