86 results about "Interleukin 8" patented technology

This invention relates to a method for the biochemical treatment of persistent pain disorders by inhibiting the biochemical mediators of inflammation in a subject comprising administering to said subject any one of several combinations of components that are inhibitors of biochemical mediators of inflammation. Said process for biochemical treatment of persistent pain disorders is based on Sota Omoigui's Law, which states: ‘The origin of all pain is inflammation and the inflammatory response’. Sota Omoigui's Law of Pain unifies all pain syndromes as sharing a common origin of inflammation and the inflammatory response. The various biochemical mediators of inflammation are present in differing amounts in all pain syndromes and are responsible for the pain experience. Classification and treatment of pain syndromes should depend on the complex inflammatory profile. A variety of mediators are generated by tissue injury and inflammation. These include substances produced by damaged tissue, substances of vascular origin as well as substances released by nerve fibers themselves, sympathetic fibers and various immune cells. Biochemical mediators of inflammation that are targeted for inhibition include but are not limited to: prostaglandin, nitric oxide, tumor necrosis factor alpha, interleukin 1-alpha, interleukin 1-beta, interleukin-4, Interleukin-6 and interleukin-8, histamine and serotonin, substance P, Matrix Metallo-Proteinase, calcitonin gene-related peptide, vasoactive intestinal peptide as well as the potent inflammatory mediator peptide proteins neurokinin A, bradykinin, kallidin and T-kinin.

The invention provides methods, oral care products and kits for treating mouth tissues of an animal. In particular, the invention provides methods, oral care products and kits which use or comprise a non-peptide polyamine chelating agent, most preferably trientine, or a physiologically-acceptable salt thereof, which can inhibit the release of pro-inflammatory cytokines, particularly interleukin 8, from cells located in tissues of the mouth and can reduce the damage done by reactive oxygen species (ROS) to such tissues.

Isolated human monoclonal antibodies which bind to IL-8 (e.g., human IL-8) are disclosed. The human antibodies can be produced in a hybridoma, transfectoma or in a non-human transgenic animal, e.g., a transgenic mouse, capable of producing multiple isotypes of human monoclonal antibodies by undergoing V-D-J recombination and isotype switching. Also disclosed are pharmaceutical compositions comprising the human antibodies, non-human transgenic animals, hybridomas, and transfectomas which produce the human antibodies, and therapeutic and diagnostic methods for using the human antibodies.

The present invention relates to pharmaceutical kits and methods to treat lung inflammation and redox imbalance in human cystic fibrosis patients using pharmaceutical compositions containing N-acetylcysteine (NAC), pharmaceutically acceptable salts of N-acetylcysteine, or N-acetylcysteine derivatives. In phase I studies, treatment with oral NAC at a dose of from about 1800 mg/day to about 3000 mg/day for a period of 4 weeks produced significant positive effects, namely, it decreased absolute numbers of white blood cells and neutrophils in the sputum and produced concomitant decreases in sputum neutrophil elastase specific activity and sputum interleukin-8 levels, suggesting an amelioration of lung inflammation in the patients. These effects were associated with an increased total GSH level in whole blood as well increased staining for reduced GSH in blood neutrophils, both of which reflect an amelioration of the redox imbalance in the patients. In ongoing phase II studies, oral NAC at a dose of about 2700 mg/day administered in double-blind manner for 12 weeks showed excellent safety and significantly decreased white blood cells in sputum as compared to placebo.

The present embodiments are related to high-affinity antibodies directed to IL-8, methods of making and characterizing such antibodies and uses of such antibodies. Isolated polynucleotide sequences encoding, and amino acid sequences comprising, heavy and light chain immunoglobulin molecules, particularly sequences corresponding to contiguous heavy and light chain sequences spanning the framework regions (FR's) and/or complementarity determining regions (CDR's), are provided.

Disclosed herein are methods for diagnosing or treating pregnancy related hypertensive disorders that include the use of a polypeptide or a nucleic acid encoding a polypeptide selected from the following: follistatin related protein, interleukin 8, inhibin A, VEGF-C, angiogenin, beta fertilin, hypothetical protein, leukocyte associated Ig-like receptor secreted protein, erythroid differentiation protein, adipogenesis inhibitory factor, corticotropin releasing factor binding protein, alpha-1 anti-chymotrypsin, insulin-like growth factor binding protein-5, CD33L, cytokine receptor like factor 1, platelet derived endothelial growth factor, lysyl hydroxylase isoform 2, stanniocalcin precursor, secreted frizzled related protein, galectin-3, alpha defensin, ADAM-TS3, cholecystokinin precursor, interferon stimulated T-cell alpha chemoattractant precursor, azurocidin, sperminine oxidase, UDP glycosyltransferase 2 family polypeptide B28, neurotrophic tyrosine kinase receptor 2, neutral endopeptidase, CDC28 protein kinase regulatory subunit 2, beta glucosidase, lanosterol synthase, calcium/calmodulin-dependent serine protein kinase, estrogen receptor-alternatively spliced transcript H, chemokine (CX3C motif) receptor 1, tyrosinase-related protein 1, hydoxy-delta-5-steroid dehyrogenase, dihydropyramidinase-like-4, and cytochrome P450-family 11.

The invention relates to methods and kits for detecting the likelihood that a subject has cancer, e.g., squamous cell carcinoma, by assaying the expression levels of tumor associated genes. More specifically, the expression levels of nucleic acids or proteins can be assayed in the tumor associated genes, e.g., over-expression of beta-2 microgobulin (B2M), keratin 17 (KRT17), interleukin 8 (IL8), or annexin A2 (ANXA2), and under-expression of cytochrome p450 1B1 (CYP1B1) or laminin gamma-2 (LAMC2) can be indicative of the likelihood a subject has squamous cell carcinoma or a precancerous squamous cell disorder. The expression levels compared to standards can be indicative of the likelihood a subject has squamous cell carcinoma. The expression levels of B2M, CYP1B1, KRT17, IL8, ANXA2, or LAMC2 can also be repeatedly assayed to monitor the progression of a squamous cell neoplasia.

The present invention reports stimulatory effects of interleukin 8 (IL-8) on human osteoclast formation and bone resorption, indicating IL-8 as a potent activator of bone destruction common in metastatic bone diseases. Tumor growth and osteolysis were inhibited by anti-IL-8 antibody or antisense IL-8. Additionally, IL-8 was able to confer an osteolytic phenotype on non-osteolytic cancer cells. These results identify tumor-induced osteolysis and bone resorption as potential targets of anti-IL-8 therapy.

A method for predicting treatment response of a type II diabetes patient to rosiglitazone is provided. The method involves at least one sample from a patient having type II diabetes and analyzing biomarkers predictive of a patient who will respond to treatment with rosiglitazone. The biomarkers include, at least, interleukin-8, histidine, citrate. These biomarkers are identified in at least one classification analyses selected from the group consisting of a majority-vote based classifier and support-vector machine (SVM) classifier. Also provided is a method for predicting treatment response of a type II diabetes patient to glyburide at 8 weeks post-initiation of therapy. The method involves obtaining a sample from a type II diabetes patient who has been treated with glyburide for about 4 weeks and analyzing biomarkers predictive of a patient who will respond to treatment with glyburide at 8 weeks. The biomarkers useful in this method include, at least, sphingomyelin 23:1 and L-phenylalanine. Also provided are kits useful for the methods of the invention.

The present invention provides a topical preparation for treating patients with skin inflammatory disease. The topical preparation contains an antibody to interleukin-8 (IL-8) and a pharmaceutically acceptable carrier. The antibody can be a monoclonal antibody, a polyclonal antibody, an antibody fragment, or a combination thereof. The monoclonal antibody is a murine anti-human IL-8 monoclonal antibody produced in a hybridoma. The polyclonal antibody is a chicken anti-human polyclonal antibody, which is prepared by immunizing a chicken with human IL-8, collecting eggs from the immunized chicken, and purifying the IgY from the eggs. The topical preparation is effective in topically treating inflammatory skin diseases. The present invention also provides a method for treating patients with skin inflammatory disease, which is by topically applying an effective amount of the topical preparation onto patients with skin inflammatory disease.

There is provided an in vitro assay for screening a test compound for toxicity in renal proximal tubular cells. The method comprises contacting a test compound with a test population of renal proximal tubular cells; and determining the expression level of an interleukin in the test population, the interleukin being interleukin-6 (IL-6) or interleukin-8 (IL-8), or both. Expression levels of the interleukin in the test population being greater than expression levels in a control population of renal proximal tubular cells not contacted with the test compound is indicative that the test compound is toxic for renal proximal tubular cells.

PCT No. PCT/JP96/02412 Sec. 371 Date Jun. 5, 1997 Sec. 102(e) Date Jun. 5, 1997 PCT Filed Aug. 28, 1996 PCT Pub. No. WO97/07813 PCT Pub. Date Mar. 6, 1997Leukocyte chemotactic factors such as interleukin-8 and MCAF, and inductive substances therefor were revealed to have proconceptive activities. These activities include promoting ovum growth and fertilized ovum implantation, and are exhibited by a sole substance. Accordingly, it has been shown that such substances can be used in drugs concerning medical treatment for infertility, and further, can be used in veterinary and livestock industry fields such as reproduction of industrial animals or species preservation of rare animals.

The invention discloses an application of mi-RNA (micro-ribonucleic acid) with an AAAGUGC seed sequence in preparing an IL8 (interleukin 8) inhibitor. The mi-RNA with the AAAGUGC seed sequence can directly target the 3' non-coding region of the mRNA of IL8, so that secretion of IL8 can be effectively inhibited, and generation of cell endogenic IL8 can be reduced. Therefore, the mi-RNA with the AAAGUGC seed sequence can be used as a cell endogenic IL8 generation inhibitor and used for preventing or treating various diseases or disease states caused by IL8 generation or increase.

The invention discloses a composition for promoting physiologically regulative regeneration of a damaged tissue. The composition comprises such components as albumin, epidermal growth factor, transforming growth factor alpha, keratinocyte growth factor, basic fibroblast growth factor, platelet-derived growth factor, vascular endothelial growth factor, interleukin-8 and granulocyte-macrophage colony stimulating factor. The eight protein factors are combined for use to comprehensively develop the physiological tissue repair function; the eight protein factors functionally promote each other; based on the own physiological tissue recovery mechanism of the human body, the wound healing is accelerated and the wound healing quality is improved, including recovering the structure and the functions of the normal skin tissue. Each cell factor-containing component has clear mechanism of action without any potential toxic and side effect and objectionable odor. Such a combined recovery agent is capable of recovering the normal structure and the physiological functions of tissues more quickly under the physiological conditions of the human body.

The invention provides a recombinant serum I type MDV (Marek's Disease Virus) (a BZ-1 strain). The recombinant serum I type MDV is actually characterized in that pathogenic associated genes (meq and vIL-8 (Viral Interleukin 8) genes) of an isolated MDV virulent strain BJ07 are knocked out at the same time, and a constructed MDV gene deleted strain (the BZ-1 strain) has no pathogenicity, cannot induce tumor of chicken and has no immunosuppressive action on chicken flocks; the BZ-1 strain is used as a vaccine prepared by a strain produced by MD (Marek's Disease) live vaccine, chicken MD induced by a very virulent MDV can be prevented, and the protective immune effect is superior to that of a CVI988/Rispens strain vaccine which is most widely applied in domestic and foreign markets at present.

Described are methods of treating or preventing cancer in patients by administering Interleukin 6 (IL-6) inhibitor and Interleukin 8 (IL-8) inhibitor, in a concentration ratio range to inhibit the migration of cancer cells.

The invention discloses a method for screening probiotics. The method comprises the following steps of: (1) carrying out a primary culture on spleen tissues of a pig or a domestic animal; (2) attacking toxicity of the tissues through colon bacillus K88, and testing variations of levels of an interleukin 8 and an interleukin 4; (3) respectively processing the tissues through candidate probiotic strains, and testing variations of levels of two interleukins; (4) comparing the variations to select probiotic strains which are similar in variation degrees of the two interleukins but opposite in effect due to treatment of the colon bacillus K88; and (5) through the colon bacillus K88 animal toxicity attacking test, observing protecting effects of primarily selected probiotic strains, and finally determining an efficient probiotic strain. The method for screening the probiotic disclosed by the invention has the remarkable advantages that: the method combining in vitro immunology experiment and in vivo toxicity attacking experiment is high in accuracy, strong in pertinency and time-saving; and the screened probiotics can effectively replace antibiotic, and greatly reduce diseases caused by colon bacillus so as to promote the health of the animal.