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Trichoderma asperellum and application thereof in remediation for heavy metal pollution

A technology for Trichoderma aculeatus and heavy metals is applied to Trichoderma aculeatus and its application field in heavy metal pollution remediation, and can solve problems such as affecting the subsequent utilization of soil, adverse effects of soil physical and chemical properties, and remediation of polluted soil.

Inactive Publication Date: 2017-02-22
HUNAN AGRI BIOTECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional physical and chemical remediation methods are effective but require a large amount of engineering, consume a large amount of energy or chemicals, etc., and have high costs. The use of some passivators may even cause remediation of polluted soil and have adverse effects on soil physical and chemical properties, thereby Affect the subsequent use of soil

Method used

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  • Trichoderma asperellum and application thereof in remediation for heavy metal pollution
  • Trichoderma asperellum and application thereof in remediation for heavy metal pollution
  • Trichoderma asperellum and application thereof in remediation for heavy metal pollution

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Isolation and identification of embodiment 1 bacterial strain HD228

[0029] 1. Isolation of strains

[0030]The soil samples were collected from Hengdong County, Hunan Province, which belongs to the paddy fields in the Xiangjiang River Basin. The sampling point was located with a GIS map tool (Garmin) to collect 2kg of 5-20cm soil in the late paddy field of one season. After mixing, it was divided into two sterile sealed bags, one bag Store in an ice box at 4°C, and air-dry one bag to measure the soil heavy metal content and pH value. The contents of Cd, Cu, Zn, and Pb in the soil samples were 32.09mg / kg, 30.9mg / kg, 347.9mg / kg, and 86.0mg / kg, respectively, and the soil pH value was 4.7.

[0031] Take 10 mg of soil stored at 4°C and dissolve it in 90 mL of sterile deionized water, and stir magnetically for 30 min to prepare a soil suspension. Take 1 mL of the soil suspension and inoculate it in a solution containing 2 mM Cd(NO 3 ) 2 Streptomycin (30mg / L) was added t...

Embodiment 2

[0039] Example 2 The adsorption capacity test of cadmium-resistant bacterial strain HD228 to cadmium

[0040] will not contain Cd 2+ The HD228 bacteria grown on the plate for 7 days were scraped into without Cd 2+ In the PDA liquid culture medium, 150rpm shaker was cultured at 28°C for 7 days, and then 8000rpm was centrifuged for 15min, and 0.1g, 0.5g, 1g, 1.5g were weighed under sterile conditions (the dry weight of mycelia, 1g of wet mycelia Place the mycelia in a 60°C oven to dry until the mycelium quality is constant, and calculate the dry weight of the wet mycelium) Mycelia were inoculated in 100mg / L and 200mg / L Cd-containing 2+ In the PDA liquid medium, in order to avoid the influence of the medium on Cd 2+ The adsorption of 100mg / L and 200mg / L without mycelium was used as the control respectively, and the Cd in the supernatant was determined by atomic absorption spectrophotometry after being cultured at 28°C for 7 days at 150rpm and centrifuged at 8000rpm for 15min. ...

Embodiment 3

[0045] Example 3 Bacterial strain HD228 cadmium tolerance and other heavy metal tolerance tests

[0046] Take 16mM Cd 2+ The HD228 mycelia isolated on the plate were successively inoculated in 20mM, 25mM, 30mM, 35mM, 40mM, 45mM and other Cd 2+ Concentration of the PDA plate, cultured at 28°C for 7 days, until the colonies do not grow, the Cd 2+ The concentration is the MIC value of Cd to HD228; use a 5mm diameter puncher to punch out a plate of bacteria on a PDA plate with a single colony of HD228, and inoculate it on a plate containing Pb(NO 3 ) 2 (0, 5mM, 10mM, 20mM, 40mM, 80mM), ZnSO 4 (0, 10mM, 20mM, 40mM, 80mM), CuSO 4 (0, 5mM, 10mM, 20mM, 30mM) on a PDA plate, cultured at 28°C for 7 days, and measured the colony diameter with the straightedge cross method. The diameter of the colony on the plate can reflect the biomass of the fungal growth. The relationship between the biomass of colonies and the concentration of other heavy metal ions common in cadmium-contaminated...

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Abstract

The invention relates to trichoderma asperellum and an application thereof in remediation for heavy metal pollution. The trichoderma asperellum HD228 is separated from cadmium-containing soil in Hengdong County, Hunan, and the preservation number of the trichoderma asperellum HD228 is CGMCC No.12870. The strain (the trichoderma asperellum HD228) has a high cadmium adsorbing capacity, and meanwhile, the strain has a growth promoting effect on rice and has a detoxifying effect on cadmium-stressed rice. Therefore, the trichoderma asperellum HD228 has a potential of developing double functions in a process of conducting soil remediation on cadmium-contaminated rice fields, and a good strain is provided for cadmium-contaminated soil microbial remediation engineering. The application provided by the invention has theoretical and realistic significance for the remediation of cadmium pollution of the cadmium-contaminated rice fields.

Description

technical field [0001] The invention relates to the technical field of microbiology and ecological restoration, in particular to a trichoderma aculeatus and its application in heavy metal pollution restoration. Background technique [0002] In 2012, it was reported that the land seriously polluted by cadmium in the country had exceeded 13,300 hm 2 . Hunan is an area severely affected by cadmium pollution. Sampling and testing found that the cadmium content in fields near the Dapu Town Industrial Park in Hengdong, Hunan even reached 130mg / kg, which is 433 times the value of the secondary standard for soil environmental quality, while Youxian, Xiangyin, Changsha, Changning Paddy fields in some areas such as China have also been polluted by cadmium to varying degrees. The main direct source of cadmium pollution in rice is soil, and the task of remediation of cadmium-contaminated paddy soil is becoming more and more urgent. The remediation methods of cadmium-contaminated padd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14B09C1/10A01N63/04A01P21/00C12R1/885
CPCA01N63/30B09C1/105C12N1/145C12R2001/885
Inventor 邓亚男柏连阳罗坤周小毛彭迪郑景泉蒋慧丹邬腊梅王立峰
Owner HUNAN AGRI BIOTECH RES CENT
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