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Application of compound Harziphilone

A compound and agricultural fungicide technology, applied in the application field of the compound Harziphilone, can solve the problems of pest resistance and ecological environment pollution, affecting the yield and quality of winter jujube, and fruit pesticide residues, etc., achieving low product cost, easy degradation, and production technology. simple effect

Inactive Publication Date: 2017-06-27
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the adjustment of the agricultural industrial structure and the change of climatic conditions, Lygus aeruginosa has become the main pest that harms the winter jujube. Its adults and nymphs can eat the young leaves, flower buds and tender fruits of the winter jujube, seriously affecting the The output and quality of winter jujube, causing serious economic losses
[0003] For a long time, chemical control has been the main method for the prevention and control of Lygus japonica in production, which can easily cause a series of problems such as pesticide residues in fruits, insect resistance and ecological environment pollution. Insecticides of microbial origin are imperative

Method used

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  • Application of compound Harziphilone
  • Application of compound Harziphilone
  • Application of compound Harziphilone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Embodiment 1: the preparation of compound Harziphilone

[0014] For the preparation of the compound Harziphilone shown in formula (1), see literature Jingfang Qian-Cutrone, Stella Huang, Li-Ping Chang, et al.Harziphilone and fleephilone, two new HIVREV / RRE binding inhibitors produced by Trichoderma harzianum.[J].Antibiotics , 1996, 49(10), 990-997.

Embodiment 2

[0015] Embodiment 2: insecticidal activity test

[0016] The insecticidal activity of the compounds represented by the formula (1) against Lygus spp. was determined by the test-tube drug film method (Wang Hongtao, Wang Lili, Liu Xueqing, Wang Yingzi. Indoor toxicity determination of 6 kinds of insecticides against 3rd instar nymphs of S. spp. Shandong Agricultural Science, 2014, 46(4), 92-94).

[0017] 1) Test insects

[0018] The overwintering eggs of Lygus viridatus in the winter jujube orchard were collected and reared with kidney beans in the greenhouse of Shandong Academy of Forestry Sciences. The next generation of nymphs were selected for testing when they reached the 3rd instar.

[0019] 2) Sample preparation

[0020] Take the sample to be tested (compound of formula (1)), dissolve it in acetone, mix thoroughly, and prepare a sample solution with a concentration of 200 μg / mL. Pipette 500 μL of the sample solution into another centrifuge tube, then add 500 μL of acet...

Embodiment 3

[0025] Embodiment 3: wettable powder

[0026] With reference to the above-mentioned documents, Trichoderma harzianum is fermented and cultivated, the fermented liquid is subjected to pressure filtration and precipitation, and the supernatant is concentrated so that the content of the compound shown in formula (1) is 1.5-2.0 wt%, to concentrate The quality of the supernatant is the base, add 25-35% white carbon black for adsorption, then add 3-8% sodium lauryl sulfate, 0.8-1.2% CMC and 2-4% pull-off powder, and then add attapulgite A wettable powder with a content of 0.5 wt% of the compound represented by formula (1) was prepared.

[0027] How to use: Spray 500 times liquid on Lygus ligus.

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Abstract

The invention discloses application of a compound Harziphilone. The structure of the compound is shown in a formula (I) and has better insecticidal activity for apolygus lucorum, the median lethal concentration (LC50) is 18.5 [mu]g / mL, and the compound can be taken as a novel pesticide ingredient with an insecticidal effect or a lead compound. The compound shown in the formula (I) is taken as a natural products derived from a microorganism, and is easy for large-scale fermentation preparation and easy to degrade in an environment.

Description

technical field [0001] The present invention relates to the use of insecticides of microbial origin, in particular the compound Harziphilone. Background technique [0002] The green bug Apolygus lucorum (Meyer-Dür) belongs to Hemiptera Miridae. In my country, it is mainly distributed in Hebei, Henan, Shandong and other Yellow River Basin areas. important pests. In recent years, with the adjustment of the agricultural industrial structure and the change of climatic conditions, Lygus aeruginosa has become the main pest that harms the winter jujube. Its adults and nymphs can eat the young leaves, flower buds and tender fruits of the winter jujube, seriously affecting the The output and quality of winter jujube cause serious economic losses. [0003] For a long time, chemical control has been the main method for the prevention and control of Lygus japonica in production, which can easily cause a series of problems such as pesticide residues in fruits, insect resistance and ecol...

Claims

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Application Information

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IPC IPC(8): A01N43/16A01P7/04
CPCA01N43/16
Inventor 牛赡光王清海段春华杜丰玉张淑静
Owner SHANDONG FOREST SCI RES INST
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