Trichoderma harzianum solid-state fermentation method
A technology of Trichoderma harzianum and solid-state fermentation, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of small promotion range and high cost, and achieve air pollution reduction, low cost, and reasonable ratio Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
specific Embodiment approach 1
[0020] Specific embodiment one: a kind of Trichoderma harzianum solid-state fermentation method of present embodiment, carry out according to the following steps:
[0021] One, prepare PDA slant medium and inoculate Trichoderma harzianum, cultivate at room temperature for 4 to 6 days, then wash the conidia of Trichoderma harzianum on the PDA slant medium with sterilized water, and prepare a concentration of 10 7 ~10 8 spores / mL of spore suspension;
[0022] Two, take by weight percentage 73.5% straw, 25% wheat bran, 0.5% cornmeal, 0.5% soybean flour and 0.5% (NH 4 ) 2 SO 4 , add tap water until the humidity is 300%, stir well and put it into polypropylene bacteria bags, each bag is 150g, seal with a gas-permeable lid, and then sterilize at 121°C for 20min to obtain the sterilized fermentation material;
[0023] 3. Add 2 mL of the spore suspension in step 1 to the sterilized fermentation material, then place it in a culture room with natural light, and cultivate it for 15 d...
specific Embodiment approach 2
[0034] Specific embodiment two: what this embodiment is different from specific embodiment one is, the preparation of PDA slant medium in step one: 200g potato, 20g glucose, 15g agar and 1000mL water, pack in the large polypropylene test tube, every tube 20mL , After sterilizing at 115°C for 30 minutes, place the inclined plane. Other steps and parameters are the same as those in Embodiment 1.
specific Embodiment approach 3
[0035] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in step 1, culture at room temperature for 5 days. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com