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A method for enhancing the content of total vinegar total acid ester and reducing sugar by using high-yield indigenous aroma yeast to strengthen Daqu

An indigenous technology for producing aroma and sugar content, which can be applied to biological materials and their application fields, and can solve the problems of increasing production, declining vinegar quality, and large strengthening.

Inactive Publication Date: 2020-05-22
SHANXI AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to solve the problem that it is difficult to increase the output and improve the quality of Daqu by strengthening Daqu with exogenous non-indigenous yeasts, but instead cause the quality of vinegar to decline, the present invention provides a method of using high-yielding indigenous aroma-producing yeasts to increase the total esters of vinegar total acid, Method for reducing sugar content

Method used

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  • A method for enhancing the content of total vinegar total acid ester and reducing sugar by using high-yield indigenous aroma yeast to strengthen Daqu
  • A method for enhancing the content of total vinegar total acid ester and reducing sugar by using high-yield indigenous aroma yeast to strengthen Daqu
  • A method for enhancing the content of total vinegar total acid ester and reducing sugar by using high-yield indigenous aroma yeast to strengthen Daqu

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Embodiment Construction

[0017] A method for increasing the content of total vinegar total acid esters and reducing sugars by using high-yielding indigenous aroma yeast, comprising the steps of:

[0018] 1. Isolation of indigenous aromatic yeasts with high ester yield: collect fermented grains fermented on the third and sixth days of traditional Shanxi old mature vinegar technology, and use Bengal red selection medium to separate the yeast in them by 10-fold descending gradient dilution method, and take Each 100uL of the gradient dilution sample solution was spread on the plate of Bengal Red selection medium, cultured upside down at 28°C for 48 hours, and a single colony with obvious colony characteristics was selected and further purified by streaking and culturing for 2 to 3 times on the Bengal Red selection medium. Transfer to YEPD solid slant, store at 4°C, and use for later use; the results show that: in the sample on the third day, the plate is covered with mold hyphae, and a large number of yeas...

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Abstract

The invention belongs to the technical fields of biological materials and application thereof. In order to solve the problems that the effects of increasing yield and improving quality cannot be easily achieved by using Daqu intensified by exogenous non-indigenous yeasts, and on the contrary, the quality of vinegar is declined, the invention provides a method for increasing the contents of total acid, total ester and reducing sugar in table vinegar by using Daqu intensified by high-ester-yield indigenous aroma-producing yeast. The method comprises the steps of separating the high ester yield indigenous aroma-producing yeast, carrying out single strain activation, intensifying the Daqu, and carrying out acetic fermentation. The strain is separated out from traditional Shanxi mature vinegar Daqu, and is unique; the ester-producing ability of the strain is remarkably higher than that of commercialized angel aroma-producing yeast, so that the strain has very excellent high temperature resistance, acid resistance, ethanol tolerance and osmotolerance; after the Daqu is intensified by the strain, the problem of incompatibility of strains in a brewing process is solved; no matter the Daqu is intensified by double strains or a single strain according to the method provided by the invention in the actual production, the contents of the total acid, the total ester and the reducing sugar in the vinegar are obviously increased, and other two indexes, i.e., non-volatile acid and amino state nitrogen of the vinegar are not influenced.

Description

technical field [0001] The invention belongs to the technical field of biological materials and their applications, and in particular relates to a method for increasing the content of total vinegar total acid esters and reducing sugars by using high-yielding indigenous aroma-producing yeast. Background technique [0002] Different regions in China have their own local types of vinegar. Shanxi Old Mature Vinegar is a representative product of Shanxi Province, with a production history of more than 3,000 years. Due to its excellent and unique flavor and nutritional and health functions, it is known as the first of the four famous vinegars in China. The brewing process of Shanxi Mature Vinegar is very complicated and has been listed as a national intangible cultural heritage. Its unique quality is also closely related to the local water, climate, soil, ecological system, etc., because these factors determine the quality of Daqu. In the brewing process of Shanxi mature vinegar,...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N1/14C12J1/04C12R1/84C12R1/72
CPCC12J1/04
Inventor 霍乃蕊王媛慧陈红梅李宝霞王如福侯红萍武朝霞张也朱芷葳唐中伟
Owner SHANXI AGRI UNIV
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