A hypoxia responsive liposome preparation and its preparation method and application
A liposome preparation and liposome technology, applied in the field of biomedicine, can solve the problems of limited killing ability of cancer cells, damage to the structure of blood vessels, consumption of large oxygen, etc. Effect
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Embodiment 1
[0037] Embodiment 1, the preparation of liposome-AQ4N-chlorin e6 preparation
[0038] After weighing DPPC, DSPE-PEG, CH, and Ce6 according to the molar ratio of 6:0.5:4:0.5, they were dissolved in chloroform solution, and the chloroform solution was blown dry with nitrogen. Weigh AQ4N and dissolve it in phosphate buffer to prepare a 10 mg / mL AQ4N solution and add the prepared solution into liposome-chlorin e6, stir in a water bath at 50°C for 30 minutes, and place the prepared liposome - AQ4N- Chlorin e6 formulation extruded film.
Embodiment 2
[0039] Embodiment 2, the property detection of liposome-AQ4N-chlorin e6 preparation
[0040] The liposome-AQ4N-chlorin e6 preparation prepared in Example 1 was qualitatively detected, and transmission electron microscope detection, dynamic light scattering, and ultraviolet absorption were respectively carried out.
[0041] Among them, the results of transmission electron microscope detection are as follows: figure 1 As shown, the results show that the liposome-AQ4N-chlorin e6 preparation prepared in Example 1 is distributed in a monodisperse state in water, indicating that the liposome-AQ4N-chlorin e6 preparation prepared by the present invention Uniform particle size, well dispersed in water.
[0042] The results of dynamic light scattering were as follows: figure 2 As shown, the results show that the particle size of the liposome-AQ4N-chlorin e6 preparation prepared in Example 1 is mainly concentrated at about 100nm in water.
[0043] The results of the UV absorption tes...
Embodiment 3
[0044] Embodiment 3, the photodynamic activity detection of liposome-AQ4N-chlorin e6 preparation
[0045] Prepare the aqueous solution of liposome-AQ4N-chlorin e6 preparation, irradiate with 660nm laser, detect the singlet oxygen content, set up liposome-AQ4N-chlorin e6 aqueous solution group, liposome-chlorin e6 Phene e6 aqueous solution group, phosphate buffer saline group. see results Figure 4 .
[0046] Figure 4 The results showed that the solutions containing the photosensitive molecule chlorin e6 all produced singlet oxygen after laser irradiation, and could be used as photodynamic therapy agents.
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