Fenza No.1 dwarf banana tissue culture rapid propagation method

Abstract

The invention discloses a Fenza No.1 dwarf banana tissue culture rapid propagation method, and belongs to the field of plant tissue culture. The method comprises the following steps: selecting a plant having green and stout cauloid, the height of 3.2-4.5 m, big fruit clusters, fewer fruit finger conjoined fruits, normal fruit shape and good quality as a mother plant; spraying absorptive buds on the mother plant with a carbendazim and streptomycin mixed liquid, 1 week later, taking the absorptive buds as explants, and inoculating; controlling a differentiation culture temperature to be 27 DEG C-33 DEG C, carrying out differentiation culture for 12-20 days, subculturing, and controlling the total number of subculture generations to be 12-16; and carrying out rooting culture and hardening seedlings. The Fenza No.1 rooting seedlings bred by the technology has more, stout and white root systems, pale yellow green plantlet cauloids, dark green leaves and high provisonal planting survival rate, and grow quickly and neatly; each explant can reproduce about 5000-8000 plantlets within a year, after the plantlets are fix-planted, the seed nature is stable, fewer variant plants appear, and banana farmers have good reflection.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a tissue culture rapid propagation method of Fenza No. 1 Fenjiao. Background technique [0002] 'Fenza No. 1' Fenjiao is a newly selected and bred Fenjiao variety resistant to Fusarium wilt. It was researched and produced by the Fruit Tree Research Institute of Guangdong Academy of Agricultural Sciences and Zhongshan Agricultural Bureau. It is an occasional seedling of Guangfen 1 Fenjiao. [0003] At present, although the tissue culture and rapid propagation technology of bananas has been widely used, because the genotype of Fenza No. 1 Fenjiao is tetraploid banana ABBB type, the commonly used method of tissue culture and rapid propagation of triploid banana (AAA type) To cultivate Fenza No. 1, its propagation speed is slow, the efficiency is low, and the survival of rooted seedlings transplanted is low. Generally speaking, the multiplication multiple that can be o...

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Problems solved by technology

[0003] At present, although the tissue culture and rapid propagation technology of bananas has been widely used, because its genotype is the tetraploid banana ABBB type of Fenza No. To cultivate Fenza No. 1, its propagation speed is slow, the efficiency is low, and the survival of rooted seedlings transplanted is low. Generally speaking, the multiplication multiple that can be obtained is only 1.3 times. Survival rate sometimes less than 20%

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