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Method for producing soybean isoflavone

A technology of soybean isoflavones and soybeans, applied in the agricultural field, can solve the problems of waste, loss of processing value, deterioration, etc.

Inactive Publication Date: 2017-05-10
重庆泰升生态农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the germ of a fresh seed is removed by this method, the removed cotyledon must be processed immediately, otherwise it will deteriorate quickly, lose the value of processing, and cause waste
Therefore, although the content of soybean isoflavones in the germ is very high, which is 8 to 10 times that in the cotyledon, the production of soybean isoflavones by this method is also very limited due to the limited availability and yield of the germ.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Choose high-quality soybean varieties, take healthy and mature soybean seeds, disinfect them with chlorine for 2-10 hours, and inoculate the soybean navel down to B 5 In the solid medium, the pH of the medium is 5.8. Soybean seeds inoculated on the medium are cultivated for 7 days at 24°C-26°C to obtain healthy sterile soybean seedlings.

[0020] 2. Take sterile soybean seedlings and infect the susceptible parts of the sterile seedlings with activated Agrobacterium rhizogenes. After hairy roots grow from the infected part, the hair roots are cut off, placed on MS solid medium, and cultured at 25°C for 15-20 days.

[0021] 3. Take out the hair roots, smash and grind, extract with 70% ethanol, extract with 0.5g sample, 5ml ethanol, dilute the extracted solution 100 times, and use UV spectrophotometer at 250 and 260nm respectively Measure the absorbance of the extract. Select the sample (hairy root) whose absorbance value is higher than that of the control root, record and...

Embodiment 2

[0023] 1. Select high-yield soybean variety Dongnong 42, select plump, disease-free soybean seeds of the year, soak the soybean seeds in the supersaturated solution of bleaching powder tablets for 15 minutes, then rinse with sterile water 3 times to inoculate the seeds to solid B 5 On minimal medium, B 5 The Ph value of the basic medium is 5.8, cultured at 25°C for 10 days to obtain sterile seedlings, and the activated Agrobacterium is used to inoculate the sterile seedling cotyledons or hypocotyls. The specific method is: lightly cut the cotyledon and hypocotyl epidermis with a scalpel, and smear the wound with Agrobacterium. Two weeks later, the starting roots are grown in the wound, the hairy roots are cut off, sterilized with antibiotics, or in B 5 Add antibiotics to the basic medium to achieve the purpose of sterilization. Transfer the completely sterilized hair root to B 5 Hairy root culture on solid medium. The soy isoflavone content in the hairy root is 4-10 times that ...

Embodiment 3

[0025] Select high-yield soybean variety Hefeng 35, select plump, disease-free soybean seeds of the year, sterilize with chlorine for 2 hours to 24 hours, and inoculate the sterilized seeds into a solid medium composed of MS inorganic components and B5 organic components Above, the Ph value of the medium is 5.8, cultured at 25°C for 7-10 days to obtain sterile seedlings. Cut the hypocotyl of the sterile seedling, take activated Agrobacterium and smear it on the cut surface of the hypocotyl section under the sterile seedling. After 1 week to 2 weeks, start roots in the wound. Cut the hairy roots and place them in a medium with antibiotics for antibacterial culture. After sterilization is complete, transfer to MS liquid medium to cultivate hairy roots. The soy isoflavone content in the hairy root is 4-10 times that of the control root.

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Abstract

The invention relates to a method for producing soybean isoflavone in the technical field of agriculture. The method comprises steps as follows: S1, obtaining of sterile soybean seedlings: manure soybean seeds are taken and disinfected with chlorine, a germination medium is inoculated with the soybean seeds with hila facing downwards, and the sterile soybean seedlings are obtained; S2, infection with agrobacterium tumefaciens: different parts of the sterile soybean seedlings obtained in S1 are infected with activated rooting agrobacterium tumefaciens, hairy roots are cut off after growing at the infected parts and placed in an expanded propagation medium for growth; S3, extraction of soybean isoflavone: soybean isoflavone is extracted from the hairy roots obtained in S2. The problem that the biomass of soybean isoflavone extracted from germs is small despite high content is solved, and the effect of efficiently producing soybean isoflavone can be achieved.

Description

Technical field [0001] The invention relates to a method in the field of agricultural technology, in particular to a method for producing soybean isoflavones. Background technique [0002] Soybean isoflavones (soybean isoflavones) are secondary metabolites formed during the growth of soybeans, one of the bioflavonoids, and also a kind of phytoestrogens. Soy isoflavones found naturally in soybeans are polyphenolic compounds, which are mainly distributed in the cotyledons and hypocotyls of soybean seeds. The content of the seed coat is very small. 80%-90% of isoflavones are present in the cotyledons. It is 0.1%-0.3%. The isoflavones contained in hypocotyls are more diverse and high in concentration, ranging from 1% to 2%, but because embryos only account for 2% of the total weight of seeds, although the concentration is high, the proportion is very small (10%- 20%). [0003] Soy isoflavones are a very important non-nutrient component in soybeans. They have anti-oxidation, anti-can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/48
CPCA61K36/48A61K2236/10A61K2236/333
Inventor 黄文君夏天雨
Owner 重庆泰升生态农业发展有限公司
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