Culture method of adipose tissue-derived stromal cells

A culture method and stem cell technology, applied in the direction of cell culture active agent, tissue culture, animal cells, etc., can solve the problems of poor mobility, limited expansion ability, etc., to solve the limited expansion ability, improve the expansion ability, high The effect of migratory ability
CN106520686AInactive Publication Date: 2017-03-22海南博鳌一龄迈迪精准医学研究院有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
海南博鳌一龄迈迪精准医学研究院有限公司
Publication Date
2017-03-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a culture method of adipose tissue-derived stromal cells. The culture method of the adipose tissue-derived stromal cells comprises the following culture step: inoculating acquired primary-generation adipose tissue-derived stromal cells into a stem cell culture medium, wherein the stem cell culture medium contains stem cell factors and interleukin-3 factors, and the content ratio of the stem cell factors to the interleukin-3 factors is (5 to 20 [mu]mol / L) to (5 to 20 [mu]mol / L). The culture method can improve the multiplication ability of the adipose tissue-derived stromal cells and achieve relatively high transfer ability.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for culturing adipose-derived mesenchymal stem cells. Background technique

[0002] Mesenchymal stem cells (Mesenchymal Stem Cells, MSCs) were first discovered in the bone marrow and have been confirmed to exist in various tissues and organs of the body. They are a group of pluripotent stem cells with multi-directional differentiation potential. Compared with MSCs from other sources, adipose derived stromal cells (ADSCs) have great advantages in isolation and culture:

[0003] (1) It is convenient to obtain materials. Adipose tissue reserves are abundant, and it is less painful to obtain samples. At present, 200ml of fat can be obtained in one liposuction operation, and about 1×10 6 stem cells, which is 40 times that of bone marrow; (2) The separation is simple. ADSCs can be obtained from peripheral fat by mechanical shearing, collagenase digestion and simple...

Claims

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