Liquid chromatography pre-column derivatization method for detecting glyphosate in water

A liquid chromatography column and glyphosate technology, applied in the field of liquid chromatography pre-column derivatization method for detecting glyphosate in water, can solve the problems of complicated procedures, water pollution, low efficiency and the like, and achieve cost saving and work efficiency. Effect

Active Publication Date: 2017-06-13
厦门鉴科检测技术有限公司
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Problems solved by technology

[0002] Glyphosate (chemical formula: C 3 h 8 NO 5 P) is an effective active chemical component in many herbicides widely used. At present, the wastewater used to generate glyphosate is an acidic wastewater containing high concentrations of inorganic salts and high concentrations of organic matter, wherein the pollutants mainly include methanol and formaldehyde. , glyphosate, the COD mass concentration is about 48000-52000mg/L, and also contains about 15% of inorganic salts, such as sodium chloride (NaCl). Glyphosate dry powder and additives are sold as glyphosate aqueous solution with a mass fraction of 10%, but in this way, a large amount of inorganic s...

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  • Liquid chromatography pre-column derivatization method for detecting glyphosate in water
  • Liquid chromatography pre-column derivatization method for detecting glyphosate in water
  • Liquid chromatography pre-column derivatization method for detecting glyphosate in water

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Embodiment Construction

[0042] Such as figure 1 As shown, a liquid chromatography pre-column derivatization method for detecting glyphosate in water disclosed in this embodiment comprises the following steps:

[0043] S1. Make a standard curve

[0044] Use a 1mL pipette to pipette 0.0mL, 0.1mL, 0.2mL, 0.4mL, 0.8mL, 1.0mL of 1.00μg / mL glyphosate standard solution into six 10mL volumetric flasks, and dilute to each volume with pure water After constant volume, the concentration of glyphosate in each volumetric flask is 0.0ng / mL, 10ng / mL, 20ng / mL, 40ng / mL, 80ng / mL, 100ng / mL, and take 0.8mL of the standard solution of each concentration , add 0.2mL 5% (volume fraction) borate buffer solution and 0.2mL 1.0g / L 9-fluorenylmethyl trichloromethane acetone solution, mix well, and derivatize overnight at room temperature to obtain the standard curve of glyphosate derivative products , the pH of the borate buffer solution is 9.0, and the glyphosate standard solution is prepared from a glyphosate standard produ...

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Abstract

The invention discloses a liquid chromatography pre-column derivatization method for detecting glyphosate in water. The method comprises the steps as follows: S1, production of a standard curve; S2, preparation of a standard working solution; S3, preparation of a sample; S4, glyphosate derivatization; S5, online testing; S6, result determination; S7, quantitative measurement; S8, result calculation. With the adoption of the method, the glyphosate in water can be effectively detected and measured in the absence of a post-column derivatization instrument, a special detection chromatographic column and a post-column derivatization reagent, so that the laboratory cost is greatly saved, and the working efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of chromatographic detection and analysis, in particular to a liquid chromatography pre-column derivatization method for detecting glyphosate in water. Background technique [0002] Glyphosate (chemical formula: C 3 h 8 NO 5 P) is an effective active chemical component in many herbicides widely used. At present, the wastewater used to generate glyphosate is an acidic wastewater containing high concentrations of inorganic salts and high concentrations of organic matter, wherein the pollutants mainly include methanol and formaldehyde. , glyphosate, the COD mass concentration is about 48000-52000mg / L, and also contains about 15% of inorganic salts, such as sodium chloride (NaCl). Glyphosate dry powder and additives are sold as glyphosate aqueous solution with a mass fraction of 10%, but in this way, a large amount of inorganic salts and organic matter in the wastewater are brought to the farmland, which will...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
Inventor 熊刚游青陈高水陈儒梅李平林志杰
Owner 厦门鉴科检测技术有限公司
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