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A kind of solid state fermentation method of Trichoderma harzianum

A technology of Trichoderma harzianum and solid-state fermentation, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of high cost and small promotion range, achieve low cost, reduce air pollution, and save resources Effect

Active Publication Date: 2019-08-06
NORTHEAST FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of high cost and small popularization range in the existing Trichoderma fermentation method, and the problem of straw reuse, and provide a solid-state fermentation method for Trichoderma harzianum

Method used

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  • A kind of solid state fermentation method of Trichoderma harzianum
  • A kind of solid state fermentation method of Trichoderma harzianum
  • A kind of solid state fermentation method of Trichoderma harzianum

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specific Embodiment approach 1

[0020] Specific embodiment one: a kind of Trichoderma harzianum solid-state fermentation method of present embodiment, carry out according to the following steps:

[0021] One, prepare PDA slant medium and inoculate Trichoderma harzianum, cultivate at room temperature for 4 to 6 days, then wash the conidia of Trichoderma harzianum on the PDA slant medium with sterilized water, and prepare a concentration of 10 7 ~10 8 spores / mL of spore suspension;

[0022] Two, take by weight percentage 73.5% straw, 25% wheat bran, 0.5% cornmeal, 0.5% soybean flour and 0.5% (NH 4 ) 2 SO 4 , add tap water until the humidity is 300%, stir well and put it into polypropylene bacteria bags, each bag is 150g, seal with a gas-permeable lid, and then sterilize at 121°C for 20min to obtain the sterilized fermentation material;

[0023] 3. Add 2 mL of the spore suspension in step 1 to the sterilized fermentation material, then place it in a culture room with natural light, and cultivate it for 15 d...

specific Embodiment approach 2

[0034] Specific embodiment two: what this embodiment is different from specific embodiment one is, the preparation of PDA slant medium in step one: 200g potato, 20g glucose, 15g agar and 1000mL water, pack in the large polypropylene test tube, every tube 20mL , After sterilizing at 115°C for 30 minutes, place the inclined plane. Other steps and parameters are the same as those in Embodiment 1.

specific Embodiment approach 3

[0035] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that in step 1, culture at room temperature for 5 days. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.

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Abstract

The invention provides a Trichoderma harzianum solid-state fermentation method, relating to a solid-state fermentation method. The method solves the problems of high cost and small popularization range in the existing Trichoderma fermentation method, and the problem of reutilization of straws. The method comprises the following steps: 1. preparing a spore suspension; 2. preparing a fermentation material, and sterilizing; 3. inoculating the bacterium into the fermentation material, and carrying out fermentation culture; and 4. drying the fermentation product, screening, and collecting the spore powder. The Trichoderma harzianum solid-state fermentation method well utilizes the straws, thereby saving the resources, reducing the air pollution caused by field burning, and effectively implementing resource recycling and reutilization. The most effective Trichoderma spore powder is produced at the minimal cost, thereby providing the raw materials for the subsequent preparation of Trichoderma biological bacterial fertilizers and biological pesticide preparations. The solid-state fermentation method does not need large-scale equipment, does not need to strictly control the production conditions, has the advantages of low requirements for the operating environment and simple and easy-to-learn process, and is suitable for large-scale popularization and convenient for small-scale production.

Description

technical field [0001] The invention relates to a solid-state fermentation method. Background technique [0002] Trichoderma (Trichoderma) belongs to the subphylum Deuteromycota, Hyphospora, Hyphospora, and the genus Trichoderma. With the three characteristics of disease resistance, growth promotion and soil improvement, countries all over the world have applied Trichoderma to agricultural and forestry production. The most widely used Trichoderma is Trichoderma harzianum. The inoculant of Trichoderma harzianum is mainly spore preparation, therefore, mass production of Trichoderma spore powder can ensure the supply of preparation raw materials. [0003] The small-scale fermentation of Trichoderma often leads to failure of fermentation due to poor condition control. However, the current fermentation method has problems such as high cost, high operating environment requirements, and complicated process, which are not suitable for large-scale promotion and use. [0004] Rice ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/885
CPCC12N1/14
Inventor 刘志华周畅王玉成王金杰范海娟
Owner NORTHEAST FORESTRY UNIVERSITY
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