Bacillus licheniformis, microbial inoculum, application thereof and method for passivating heavy metals
A technology of Bacillus licheniformis and heavy metals, which is applied in the restoration of polluted soil, bacteria and other directions, can solve the problems of long restoration period, single restoration, high energy consumption, etc., and achieves high economic and social benefits, easy operation, and energy consumption. small effect
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[0054] This embodiment is used to illustrate the passivation performance of Bacillus licheniformis of the present invention to heavy metals
[0055] (1) Passivation ability for heavy metals in aqueous phase
[0056] Add heavy metals cadmium, arsenic, lead, mercury and chromium to the deionized water in such an amount that the concentration of each heavy metal is 100mg / L, sterilize at 121°C for 15min, and cool to room temperature for later use.
[0057] Insert the above-activated bacterial strain LH-R of the present invention into LB liquid medium according to the inoculation amount of 1-2%, shake and cultivate at 28-30°C and 170rpm for 18h, take 100mL of cultured bacterial liquid at 4°C, 4000rpm Centrifuge for 20 minutes, collect all the bacteria, add the bacteria to the aqueous solution containing heavy metals cadmium, arsenic, lead, mercury, and chromium prepared above, and incubate at 170 rpm at 30±1°C for 8 hours. Then centrifuge sampling, detect the content of cadmium, a...
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