Fenza No.1 dwarf banana tissue culture rapid propagation method

A tissue culture fast propagation and powdered banana technology, applied in the field of plant tissue culture, can solve the problems of low survival, low efficiency and slow reproduction speed of rooted seedling transplantation, achieve fast and neat growth, increase NAA concentration, and good effect. Effect

Active Publication Date: 2017-05-24
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, although the tissue culture and rapid propagation technology of bananas has been widely used, because its genotype is the tetraploid banana ABBB type of Fenza No. To cultivate Fenza No. 1, its propagatio

Method used

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  • Fenza No.1 dwarf banana tissue culture rapid propagation method
  • Fenza No.1 dwarf banana tissue culture rapid propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Implementation location: Female parent garden of Fruit Tree Research Institute of Guangdong Academy of Agricultural Sciences, time: October-November 2012.

[0047](1) Screening of mother plants: select new banana plants with green, strong pseudostems, a height of 3.2-3.5m, larger ears, less conjoined fruit, normal fruit shape, and good quality, and plant them in greenhouses or In the isolated plantation, it is used as the female parent garden for budding.

[0048] (2) Treatment of explant sucking buds: when the sucking buds grow to 30-50cm, spray the mixed liquid of 50% carbendazim 1000 times and 150ppm agricultural streptomycin on the sucking buds. Specifically, 1,000 kg of mixed medicinal solution contains 1 kg of 50% carbendazim, 150 grams of agricultural streptomycin, and 0.5 kg of mixed medicinal solution per bud; 1 week later, the suction buds are taken as explants for inoculation.

[0049] (3) Differentiation culture: Glass bottles or polypropylene film bags are...

Embodiment 2

[0057] Implementation location: The female parent garden of the Institute of Fruit Trees, Guangdong Academy of Agricultural Sciences, time: October 2013.

[0058] (1) Screening of the mother plant: Select the newly planted banana with a green, strong pseudostem and a height of 3.4m and the perennial banana with a height of 4.1m. The ears are larger, the fruit fingers are less conjoined, the fruit shape is normal, and the quality is good. , as the female parent tree for budding.

[0059] (2) Treatment of sucking buds of explants: when the sucking buds grow to 30-50cm, spray the mixed liquid of 50% carbendazim 1000 times solution plus 150ppm agricultural streptomycin on the sucking buds, and pour the mixed medicine into each bud 0.5 kg of liquid; 1 week later, the suction buds were taken as explants for inoculation.

[0060] (3) Differentiation culture: Polypropylene film bags are used as packaging containers for differentiation culture, and the temperature is controlled at 29°...

Embodiment 3

[0066] Implementation location: The mother garden of the Institute of Fruit Trees, Guangdong Academy of Agricultural Sciences, time: October 2014.

[0067] (1) Screening of mother plants: select perennial Banana plants with green, strong pseudostems, a height of 4.0m, larger ears, less conjoined fruit, and normal fruit shape, as the female parent tree, for bud picking .

[0068] (2) Treatment of sucking buds of explants: When the sucking buds grow to 40cm, spray the mixed liquid of 50% carbendazim 1000 times solution plus 150ppm agricultural streptomycin on the sucking buds, and pour 0.5 kg; 1 week later, the sucking buds were taken as explants for inoculation.

[0069] (3) Differentiation culture: Glass bottles are used as packaging containers for differentiation culture, and the temperature is controlled at 29°C to 33°C. Differentiate and cultivate for 17 days to subculture. When subculture transfers differentiated buds, cut off the upper part of the sheath leaf that cuts ...

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Abstract

The invention discloses a Fenza No.1 dwarf banana tissue culture rapid propagation method, and belongs to the field of plant tissue culture. The method comprises the following steps: selecting a plant having green and stout cauloid, the height of 3.2-4.5 m, big fruit clusters, fewer fruit finger conjoined fruits, normal fruit shape and good quality as a mother plant; spraying absorptive buds on the mother plant with a carbendazim and streptomycin mixed liquid, 1 week later, taking the absorptive buds as explants, and inoculating; controlling a differentiation culture temperature to be 27 DEG C-33 DEG C, carrying out differentiation culture for 12-20 days, subculturing, and controlling the total number of subculture generations to be 12-16; and carrying out rooting culture and hardening seedlings. The Fenza No.1 rooting seedlings bred by the technology has more, stout and white root systems, pale yellow green plantlet cauloids, dark green leaves and high provisonal planting survival rate, and grow quickly and neatly; each explant can reproduce about 5000-8000 plantlets within a year, after the plantlets are fix-planted, the seed nature is stable, fewer variant plants appear, and banana farmers have good reflection.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a tissue culture rapid propagation method of Fenza No. 1 Fenjiao. Background technique [0002] 'Fenza No. 1' Fenjiao is a newly selected and bred Fenjiao variety resistant to Fusarium wilt. It was researched and produced by the Fruit Tree Research Institute of Guangdong Academy of Agricultural Sciences and Zhongshan Agricultural Bureau. It is an occasional seedling of Guangfen 1 Fenjiao. [0003] At present, although the tissue culture and rapid propagation technology of bananas has been widely used, because the genotype of Fenza No. 1 Fenjiao is tetraploid banana ABBB type, the commonly used method of tissue culture and rapid propagation of triploid banana (AAA type) To cultivate Fenza No. 1, its propagation speed is slow, the efficiency is low, and the survival of rooted seedlings transplanted is low. Generally speaking, the multiplication multiple that can be o...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 黄秉智杨护许林兵吴元立杨兴玉肖维强
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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