A kind of liquid curdlan gum and its production method
A production method and liquid technology, which are applied in the field of liquid keratin and its production, can solve the problems of troublesome rehydration steps, dust pollution, product loss, etc., so as to prolong the storage time, avoid potential safety hazards and have good storage stability. Effect
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Embodiment 1
[0045] The preparation of curdlan gum fermentation liquid uses a microbial fermentation method, which can be routinely obtained by those skilled in the art, as follows: using Alcaligenes faecalis as the starting strain, use an inoculation loop to pick a few well-growing colonies and inoculate them on the slope culture medium at 30°C for 3 days. The activated strains were then inserted into the seed medium, cultivated at 30°C for 20 hours, added to the fermentation medium according to the inoculum size of 2.5%, and cultivated at 30°C for 72 hours to obtain curdlan gum fermentation liquid, which was passed through The following steps are processed to get the final product, the process is as follows image 3 shown.
[0046] (1) Alkali dissolution: take curdlan gum fermentation broth, dilute the yield to 1.8%-2.5% (w / w) with water to obtain curdlan gum diluent, then add the curdlan gum diluent mass 1.5% sodium hydroxide, stirring continuously to make it completely dissolved, to ...
Embodiment 2
[0058] The preparation of curdlan gum fermentation liquid uses a microbial fermentation method, which can be routinely obtained by those skilled in the art, as follows: using Alcaligenes faecalis as the starting strain, use an inoculation loop to pick a few well-growing colonies and inoculate them on the slope culture medium at 30°C for 3 days. The activated strains were then inserted into the seed medium, cultivated at 30°C for 20 hours, added to the fermentation medium according to the inoculum size of 2.5%, and cultivated at 30°C for 72 hours to obtain curdlan gum fermentation liquid, which was passed through The following steps process the final product.
[0059] (1) Alkali dissolution: take curdlan fermentation broth, add water to dilute the gum content to 1.8%-2.0%, then add 1.5% liquid alkali, stir continuously to dissolve completely, and obtain a viscous and uniform alkali solution.
[0060] (2) Sedimentation and impurity removal: add flocculant to the alkali solution...
Embodiment 3
[0070] The determination of the absorbance A value of embodiment 3 sedimentation supernatant
[0071] The method is the same as in Example 1, except that the settling time and the absorbance A value are different.
[0072] The curdlan gum fermentation broth is diluted with alkali and then added flocculant to settle. We determine the end point of the settlement by measuring the absorbance A value of the alkali-dissolved supernatant, so as to ensure the uniformity of product extraction and the stability of product indicators. Specifically by measuring the OD at different settling times 600 A value to determine gel strength, ash content (GB5009.4), total nitrogen (trace Kjeldahl method GB / T609), curdlan content (based on anhydrous glucose) (GB28304-2012); The powder prepared in Example 1 in No. 201410089577.4 is used as a control, and the specific data are shown in Table 3, figure 1 .
[0073] Table 3 Settlement value and product index
[0074]
[0075] From Table 3 and f...
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