Flow cytometry collecting lens and optical system of two-color laser flow cytometry

Abstract

The invention discloses a flow cytometry collecting lens and an optical system of a two-color laser flow cytometry. The collecting lens comprises a first flat convex lens, a positive meniscus lens, a first balsaming lens group and a second balsaming lens group in sequential arrangement, wherein the first balsaming lens group comprises a first biconvex lens and a first negative meniscus lens; the second balsaming lens group comprises a second negative meniscus lens and a second biconvex lens; the optical system of the two-color laser flow cytometry comprises a fluorescence collection lens group, a D-shaped reflecting mirror, a first fluorescence collection optical path and a second fluorescence collection optical path; the D-shaped reflecting mirror is arranged on a focal surface of the fluorescence collection lens group and form a 45-degree included angle with the focal surface of the fluorescence collection lens group; a plurality of dichroic filters arranged according to the equal-optical-length phase compensation method are arranged in the first fluorescence collection optical path and the second fluorescence collection optical path. The performance of the collecting lens is excellent; the light wave energy loss of each light path is small; in addition, the losses are consistent; great influence cannot be caused on the subsequent signal processing.

Description

technical field [0001] The invention belongs to the technical field of optical instruments, and relates to biological detection and cell analysis technologies, in particular to a collection lens of a flow cytometer and an optical system of a two-color laser flow cytometer. Background technique [0002] Flow cytometry and flow cytometry are a detection method for quantitative analysis and sorting of single cells or other biological particles. Several parameters were measured. Flow cytometry is called the CT of the laboratory. The liquid flow system of the flow cytometer focuses the cell-like liquid into a single-cell flow laminar flow, and the cells line up and pass through the laser irradiation area in sequence. The sample is irradiated with laser light, the scattered light of the cells, and the fluorescence excited from the dyes carried by the cells are emitted around the cells centered on the cells. Thus, cells can be seen as point sources of light. Through the collecti...

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Problems solved by technology

Therefore, a larger size receiver is required, or the position of the receiver needs to be readjusted to prevent the light from falling into the dead zone or outside the photosensitive zone of the receiver, resulting in invalid reception

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