Compound typing system used for personal identification
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A technology of genetic markers and loci, applied in the field of forensic identification, can solve problems such as unsatisfactory typing and identification results, and inability to make satisfactory family identification conclusions
Active Publication Date: 2017-03-22
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA +1
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[0005] Although many genetic marker typing systems for identification and individual identification have been developed, for some relatives with distant ancestry, the existing genetic marker composite typing system cannot make a satisfactory conclusion on family identity
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Embodiment 1 1
[0053] Example 1 A group of genetic marker combinations
[0054] The genetic marker combination includes 482 SNP sites described in the present invention, and the rs numbers and genetic information of the 482 SNP sites are shown in Table 1.
[0059] A random sample experiment and result analysis were performed on family members with 16 relatives.
[0060] Individual samples: a total of 47 family members of 16 relatives, including parents, children, brothers, sisters, cousins, grandparents and nephews; blood samples were used as samples.
[0061] experiment procedure:
[0062] Venous blood collection was performed on the above 47 individuals, and 47 blood samples were obtained, which were randomly numbered and double-blind experiments were carried out:
[0063] (1), DNA extraction:
[0064] Use GenElute TM The blood genomic DNA kit extracted genomic DNA from 47 blood samples respectively, and obtained 47 blood genomic DNA samples. For the specific extraction process, refer to the kit instructions;
[0065] (2), library preparation:
[0066] A. PCR amplification:
[0067] Using the 47 blood genomic DNA samples obtained in step (1) of this embodiment as PCR templates, primers were designed, and Ion AmpliSeq was u...
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Abstract
The invention aims at providing a compound typing system used for personal identification, which is used for personal identification, especially the identification for relative relations such as grandparents and grandchild, uncles and nephews, or half sibs, and has the powerful typing efficiency and wide application range. On one aspect, the invention provides a set of genetic marker assembly, and the genetic marker assembly comprises 482 SNP sites positioned on the autosome; on another aspect, the invention provides a typing kit, and the typing kit comprises a reagent for typing the 482 SNP sites in the genetic marker assembly provided by the invention; on still another aspect, the invention provides an individual genotype identity card, and the individual genotype identity card comprises a chip, the genotype information of the genetic marker sites of the individuals is stored in the chip, and the genetic marker sites refer to the 482 SNP sites in the genetic marker assembly provided by the invention.
Description
technical field [0001] The invention belongs to the technical field of forensic identification and relates to a compound typing system for identification, in particular to 482 SNP sites and its application in identification. Background technique [0002] Forensic DNA analysis is the application of modern DNA analysis technology to analyze the distribution and transmission rules of DNA genetic markers in the population, to determine the consistency and kinship of the analyzed samples, so as to identify the source samples of the samples, and to provide services for the investigation and judicial trial. Technical Support. Its analysis objects mainly involve various body fluids (spots), tissues, hair and epidermal exfoliated cells of the human body. At present, the technologies used for identification in forensic DNA analysis are mainly for short tandem repeats (Short tandem repeats, STR) and single nucleotide polymorphisms (Single nucleotide polymorphisms, SNP) typing. [000...
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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 倪铭莫少康严江伟伯晓晨
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA