A method for enhancing the content of total vinegar total acid ester and reducing sugar by using high-yield indigenous aroma yeast to strengthen Daqu
An indigenous technology for producing aroma and sugar content, which can be applied to biological materials and their application fields, and can solve the problems of increasing production, declining vinegar quality, and large strengthening.
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[0017] A method for increasing the content of total vinegar total acid esters and reducing sugars by using high-yielding indigenous aroma yeast, comprising the steps of:
[0018] 1. Isolation of indigenous aromatic yeasts with high ester yield: collect fermented grains fermented on the third and sixth days of traditional Shanxi old mature vinegar technology, and use Bengal red selection medium to separate the yeast in them by 10-fold descending gradient dilution method, and take Each 100uL of the gradient dilution sample solution was spread on the plate of Bengal Red selection medium, cultured upside down at 28°C for 48 hours, and a single colony with obvious colony characteristics was selected and further purified by streaking and culturing for 2 to 3 times on the Bengal Red selection medium. Transfer to YEPD solid slant, store at 4°C, and use for later use; the results show that: in the sample on the third day, the plate is covered with mold hyphae, and a large number of yeas...
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