A method for vaccination validity of a swine fever gene engineering sub -unit

A subunit vaccine and genetic engineering technology, which is applied in the field of swine fever genetic engineering subunit vaccine efficacy inspection, can solve the problems of long inspection period, and achieve the effects of short inspection period, saving inspection cost and high biosafety.

Active Publication Date: 2018-11-20
YEBIO BIOENG OF QINGDAO
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The genetically engineered subunit vaccine of CSFV is an inactivated vaccine. Due to the use of target animals (pigs) for immune challenge, there is a long detection cycle (about 51 days), and a strong virus (Shimen strain of CSFV) challenge test is required, which is urgently needed Establish an alternative animal or other efficacy testing method for the efficacy testing of inactivated vaccines to solve problems such as shortening the testing cycle, saving testing costs, and improving biosafety

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for vaccination validity of a swine fever gene engineering sub -unit
  • A method for vaccination validity of a swine fever gene engineering sub -unit
  • A method for vaccination validity of a swine fever gene engineering sub -unit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A kind of swine fever genetic engineering subunit vaccine potency test method, comprises the following steps:

[0021] (1) Take healthy susceptible (pestivirus ELISA antibodies are all negative) rabbits (body weight 1.5-3.0kg) as test objects, according to body weight, sex, 20 rabbits are randomly divided into 4 groups, each group 5 3 groups were used as immunization groups, which were respectively immunized with 201401, 201402, and 201403 batches of CSFV genetically engineered subunit vaccines (293T-E2), and 1 group was used as a control group;

[0022] (2) Subcutaneous intravenous injection is carried out to the neck of the rabbits in the immunized group, and the injection of the genetically engineered subunit vaccine (293T-E2) of classical swine fever virus (293T-E2) (0.2ml / only), while the rabbits of the control group do not inject any substance; The rabbits in the control group and the rabbits in the control group were raised according to the normal feeding method;...

Embodiment 2

[0031] The swine fever genetic engineering subunit vaccine (293T-E2) effectiveness test method described in embodiment 1, when carrying out the effectiveness test of immune challenge virus with pig:

[0032] Take 20 pigs aged 35-60 days who are healthy and susceptible (pestivirus PCR test is negative, ELISA antibody is negative), and randomly divide them into 4 groups, 5 pigs in each group; 1-3 groups are immune groups, and each head and neck is injected intramuscularly 201401, 201402, 201403 batches of CSFV genetic engineering subunit vaccine (293T-E2) 1.0ml / head; the fourth group is the control group. 35 days after immunization, all pigs were injected with 1.0ml / head of Shimen strain of classical swine fever virus; 2 days before the challenge, the temperature of the pigs was measured twice a day, and twice a day after the challenge, for a total of 16 days, and the challenge was observed. Clinical manifestations of pigs (conjunctivitis, loss of appetite or loss of appetite, a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for testing potency of a CFS (classical swine fever) genetic engineering subunit vaccine. After the CFS genetic engineering subunit vaccine (293T-E2) is injected into rabbits with negative CFS virus ELSIA (enzyme linked immunosorbent assay) antibody, immunization is performed for a period of time, then a CFS live vaccine (spleen and lymp tissue origin) is injected, and protection rate reaches 100% or higher. Results of rabbit based immunity challenge and swine based immunity challenge are consistent, so that the method can replace a testing method adopting swine for immunity challenge, the detection period is shortened, the detection cost is reduced, the operability is strong, the result is easy to determine, and biological safety is high.

Description

technical field [0001] The invention belongs to the technical field of vaccine detection, and in particular relates to a method for testing the efficacy of a swine fever genetic engineering subunit vaccine. Background technique [0002] Swine fever (slassical swine fever, CFS) is characterized by hemorrhage and fever, and can be acute or chronic. The currently used attenuated vaccines are mainly Chinese swine fever rabbitized attenuated vaccine, Japanese GPE vaccine and French Thireval vaccine. In the late 1970s, the epidemic form of swine fever changed a lot. Regional sporadic epidemics and the occurrence of atypical swine fever symptoms, especially in recent years when monoclonal antibodies have been used at home and abroad to detect C-strains, different reaction patterns have been found, and swine fever immunization has repeatedly failed, etc., indicating the antigenicity of swine fever virus There may be mutations, so some people have questioned the traditional attenua...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569
CPCG01N33/56983G01N2333/08
Inventor 刘蕾申洪银郭玉广邹桂荣李金积胡潇曹志
Owner YEBIO BIOENG OF QINGDAO
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap