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A latex-enhanced immune turbidimetric kit for detecting sod in serum

A technology of latex enhancement and immune turbidimetry, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that are not conducive to the health of testing personnel, confusion of measurement results, poor repeatability, etc.

Active Publication Date: 2018-04-03
CO HEALTH BEIJING LAB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the advantages of simple operation, rapidity, cheap reagents and small dosage; however, in practical application, this method is greatly affected by conditions such as reactant concentration, temperature, pH value, etc., thus causing confusion and lack of comparable results under different conditions. non-repeatable
Utilize in some detection reagent components of this method to contain toxic substance sodium cacodylate in addition, use this reagent for a long time, be unfavorable for the health of detection personnel

Method used

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  • A latex-enhanced immune turbidimetric kit for detecting sod in serum
  • A latex-enhanced immune turbidimetric kit for detecting sod in serum
  • A latex-enhanced immune turbidimetric kit for detecting sod in serum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of latex-enhanced immunoturbidimetric kit for detecting SOD in serum:

[0030] Prepare the components and concentrations of the invention kit reagent R1:

[0031]

[0032] Reagent R1 preparation method: 7.55g Na 2 HPO 4 .12H 2 O, 1.21g KH 2 PO 4 , 60g NaCl, 1g NaN 3 , 30g PEG6000, and 6ml calf serum were added to a beaker containing 500ml ultrapure water in sequence, placed on a magnetic stirrer and stirred at a low speed.

[0033] Prepare the reagent components and concentrations of the invention kit reagent R2:

[0034]

[0035] Reagent R2 preparation method: ① Preparation of buffer solution in reagent R2: take 12.1g Tris, 5g BSA, 1gNaN respectively 3, 0.001L Tween20 was added to 0.8L ultrapure water in turn, placed on a magnetic stirrer and stirred at low speed until completely dissolved, adjusted to pH 8.0, and added ultrapure water to make the volume to 1L; ②Preparation of allergenic latex in reagent R2: Add 640 μL of 155nm latex particles...

Embodiment 2

[0048] Embodiment 2: SOD kit performance evaluation

[0049] 1. Linear correlation

[0050] The kit B of the present invention prepared in Example 1 and the commercially available SOD detection kit A were used to detect 46 serum samples simultaneously, and the results of these 46 samples were analyzed by a linear regression equation to calculate the linear correlation of the two groups of kits. see results figure 1 (X, Y axes are measured values, unit U / ml), the results show that the correlation coefficient r=0.9983, the linear regression equation is: y=0.9985x+0.5904, according to the requirements of the American Clinical Laboratory Standardization Institute (CLSI) document (r >0.975), the SOD latex enhanced immune turbidimetry detection kit of the present invention has a good correlation with the commercially available SOD detection kit. The experimental data are shown in Table 1, and the regression equation is shown in the appendix figure 1 .

[0051] Table 1

[0052] ...

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Abstract

The invention discloses a latex-enhanced immunoturbidimetry kit for detecting SOD in serum. The kit comprises a reagent R1, a reagent R2 and a calibrator, the reagent R1 comprises a buffering solution, an electrolyte, a reaction enhancer, a stabilizer and a preservative, the reagent R2 comprises SOD antibody sensitization resistant latex particles, a buffering solution, an electrolyte, a stabilizer, a surfactant and a preservative, and the calibrator comprises a buffering solution, a stabilizer, a preservative and SOD recombinant protein. By utilizing polyclonal antibody to coat latex particles and combining the advantages of latex-enhanced immunoturbidimetry, the kit has the advantages of high sensitivity, high accuracy, wide linear range and high detection throughput and is highly suitable for clinical diagnosis application.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a kit for quantitatively detecting SOD in human serum by using latex-enhanced immune turbidimetry. Background technique [0002] Superoxide dismutase (superoxide dismutase, SOD) is a metalloenzyme widely present in organisms, and it is the primary substance for specifically scavenging free radicals in organisms. superoxide anion radical (O 2 -) is an important free radical produced by different reactions of the body. Under pathological conditions, excessive free radicals will cause damage to biological macromolecules such as DNA, protein and lipids, and cause various diseases in the body. [0003] SOD catalyzes superoxide anion radicals (O 2 -) undergoes a disproportionation reaction to eliminate the effects of superoxide anion free radicals (O-2) on the body. The level of SOD can reflect the content of free radicals in the body, and the content of the two in the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/573G01N33/544
CPCG01N33/544G01N33/573G01N2333/90283
Inventor 不公告发明人
Owner CO HEALTH BEIJING LAB