Tissue critical plane model constructing method and three-dimensional culture cell micro-fluidic chip
A microfluidic chip and three-dimensional culture technology, applied in tissue cell/virus culture devices, biochemical equipment and methods, biochemical instruments, etc. Incompatibility of the environment and other problems, to achieve the effect of convenient operation and three-dimensional growth
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Embodiment 1
[0044] Example 1 A microfluidic chip for three-dimensional cultured cells
[0045] A microfluidic chip for three-dimensional cultured cells, comprising a chip layer 1 and a cover layer 2, wherein the chip layer 1 and the cover layer 2 are combined to contain 3 channels, the middle of the channel includes a connecting part 33, and the depth of the middle channel 31 is relatively deep. The other channels are shallow, and the shape of the channel is square, and the two ends of the channel are respectively provided with inlet and outlet holes 21 . The channels 32 on both sides are dispersedly arranged, the middle channel 31 is arranged in a straight line, the channels 32 on both sides are arranged symmetrically with the middle channel 31 as the axis, and the connecting parts 33 in the middle of the channels are evenly arranged. This arrangement can effectively separate the inlet and outlet holes 21 of each channel, and the operation is convenient. The three channels are horizonta...
Embodiment 2
[0046] Example 2 A microfluidic chip for three-dimensional cultured cells
[0047] A microfluidic chip for three-dimensional cultured cells, comprising a chip layer 1 and a cover layer 2, wherein the chip layer 1 and the cover layer 2 are combined to contain 3 channels, the middle of the channel includes a connecting part 33, and the depth of the middle channel 31 is relatively deep. The other channels are shallow, and the shape of the channel is square, and the two ends of the channel are respectively provided with inlet and outlet holes 21 . Also set the blocking cap 4 that covers the access hole 21, the size of the lower part of the blocking cap 4 is the same as the aperture of the access hole 21, and the size of the upper end is larger. blockage. The channels 32 on both sides are dispersedly arranged, the middle channel 31 is arranged in a straight line, the channels 32 on both sides are arranged symmetrically with the middle channel 31 as the axis, and the connecting par...
Embodiment 3
[0048] Example 3 A microfluidic chip for three-dimensional cultured cells
[0049] A microfluidic chip for three-dimensional cultured cells, including a chip layer 1 and a cover layer 2, wherein the chip layer 1 and the cover layer 2 are combined to contain 3 channels, the middle of the channel includes a connecting part 33, and the diameter of the middle channel 31 is larger than that on both sides The channel 32 is small; the channel shape is a circular or elliptical channel. The circular and elliptical settings are closer to the real lumen wall or blood vessel wall. Circular or elliptical channels are evenly arranged on the chip layer 1 and the cover layer 2 and assembled together, and each channel is provided with a connecting part 33 in the middle. Access holes 21 are respectively provided at both ends of the channel. Also set the blocking cap 4 covering the access hole 21, the size of the lower part of the blocking cap 4 is the same as the aperture of the access hole 2...
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