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A kind of bionic nerve graft for peripheral nerve repair and its preparation method

A peripheral nerve and nerve transplantation technology, applied in the field of bionic nerve grafts and their preparation, can solve problems such as poor repair effect of nerve conduits, and achieve the effect of good cell affinity and mechanical properties

Active Publication Date: 2019-11-12
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a bionic nerve graft for peripheral nerve repair and a preparation method thereof, aiming at the defect that the repair effect of the existing nerve guide is not good. Gradual orientation of fibrin hydrogel bundles to promote directional growth of nerve fibers

Method used

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  • A kind of bionic nerve graft for peripheral nerve repair and its preparation method
  • A kind of bionic nerve graft for peripheral nerve repair and its preparation method
  • A kind of bionic nerve graft for peripheral nerve repair and its preparation method

Examples

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preparation example Construction

[0052] see figure 1 , is a flowchart of a method for preparing a bionic nerve graft for peripheral nerve repair according to a preferred embodiment of the present invention. like figure 1 As shown, this embodiment provides the above-mentioned preparation method of the bionic nerve graft for peripheral nerve repair, comprising the following steps:

[0053] Step S1, preparation of chitosan film catheter. Preferably, the chitosan film conduit is prepared by solvent evaporation, specifically comprising:

[0054] Step S1-1, dissolving chitosan in any one of dilute glacial acetic acid and hydrochloric acid to prepare an acid solution of chitosan, wherein the concentration of chitosan is 0.01-0.02g / ml (such as 0.01, 0.012, 0.015 , 0.018 or 0.02g / ml). Preferably, the concentration of glacial acetic acid used in this step is 0.015g / ml, and the concentration of hydrochloric acid is 0.01g / ml. The chitosan is preferably carboxymethyl chitosan.

[0055] Step S1-2, taking the chitosan...

Embodiment 1

[0078] 1. Chitosan is dissolved in 2% glacial acetic acid aqueous solution to prepare an acid solution of chitosan, wherein the concentration of chitosan is 0.015g / ml.

[0079] 2. Take the chitosan acid solution in a glass petri dish with a flat surface, ventilate and dry at normal temperature and pressure, and wait for the solvent to volatilize to obtain a semi-dry chitosan solution.

[0080] 3. Dry the semi-dry chitosan solution in an oven at 60°C for 48 hours.

[0081] 4. Soak the obtained chitosan film in pure water and fully wash it for use. The thickness of the chitosan film is 0.4 mm.

[0082] 5. Dissolve the fibrinogen in pure water, and mix it evenly with the PEO aqueous solution to obtain the electrospinning stock solution. The mass fraction of fibrinogen in the electrospinning stock solution is 4%, and the mass fraction of PEO is 1.2%. The electrospinning stock solution was then injected into a syringe for use.

[0083] 6. Add CaCl 2 The aqueous solution was mi...

Embodiment 2 to 23

[0088] Examples 2 to 23 were carried out in substantially the same manner as Example 1 except for the contents of Table 1 below.

[0089]

[0090]

[0091]

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Abstract

The invention relates to a bionic nerve graft used for peripheral nervous recovery and a preparation method and application thereof. The bionic nerve graft comprises a chitosan film conduit and a fibrous protein hydrogel beam positioned in the chitosan film conduit, wherein the fibrous protein hydrogel beam is prepared from fibrous protein through an electrospinning technique. The bionic nerve graft prepared by the preparation method is formed by the filling of the chitosan film conduit and the fibrous protein hydrogel beam of a multi-stage orientation structure, wherein a nerve regrowth space is effectively supported by a chitosan film, the orientational regeneration of a nerve axon is effectively guided by the fibrous protein hydrogel beam of multi-stage orientation, the cell affinity and the mechanical property of whole materials are good, and in addition, degrading speed and the repairing speed of peripheral nervous tissues are matched.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a bionic nerve graft for repairing peripheral nerves and a preparation method thereof. Background technique [0002] The repair of nerve injury is a series of complex pathological processes. The slow nerve regeneration process, the formation of scar tissue, the generation of inhibitory microenvironment, the degeneration of motor terminal tissue and function all restrict the repair of damaged nerve. Small-segment nerve defects can be restored by the nerve's self-repair ability. The repair and functional reconstruction of long-segment nerve defects is still a clinical problem in neurosurgery, which seriously affects the health status and quality of life of patients. [0003] Nerve stump anastomosis and nerve conduit bridging are often used in surgical operations for the regeneration of damaged nerves. The former is suitable for small nerve injuries, and the treatment ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/20A61L27/22A61L27/52A61L27/50D01D5/00
CPCA61L27/20A61L27/225A61L27/50A61L27/52A61L2430/32D01D5/003D01D5/0069D01D5/0076D01D5/0092C08L5/08
Inventor 王秀梅曹峥杜金蓉姚生莲
Owner TSINGHUA UNIV
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