Bionic fibrous protein hydrogel micelle for spinal cord injury repair and preparation method of bionic fibrous protein hydrogel micelle

A fibrin, spinal cord injury technology, applied in cellulose/protein conjugated man-made filaments, fiber processing, fiber chemical characteristics, etc., can solve the problems of poor repair effect of nerve bridging materials, and achieve the effect of promoting directional growth

Inactive Publication Date: 2017-05-31
TSINGHUA UNIV
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AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a biomimetic fibrin hydrogel bundle for spinal cord injury repair and its preparation method for the defect of poor repair effect of existing nerve bridging materials, which can be generated by electrospinning Hierarchically oriented biomimetic fibrin hydrogel bundles to promote directional growth of nerve fibers

Method used

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  • Bionic fibrous protein hydrogel micelle for spinal cord injury repair and preparation method of bionic fibrous protein hydrogel micelle
  • Bionic fibrous protein hydrogel micelle for spinal cord injury repair and preparation method of bionic fibrous protein hydrogel micelle
  • Bionic fibrous protein hydrogel micelle for spinal cord injury repair and preparation method of bionic fibrous protein hydrogel micelle

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preparation example Construction

[0033] see figure 1 , is a flowchart of a method for preparing biomimetic fibrin hydrogel bundles for spinal cord injury repair according to a preferred embodiment of the present invention. Such as figure 1 As shown, this embodiment provides the above-mentioned preparation method of the biomimetic fibrin hydrogel bundle for spinal cord injury repair, comprising the following steps:

[0034]Step S1, dissolving fibrinogen in pure water or physiological saline, and mixing evenly with polyethylene oxide (PEO) aqueous solution or polyethylene oxide (PEO) physiological saline solution to obtain an electrospinning stock solution. The mass fraction of fibrinogen in the electrospinning stock solution is 4% to 8% (such as 4%, 5%, 6%, 7% or 8%), and the mass fraction of polyethylene oxide (PEO) is 0.1% to 0.8%. (eg 0.1%, 0.2%, 0.5% or 0.8%). The electrospinning stock solution was then injected into a syringe for use. Preferably, in this step, the mass fraction of fibrinogen in the el...

Embodiment 1

[0050] 1. Dissolve fibrinogen in pure water and mix it evenly with PEO aqueous solution to obtain electrospinning stock solution. The mass fraction of fibrinogen in the electrospinning stock solution is 5.5%, and the mass fraction of PEO is 0.5%. The electrospinning stock solution was then injected into a syringe for use.

[0051] 2. Add CaCl 2 The aqueous solution was mixed with the thrombin solution to obtain a cross-linking solution, which was then kept at 37°C for use. CaCl in the crosslinking solution 2 The mass fraction of the thrombin is 1.5%, and the thrombin content is 30Units / ml. The cross-linking solution was then taken into a metal rotating receiving pan for use.

[0052] 3. The electrospinning stock solution injected into the syringe is electrospun under the action of the propulsion pump, the injection speed of fibrinogen is adjusted to 3.2ml / h, and the loading voltage is 4kV, so that continuous and stable fibrinogen spinning can be obtained. Use a metal rota...

Embodiment 2 to 21

[0055] Examples 2 to 21 were carried out in substantially the same manner as Example 1 except for the contents of Table 1 below.

[0056]

[0057]

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Abstract

The invention relates to a bionic fibrous protein hydrogel micelle for spinal cord injury repair as well as a preparation method and application of the bionic fibrous protein hydrogel micelle. The bionic fibrous protein hydrogel micelle is prepared from fibrous proteins by an electrostatic spinning technology. The bionic fibrous protein hydrogel micelle prepared by the single-channel electrostatic spinning technology is a hydrogel material with high water content and is formed by multi-stage oriented nano-fibers highly simulating spinal cord tissues; and the multi-stage oriented fibrous protein hydrogel micelle expresses compositions and extracellular matrix environment similar to natural spinal cord tissues, has the mechanical properties similar to natural nerves, can effectively promotes orientated growth of nerve fibers and has the degradation velocity matched with the repair velocity of the spinal cord injured tissues.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a bionic fibrin hydrogel bundle for repairing spinal cord injuries and a preparation method thereof. Background technique [0002] Spinal cord injury is one of the most common serious diseases in clinical practice. It has presented a situation of "three highs and one low" of high incidence, high disability rate, high cost and younger age in the world, and has become a major medical problem to be solved in the medical field. Spinal cord injury will lead to irreversible damage to the sensorimotor function of the central nervous system of the patient, seriously affecting the quality of life of the patient, and there is currently no effective treatment method and method for it in the world. [0003] The repair of nerve injury is a series of complex pathological processes. The slow nerve regeneration process, the formation of scar tissue, the generation of inhibitory micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/26A61L27/50D01F4/00D01F8/02D01F8/18D01D5/00
CPCA61L27/52A61L27/26A61L27/50A61L2430/32D01D5/003D01D5/0069D01D5/0076D01D5/0092D01F4/00D01F8/02D01F8/18C08L89/00C08L71/02C08L5/08C08L5/04
Inventor 王秀梅曹峥姚生莲
Owner TSINGHUA UNIV
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