Kit for detecting neutrophile granulocyte respiratory burst function

Abstract

The invention discloses a kit for detecting a neutrophile granulocyte respiratory burst function. The kit comprises the following components: a PMA solution, a DHR123 solution, a catalase solution, an equilibrium liquid, washing liquid, a red blood cell lysis buffer and a diluent. The kit provided by the invention can be used for detecting the ability of neutrophile granulocyte on producing active oxygen, and is particularly applicable to clinically screening patients with chronic granulomatous diseases; can also be used for detecting a neutrophile granulocyte respiratory burst function change under other various physiological and pathological conditions, and evaluating the influence of a medicine on the neutrophile granulocyte respiratory burst function. The kit not only can be used for detecting a human sample, but also can be used for other species such as mice, rats, rabbits, dogs, cows and pigs. The kit is mainly based on a DHR123 flow cytometer analytic method, requires less blood volume, and has the advantages of simple operation, fast detection speed, low cost, high popularization performance, good repeatability, accurate and clear detection result, high specificity and easiness for analysis.

Description

technical field [0001] The invention relates to a kit for detecting the respiratory burst function of neutrophils, which belongs to the technical field of testing. Background technique [0002] After being stimulated by external pathogenic microorganisms, the normal body resists infection and performs immune defense functions through a series of innate and adaptive immune responses. Among them, innate immunity is the first line of defense acquired by the body innately. It appears early and responds quickly, and plays an important role in the process of eliminating pathogens by the body. Neutrophils are an important part of the innate immune system. During the process of phagocytosis of pathogens, neutrophils produce superoxide anion and other reactive oxygen complexes, that is, "respiratory burst", and participate in the body's non-specific anti-infection process. Defects of neutrophil respiratory burst function are common in patients with clinical chronic granuloma, as we...

AI Technical Summary

Problems solved by technology

However, at present, there are only a few specialized children's hospitals in Beijing and Shanghai that can conduct neutrophil respiratory burst function testing experiments, and most hospitals do not have the testing technology and conditions

Most suspected cases need to travel from other places to Beijing and Shanghai for relevant testing, which is costly and difficult to operate

In addition, since the testing methods of each hospital are not exactly the same, there is no uniform judgment standard

Especially for patients with autosomal recessive inheritance, since the respiratory burst function of neutrophils is not completely lost, experimental deviations can easily affect the final diagnostic results of clinicians

In addition, there is only one company selling related kits in the domestic market, but the influence of endogenous catalase on the experimental results has not been ruled out, which will lead to instability of experimental results

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