A microbial species identification device and a microbial species identification method

An identification method and microbial technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of single analysis object and complex pretreatment, and achieve the effect of safe and economical identification, microbial biomass feature fidelity and enrichment

Active Publication Date: 2019-02-12
CHINA UNIV OF PETROLEUM (BEIJING)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as mentioned above, the chemical classification method requires complex pretreatment of samples, and the analysis object is single.

Method used

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  • A microbial species identification device and a microbial species identification method
  • A microbial species identification device and a microbial species identification method
  • A microbial species identification device and a microbial species identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The present embodiment provides a thermal split gas chromatography device, such as figure 1 As shown, the thermal split gas chromatography device includes a solid-state sampling needle 1, a thermal split unit and a chromatographic analysis unit.

[0059] The thermal splitting unit includes a vertical tube furnace thermal splitter 2, a thermal splitting liner 8 and a quartz tube 7; the vertical tube furnace thermal splitter 2 includes a thermal splitting chamber 9 and a tubular electric furnace 11; the thermal splitting liner 8 runs through The interior of the thermal splitting chamber 9 is connected with the upper and lower inlets and outlets of the thermal splitting chamber 9, the tubular electric furnace 11 is set on the outer wall of the thermal splitting chamber 9, and the quartz tube 7 is a columnar tube body structure with a cavity.

[0060] During use, the front end of the solid-state sampling needle 1 is curved, and the inner wall of the lumen of the quartz tube...

Embodiment 2

[0065] The present embodiment provides a kind of microbial species identification method, and it adopts the thermal splitting gas chromatography device described in embodiment 1 to carry out, comprises the following steps:

[0066] Step 1, using the Escherichia coli (Escherichia coli) strain of the China General Microorganism Culture Collection and Management Center, numbered: 1.1100.10.4, enriching, culturing and centrifuging it, and then freeze-drying it to ensure its physical properties Constantly, obtain the microbial sample to be tested, then send in the sampling tube of thermal split gas chromatograph device;

[0067] Step 2, send the quartz tube to the inner cavity of the thermal splitting liner through the solid-state sampling needle, and quickly tighten it, turn on the tubular electric furnace to heat for thermal splitting treatment, and the temperature of the thermal splitting treatment is 400°C;

[0068] Step 3, after the thermal splitting treatment is finished, fee...

Embodiment 3

[0073] The present embodiment provides a kind of microbial species identification method, and it adopts the thermal splitting gas chromatography device described in embodiment 1 to carry out, comprises the following steps:

[0074] Step 1, using the fungus Pycnoporus coccineus isolated and purified by laboratory culture, enriching, culturing and centrifuging it, and then freeze-drying it to ensure that its physical properties remain unchanged to obtain a microbial sample to be tested, and then Into the sampling tube of thermal splitting gas chromatography device;

[0075] Step 2, send the quartz tube to the inner cavity of the thermal splitting liner through the solid-state sampling needle, and quickly tighten it, turn on the tubular electric furnace to heat for thermal splitting treatment, and the temperature of the thermal splitting treatment is 400°C;

[0076] Step 3, after the thermal splitting treatment is finished, feed the inert gas through the carrier gas inlet to swee...

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Abstract

The invention provides a microorganism species identification device and a microorganism species identification method. The microorganism species identification device comprises a sample feeding needle, a thermal splitting unit and a chromatographic analysis unit, wherein the thermal splitting unit comprises a vertical type tubular furnace thermal splitter, a thermal splitting liner and a sample feeding tube; the vertical type tubular furnace thermal splitter comprises a thermal splitting chamber and a heater; the thermal splitting liner penetrates through the thermal splitting chamber; the heater is arranged on the outer wall of the thermal splitting chamber in a sleeving manner; the front end of the sample feeding needle is fixedly connected with the inner wall of the cavity of the sample feeding tube; a gas carrying inlet is formed in the upper inlet of the thermal splitting chamber; the thermal splitting liner is communicated with the chromatographic analysis unit. When microorganism species are identified by using the microorganism species identification device, complex pretreatment process is not needed, chemical components which are liable to volatile or pyrolyze at different temperatures can be comprehensively analyzed and collected, obtained microorganisms are relatively real and rich in biomass characteristic, rapid, effective, safe and economic identification on the microorganisms can be achieved, and the microorganism species identification device and the microorganism species identification method can be applied to identification on various microorganisms.

Description

technical field [0001] The invention belongs to the technical field of microbial species identification, and relates to a microbial species identification device and a microbial species identification method. Background technique [0002] At present, the identification methods of microbial species mainly include phenotypic characteristics, chemical composition and DNA molecular biology methods. [0003] Compared with other microbial identification methods, this scheme has great advantages in terms of method and reliability. Phenotypic characteristics include morphological characteristics, physiological and biochemical characteristics, antibiotic sensitivity, phage typing, serological analysis, etc. With the advancement of technology, G+C mol%, DNA recombination test, DNA-DNA hybridization, PCR technology, 16S rDNA sequence sequencing and other molecular biology classification methods have appeared to make up for the defects in the classification of phenotypic characteristic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 万云洋朱迎佳江亮
Owner CHINA UNIV OF PETROLEUM (BEIJING)
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