Bactericide composition containing azoxystrobin, and preparation method and application thereof

A fungicide composition, azoxystrobin technology, applied in the directions of botanical equipment and methods, fungicides, applications, etc., can solve problems such as threats to the production of tobacco seedlings

Active Publication Date: 2017-05-24
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the case of intensive floating seedling cultivation, the germs spread quickly, seriously threatening the production of tobacco seedlings

Method used

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  • Bactericide composition containing azoxystrobin, and preparation method and application thereof
  • Bactericide composition containing azoxystrobin, and preparation method and application thereof
  • Bactericide composition containing azoxystrobin, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of azoxystrobin-containing fungicide composition. Include the following steps:

[0032] (1) the mass concentration is that 95% azoxystrobin former medicine is made into 1 * 10 with methanol 4 μg / ml mother solution, stored at 4°C;

[0033](2) Put Bacillus amyloliquefaciens X60 (Bacillus amyloliquefaciens X60) in NB culture medium (preparation method: 3g of beef extract, 10g of peptone, add deionized water to 1L, pH6.8-7.2, sterilize at 120°C for 20 minutes ), shake culture at 180rpm at 30°C for 36-48 hours, then centrifuge at 10000rpm for 10 minutes, take X60 wet cells and cell preservation solution (thalli preservation solution contains Tween 40 of 0.01mol / L, phosphate buffer 0.001mol / L non-ionic surfactant), formulated as bacterial agent according to the mass volume ratio of 1:50, the concentration of viable bacteria in the finished bacterial agent is 1×10 10 -1×10 12 CFU / ml;

[0034] (3) Azoxystrobin was mixed with the wet cells of Bacillus a...

Embodiment 2

[0036] A preparation method of azoxystrobin-containing fungicide composition. Include the following steps:

[0037] (1) the mass concentration is that 95% azoxystrobin former medicine is made into 1 * 10 with methanol 4 μg / ml mother solution, stored at 4°C;

[0038] (2) Put Bacillus amyloliquefaciens X60 (Bacillus amyloliquefaciens X60) in NB culture medium (preparation method: 3g of beef extract, 10g of peptone, add deionized water to 1L, pH6.8-7.2, sterilize at 120°C for 20 minutes ), shake culture at 180rpm at 30°C for 36-48 hours, then centrifuge at 10000rpm for 10 minutes, take X60 wet cells and cell preservation solution (thalli preservation solution contains 0.01mol / L phosphate buffer 0.001mol / L Tween 40 non- Ionic surface active agent), according to the mass volume ratio of 1:50 to prepare the bacterial agent, the concentration of viable bacteria in the finished bacterial agent is 1×10 10 -1×10 12 CFU / ml;

[0039] (3) Azoxystrobin was mixed with the wet cells of B...

Embodiment 3

[0041] A preparation method of azoxystrobin-containing fungicide composition. Include the following steps:

[0042] (1) the mass concentration is that 95% azoxystrobin former medicine is made into 1 * 10 with methanol 4 μg / ml mother solution, stored at 4°C;

[0043] (2) Put Bacillus amyloliquefaciens X60 (Bacillus amyloliquefaciens X60) in NB culture medium (preparation method: 3g of beef extract, 10g of peptone, add deionized water to 1L, pH6.8-7.2, sterilize at 120°C for 20 minutes ), shake culture at 180rpm at 30°C for 36-48 hours, then centrifuge at 10000rpm for 10 minutes, take X60 wet cells and cell preservation solution (thalli preservation solution contains 0.01mol / L phosphate buffer 0.001mol / L Tween 40 non- Ionic surface active agent), according to the mass volume ratio of 1:50 to prepare the bacterial agent, the concentration of viable bacteria in the finished bacterial agent is 1×10 10 -1×10 12 CFU / ml;

[0044] (3) Azoxystrobin was mixed with the wet cells of B...

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PUM

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Abstract

The invention discloses a bactericide composition containing azoxystrobin, and a preparation method and application thereof. The concentration of the azoxystrobin is 20 to 300 microgram / ml, the concentration of bacillus amyloliquefaciens X60 is 1*10<6> to 1*10<10> CFU / ml. The preparation method comprises the following steps: preparing a mother solution of 1*10<4> microgram / ml from an azoxystrobin active compound with a mass concentration of 95 percent by using methanol, and storing the mother solution at a low temperature of 4 DEG C; the concentration of living bacteria in the bactericide finished product is 1*10<10> to 1*10<12> CFU / ML; and mixing azoxystrobin and bacillus amyloliquefaciens X60 wet cells, and preparing the composition with the azoxystrobin concentration of 20 to 300 microgram / ml and the concentration range of the bacillus amyloliquefaciens X60 being 1*10<6> to 1*10<10> CFU / ml. The bactericide composition has good effect on preventing alternaria alternate, erysiphe cichoracearum and tobacco gray mold and is environment-friendly.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a method for preparing the azoxystrobin-containing fungicide composition, and the use of the azoxystrobin-containing fungicide composition in preventing and treating tobacco red spot disease and tobacco white powder. disease and tobacco gray mold. Background technique [0002] Tobacco brown spot and tobacco powdery mildew are fungal diseases caused by Alternaria alternateta (Fr.) Keissl and Erysiphecichoracearum DC respectively during the growing season of tobacco. They all have the characteristics of intermittent and explosive. In severe cases, the incidence rate of tobacco leaves can be as high as 90%, which reduces the quality of flue-cured tobacco by 1-2 grades, and reduces production and income by more than 50% in severely diseased areas. In recent years, tobacco red spot disease and tobacco powdery mildew have been one of the main diseases that seriously threaten the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N43/54A01P3/00
Inventor 汪汉成李文红兰树斌商胜华孟建玉贾蒙骜曹毅陈兴江陆宁
Owner GUIZHOU TOBACCO SCI RES INST
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