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Rapid identification method of CMV and application of rapid identification method

An identification method and virus technology, applied in the field of agricultural detection, can solve problems such as high cost, wrong judgment, roughness, etc., and achieve the effects of low cost, convenient identification and reliable results

Inactive Publication Date: 2017-05-10
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, although the prevention and treatment of CMV virus is relatively mature, how to detect and identify CMV virus in time has become a key to the removal of CMV virus. Conventional CMV virus identification includes serological identification methods, double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and antigen direct coating method, electron microscope method, etc., the above-mentioned identification methods require high technical and equipment requirements, or high cost, etc., which are not convenient for planters to use, and the general experience judgment is relatively rough , often erroneous judgments

Method used

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  • Rapid identification method of CMV and application of rapid identification method
  • Rapid identification method of CMV and application of rapid identification method
  • Rapid identification method of CMV and application of rapid identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0014] Using the CMV rapid identification method of the present invention in tobacco planting

[0015] Tobacco is planted in the tobacco field, and several rows are planted at intervals in the tobacco ridge to identify hosts, including ordinary tobacco, heart leaf tobacco, cucumber or zucchini, amaranth, amaranth, and datura.

[0016] During the planting period, if mosaic leaves appear on the tobacco leaves, the tobacco leaves with more serious lesions are picked, then ground, and the ground juice is rubbed with the leaves to identify the host.

[0017] After friction, each of the following situations occurs:

[0018]

[0019] Then it can be judged that the infected virus is CMV virus.

[0020] Perform double antibody sandwich ELISA assay for CMV for verification

[0021] Utilize triple antibody sandwich ELISA to cucumber mosaic virus, the assay result shows that the antibody is positive, namely contains CMV virus.

[0022] After the CMV virus is detected, the following ...

Embodiment 2

[0026] Handle with embodiment 1, as after rubbing, each following situation occurs:

[0027]

[0028] Then it can be judged that the infected virus is not CMV virus, and it is verified as TMV virus by electron microscope.

[0029] The present invention starts from the traditional indication of plant lesions, and the creative expression is not only to select different indications of various hosts for different diseases to form a scientific and reasonable combination, but also to distinguish different diseases from each other, that is, the same Identifying the host system has different indication effects for different diseases and prevents duplication. It not only achieves the identification of diseases, but also distinguishes different diseases.

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Abstract

The invention provides a rapid identification method of a CMV. The method comprises the steps of transmitting the CMV in an identification host system and carrying out judgment according to representation of different hosts in the identification host system, wherein the identification hosts in the identification host system include nicotiana tabacum, nicotiana glutinosa, cucumber or pumpkin, gomphrena globosa, chenopodium amaranticolor and datura; and the identification hosts are represented as follows after CMV transmission: the nicotiana tabacum is floral leaves, the nicotiana glutinosa is floral leaves, the cucumber or pumpkin is floral leaves, the gomphrena globosa is floral leaves or necrosis, the henopodium amaranticolor is necrosis and the datura is floral leaves or inoculated leaf necrosis. The method is simple, low in cost, reliable in structure, and can be popularized and used. The invention further relates to an application of the rapid identification method of the CMV in agricultural planting.

Description

technical field [0001] The invention relates to the field of agricultural detection, in particular to a rapid identification method of CMV virus and its application. Background technique [0002] In agricultural planting, insect pests and diseases have always been the hazards of the planting industry, which are related to the quality and yield of agricultural products, and sometimes even cause the failure of agricultural products. Therefore, the prevention and control of insect pests and diseases has always been extremely important in agricultural planting. Pesticides are an effective way to solve this problem, but with the deepening of the concept of environmental protection and the popularization of healthy food, people's demand for pollution-free and pesticide-free agricultural products continues to increase, and there are frequent pesticide residues in agricultural products on the market Exceeding the standard report, the damage of pesticide residues to human health is v...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/04C12R1/94
CPCC12Q1/04
Inventor 陈泽鹏
Owner GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
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