Cigarette capable of dispelling alcohol effect and preparing method thereof
A technology for cigarettes and sobering up, which is applied in the fields of tobacco, processing tobacco, and manufacturing cigars, etc., to achieve the effect of stabilizing the technical effect of sobering up
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Embodiment 1
[0082] Example 1 Animal Drunkenness Experimental Model Establishment
[0083] In order to make the results of animal intoxication and hangover experiments scientific and repeatable, this paper establishes an animal intoxication experimental model of the relationship between the dose of alcohol used in the experiment and the phenomenon of animal intoxication, which is used for the research and evaluation of anti-alcohol substances. The specific content of the established model is mainly to determine the appropriate intragastric dose for drunken mice and determine the lethal intragastric dose for mice.
[0084] 1 Experimental materials
[0085] SPF-grade Kunming mice, weighing 20-25 g, male, were purchased from Shanghai Slack Experimental Animal Co., Ltd. (purchased from the Experimental Animal Center of Second Military Medical University).
[0086] 2 Main reagents and instruments
[0087] 56 ° Red Star Erguotou (Beijing Red Star Co., Ltd.), mouse gavage device, 1 ml syringe, ...
Embodiment 2
[0105] The preparation of embodiment 2 hovenia dulcis, kudzu flower, kudzu root extract
[0106] 2.1 Raw materials of Chinese herbal medicine
[0107] Pueraria lobata, Pueraria lobata, and Hovenia dulcis were purchased from Zhongda Pharmacy of Shanghai Leiyun Shangbei District Pharmaceutical Co., Ltd., respectively.
[0108] 2.2 Main reagents and instruments
[0109] Absolute ethanol (Sinopharm Chemical Reagent Co., Ltd.), 75% ethanol, deionized water (MiliQ pure water), acetonitrile, methanol, 0.1% phosphoric acid, pulverizer, 50-mesh sieve, rotary evaporator, Soxhlet extractor, 65 ℃ oven.
[0110] Preparation of puerarin standard product: Accurately weigh 1.97 mg of puerarin standard product (purchased from Shanghai Yuanye Biotechnology Co., Ltd.), dry at 60°C for 8 hours, dilute to 2 mL with 30% ethanol, take 1 mL to a 10 mL volumetric flask, That is, a 0.0985mg / mL puerarin reference solution was obtained.
[0111] Preparation of irisin standard substance: Accurately we...
Embodiment 3
[0144] Embodiment 3 best synergistic ratio experiment
[0145] 1 test material
[0146] Kunming mice (KM mice), weighing 20-25 g, male, were purchased from Shanghai Slack Experimental Animal Co., Ltd. (purchased from the Experimental Animal Center of Second Military Medical University).
[0147] 56 degrees Red Star Erguotou (Beijing Red Star Co., Ltd.), deionized water, mouse gavage device, 1 ml syringe, EP tube, pipette gun, pipette tip.
[0148] Preparation of positive control substance corn peptide solution: take 0.9g corn peptide and dissolve in 3mL deionized water to obtain 0.3g / mL corn peptide solution;
[0149] Preparation of Positive Control Substance Neptune Golden Bottle Solution: Take 3 pieces of Neptune Golden Bottle (Shanghai Dekang Medicine Store), crush them, dissolve in 4mL deionized water, and obtain 0.75g / mL Neptune Golden Bottle solution.
[0150] 2 Animals and their management
[0151] After the mice were purchased, they were acclimatized in the animal roo...
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