Tissue critical plane model constructing method and three-dimensional culture cell micro-fluidic chip

A microfluidic chip and three-dimensional culture technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc. Incompatibility of the environment and other problems, to achieve the effect of convenient operation and three-dimensional growth

Pending Publication Date: 2017-05-31
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the modeling cycle of animal models is long, and it cannot realize real-time dynamic monitoring of the disease process, that is, it cannot realize the "panoramic" reproduction of the disease process; and the conventional two-dimensional cell culture, the microenvironment is too single, which is not related to the growth mode of cells in the body and its conditions. The microenvironment does not match, resulting in "distortion" of some results obtained

Method used

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  • Tissue critical plane model constructing method and three-dimensional culture cell micro-fluidic chip
  • Tissue critical plane model constructing method and three-dimensional culture cell micro-fluidic chip
  • Tissue critical plane model constructing method and three-dimensional culture cell micro-fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 A microfluidic chip for three-dimensional cultured cells

[0045] A microfluidic chip for three-dimensional cultured cells, comprising a chip layer 1 and a cover layer 2, wherein the chip layer 1 and the cover layer 2 are combined to contain 3 channels, the middle of the channel includes a connecting part 33, and the depth of the middle channel 31 is relatively deep. The other channels are shallow, and the shape of the channel is square, and the two ends of the channel are respectively provided with inlet and outlet holes 21 . The channels 32 on both sides are dispersedly arranged, the middle channel 31 is arranged in a straight line, the channels 32 on both sides are arranged symmetrically with the middle channel 31 as the axis, and the connecting parts 33 in the middle of the channels are evenly arranged. This arrangement can effectively separate the inlet and outlet holes 21 of each channel, and the operation is convenient. The three channels are horizonta...

Embodiment 2

[0046] Example 2 A microfluidic chip for three-dimensional cultured cells

[0047] A microfluidic chip for three-dimensional cultured cells, comprising a chip layer 1 and a cover layer 2, wherein the chip layer 1 and the cover layer 2 are combined to contain 3 channels, the middle of the channel includes a connecting part 33, and the depth of the middle channel 31 is relatively deep. The other channels are shallow, and the shape of the channel is square, and the two ends of the channel are respectively provided with inlet and outlet holes 21 . Also set the blocking cap 4 that covers the access hole 21, the size of the lower part of the blocking cap 4 is the same as the aperture of the access hole 21, and the size of the upper end is larger. blockage. The channels 32 on both sides are dispersedly arranged, the middle channel 31 is arranged in a straight line, the channels 32 on both sides are arranged symmetrically with the middle channel 31 as the axis, and the connecting par...

Embodiment 3

[0048] Example 3 A microfluidic chip for three-dimensional cultured cells

[0049] A microfluidic chip for three-dimensional cultured cells, including a chip layer 1 and a cover layer 2, wherein the chip layer 1 and the cover layer 2 are combined to contain 3 channels, the middle of the channel includes a connecting part 33, and the diameter of the middle channel 31 is larger than that on both sides The channel 32 is small; the channel shape is a circular or elliptical channel. The circular and elliptical settings are closer to the real lumen wall or blood vessel wall. Circular or elliptical channels are evenly arranged on the chip layer 1 and the cover layer 2 and assembled together, and each channel is provided with a connecting part 33 in the middle. Access holes 21 are respectively provided at both ends of the channel. Also set the blocking cap 4 covering the access hole 21, the size of the lower part of the blocking cap 4 is the same as the aperture of the access hole 2...

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Abstract

The invention relates to a combined culture cell micro-fluidic chip which comprises a chip layer and a covering layer, wherein the chip layer and the covering layer are combined and comprise a plurality of channels, the middles of the channels comprise communication portions, the depth of the middle channel is shallower than those of other channels, or the opening diameter of the middle channel is smaller than those of other channels, and an inlet / outlet is formed in each channel. The optimal number of the channels is three, the channels on the two sides are symmetrically scattered with the middle channel as the axis, the bottoms of all the channels are horizontal, and the inlet / outlets are formed in the two ends of each channel. The micro-fluidic chip can achieve three-dimensional growth of cells, can construct a tissue critical plane model in the middle, achieves an effect of observing disease mechanisms by injecting medicine, is reasonable in structure and convenient to use, can effectively establish a biological model and can observe the model situation in real time.

Description

technical field [0001] The invention belongs to a chip for building a model after cell culture, in particular to a microfluidic chip and a model building method for building a contact surface model for two or more cells, and specifically relates to a method for building a tissue critical surface model And a microfluidic chip for three-dimensional cultured cells. Background technique [0002] At present, clinical studies on disease mechanisms mostly rely on traditional animal modeling and two-dimensional cell culture methods to observe pathological phenomena. However, the modeling cycle of animal models is long, and it cannot realize real-time dynamic monitoring of the disease process, that is, it cannot realize the "panoramic" reproduction of the disease process; while the conventional two-dimensional cell culture, the microenvironment is too single, which is not related to the growth mode of cells in the body and the resulting conditions. The local microenvironment does no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00
CPCC12M23/16
Inventor 王辰代华平何佳芮
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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