A method for preparing and regenerating protoplasts of Welan gum synthetic bacteria

Abstract

The invention provides a method for preparing and regenerating sphingomonas sp protoplast, and belongs to the technical field of microbial engineering. According to the method, sphingomonas sp is used as an original strain; the sphingomonas sp protoplast is prepared by an enzymolysis method; the formation rate of the obtained protoplast is 97.3 percent; and the regeneration rate is 33.9 percent. The method has the advantages that the operation is simple and convenient; the preparation rate of the protoplast is high; the activity is high; and the further breeding of the excellent sphingomonas sp strain by using protoplast fusion and genome shuffling is facilitated.

Description

technical field

[0001] The invention belongs to the field of microbial engineering, and in particular relates to a method for preparing and regenerating protoplasts of Welan gum-synthesizing bacteria. Background technique

[0002] Welan gum is a kind of microbial metabolic glue, which is a polysaccharide product synthesized by Sphingomonas strains. It has excellent rheological properties, and its viscosity is relatively stable to temperature, pH and metal salt ions, especially It has good high temperature resistance, and the viscosity of Welan gum solution changes little in the range of 25-100°C. As a thickener, suspending agent, emulsifier, stabilizer and lubricant, Welan gum is widely used in food, concrete, petroleum, chemical and other fields. However, compared with the well-developed microbial metabolic gums such as xanthan gum, there are still some problems in the fermentation production of Welan gum, especially the low fermentation yield, less gum content in the ferm...

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