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Method for reducing ectopic pregnancy occurrence rate of test-tube baby patients by virtue of elective embryo transfer

A technology of embryo transfer and ectopic pregnancy, which is applied in the field of reproductive medicine science, can solve the problems of reducing the incidence of ectopic pregnancy, achieve the effect of reducing the incidence and maternal mortality, and reducing the incidence

Inactive Publication Date: 2017-02-01
黄官友
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, no studies have shown which method can reduce the incidence of ectopic pregnancy after IVF-ET

Method used

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  • Method for reducing ectopic pregnancy occurrence rate of test-tube baby patients by virtue of elective embryo transfer
  • Method for reducing ectopic pregnancy occurrence rate of test-tube baby patients by virtue of elective embryo transfer
  • Method for reducing ectopic pregnancy occurrence rate of test-tube baby patients by virtue of elective embryo transfer

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Experimental program
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Effect test

Embodiment approach

[0017] Implementation method, this method comprises the following steps:

[0018] (1) On the day of transplantation, the culture medium for culturing the embryos to be transplanted before each embryo transfer of each in vitro fertilization recipient is collected. On the first day after IVF, after fertilization was confirmed, each fertilized egg of each IVF recipient was placed in 30-50 μL containing 10% human serum albumin (SAGE In-Vitro Fertilization, Inc.; Trumbull , CT, USA) in the cleavage fluid (ART-1026, SAGE In-Vitro Fertilization, Inc.; Trumbull, CT, USA) droplet, set 37 ℃, 5% CO 2 Continue to culture in the incubator for 2 days until the embryo transfer; now, the embryos have been cultured in vitro to the 3rd day, 4h-6h before the embryo transfer, according to the morphological evaluation based on cleavage rate and morphology Evaluate the quality of the embryos to be transplanted for each IVF recipient, and each IVF recipient transfers 2-3 embryos at a time; after th...

Embodiment 1

[0026] 5126 (Medical record number) The name of the infertile woman, Liu XX, 27 years old, the name of the man, Lu XX, diagnosis: primary infertility: bilateral tubal obstruction after Lap operation; the woman retrieved eggs on January 18, 2014, and Embryo transfer was performed on January 21, 2014; before the embryo transfer, the concentration of IL-8 in the culture medium of the transferred embryo was determined to be 5.26pg / ml, and the grades of the transferred embryos were 1 / 6 and 1 / 7; 14 days after the embryo transfer operation, the blood HCG test was positive. On February 19, 2014, B-ultrasound: no gestational sac was seen in the uterus, but a gestational sac appeared in the right appendage, and the diagnosis was right tubal pregnancy; the operation was performed on February 29, 2014 Right salpingectomy.

Embodiment 2

[0028] 5130 (Medical record number) Infertile couple, woman's name, Liu X, 37 years old, man's name, Rao X, diagnosis: secondary infertility: bilateral tubal obstruction; bilateral tubal pregnancy after conservative surgery; endometrial polyps hysteroscopy Postoperatively; the woman took eggs on January 21, 2014, and performed embryo transfer on January 24, 2014; before the embryo transfer, the concentration of IL-8 in the culture medium of the transplanted embryo was determined to be 0.27pg / ml, The grades of the transplanted embryos were 1 / 8 and 1 / 8; the woman's blood HCG test was positive 14 days after the embryo transfer operation, and on February 28, 2014, B-ultrasound: a 75*63mm uneven echo mass in the right accessory area was diagnosed as Right tubal pregnancy; On March 3, 2014, the right tubal incision and embryo extraction were performed.

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Abstract

The invention discloses a method for reducing an ectopic pregnancy occurrence rate of test-tube baby patients by virtue of elective embryo transfer. The method comprises the following steps: detecting the content of interleukin-8 (IL-8) in a specimen by using a liquid chip high-throughput protein analysis technique (LHTPA); performing elective embryo transfer according to a morphological evaluation method and the content of the interleukin-8 (IL-8) in the specimen, and transferring an embryo of which the IL-8 is negative in the culture solution, that is, the IL-8 concentration in the culture solution is less than 0.19pg / ml; screening to-be-transferred embryos for transferring on the same day of embryo transfer by using the method if the operating time of collecting the specimen and detecting the IL-8 is 4-6 hours, wherein the ectopic pregnancy occurrence rate after embryo transfer by using the negative IL-8 in the human embryo culture solution before transfer is 1.10%, while the ectopic pregnancy occurrence rate after embryo transfer by using positive IL-8 is 10.34%. Therefore, the elective embryo transfer is performed by using the method, so that the ectopic pregnancy occurrence rate can be obviously reduced, and an occurrence rate of maternal complications during early pregnancy and a maternal death rate are further reduced.

Description

technical field [0001] The invention belongs to the field of reproductive medicine science and test-tube baby transplantation, and specifically relates to the selection of interleukin-8 (IL-8) by measuring the content of interleukin-8 (IL-8) in the culture medium for culturing embryos to be transplanted before human embryo transplantation before test-tube baby transplantation. Embryo transfer to reduce the incidence of ectopic pregnancy (ectopic pregnancy). Background technique [0002] Ectopic pregnancy is the leading cause of maternal complications in the first trimester and the leading cause of maternal death. In developed countries, women who die from ectopic pregnancy account for 5% of maternal deaths, and in developing countries, women who die from ectopic pregnancy account for 10% of maternal deaths. For the normal population, the incidence of ectopic pregnancy rate (ectopic pregnancy rate) (number of ectopic pregnancy / total number of pregnancy) is 1-2%, while for pa...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6869G01N2333/5421
Inventor 黄官友
Owner 黄官友
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