Bacillus licheniformis, microbial inoculum, application thereof and method for passivating heavy metals

A technology of Bacillus licheniformis and heavy metals, which is applied in the restoration of polluted soil, bacteria and other directions, can solve the problems of long restoration period, single restoration, high energy consumption, etc., and achieves high economic and social benefits, easy operation, and energy consumption. small effect

Active Publication Date: 2017-03-15
粮华生物科技(北京)有限公司
View PDF1 Cites 24 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the physical method often requires a large amount of engineering and consumes a lot of energy; most of the chemical methods are expensive and easy to cause secondary pollution; phytoremediation in the biological method is favored by many researchers, but the plants grow slowly and the repair period is lo

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus licheniformis, microbial inoculum, application thereof and method for passivating heavy metals
  • Bacillus licheniformis, microbial inoculum, application thereof and method for passivating heavy metals
  • Bacillus licheniformis, microbial inoculum, application thereof and method for passivating heavy metals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] This embodiment is used to illustrate the passivation performance of Bacillus licheniformis of the present invention to heavy metals

[0055] (1) Passivation ability for heavy metals in aqueous phase

[0056] Add heavy metals cadmium, arsenic, lead, mercury and chromium to the deionized water in such an amount that the concentration of each heavy metal is 100mg / L, sterilize at 121°C for 15min, and cool to room temperature for later use.

[0057] Insert the above-activated bacterial strain LH-R of the present invention into LB liquid medium according to the inoculation amount of 1-2%, shake and cultivate at 28-30°C and 170rpm for 18h, take 100mL of cultured bacterial liquid at 4°C, 4000rpm Centrifuge for 20 minutes, collect all the bacteria, add the bacteria to the aqueous solution containing heavy metals cadmium, arsenic, lead, mercury, and chromium prepared above, and incubate at 170 rpm at 30±1°C for 8 hours. Then centrifuge sampling, detect the content of cadmium, a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of microbial environment repair, and specifically discloses bacillus licheniformis and an application thereof. The collection number of the bacillus licheniformis is CGMCCNo.10852. The invention further discloses a microbial inoculum containing the bacillus licheniformis and a method for passivating heavy metals. The bacillus licheniformis disclosed by the invention is capable of playing a passivating role on many heavy metals (cadmium, arsenic, lead, mercury and chromium) in a water body and soil simultaneously. The removal rates of the bacillus licheniformis provided by the invention for cadmium, arsenic, lead, mercury and chromium are up to 73.49%, 68.26%, 81.07%, 58.44% and 70.21% respectively in a water phase. The bacillus licheniformis is also capable of effectively passivating the abovementioned five heavy metals in soil with heavy metal pollution. Moreover, five heavy metals can be passivated simultaneously through adopting the bacillus licheniformis provided by the invention, the operation is simple and convenient, the energy consumption is low, secondary pollution is avoided, the period is short, and high economic benefits and social benefits are achieved.

Description

technical field [0001] The invention relates to the field of microbial environment restoration, in particular to a strain of Bacillus licheniformis with the function of passivating heavy metals, a bacterial agent containing the Bacillus licheniformis, their application and a method for passivating heavy metals. Background technique [0002] With the intensification of industrial and urban pollution and the increase in the types and quantities of agricultural chemicals, soil heavy metal pollution is becoming more and more serious, and the polluted area is also expanding year by year. Heavy metal pollutants stay in the soil for a long time, cannot be degraded by microorganisms, and can eventually affect human health through media such as water and plants. my country's soil is seriously polluted by heavy metals such as cadmium, arsenic, lead, mercury, and chromium. According to the national survey of sewage irrigation areas conducted by the Ministry of Agriculture of my country...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): B09C1/10C12N1/20
Inventor 林森徐粲然任立伟王培红
Owner 粮华生物科技(北京)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap