Detection method of glyphosate tolerance phenotype in transgenic maize based on chlorophyll fluorescence imaging
A technology of transgenic corn and chlorophyll fluorescence, which is applied in the direction of color/spectral characteristic measurement, fluorescence/phosphorescence, measuring devices, etc., can solve the problems of poor timeliness, unfavorable popularization and application, labor and material resources consumption, etc., and achieve easy operation, simple operation and environmental protection , to avoid the effect of cumbersome process
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[0038] Further detailed description will be given below in conjunction with the accompanying drawings and embodiments. The specific embodiments are implemented on the premise of the technical solutions of the present invention, and it should be understood that these modes are only used to illustrate the present invention and are not intended to limit the scope of the present invention. Such as figure 1 As shown, the present invention takes maize transfected with a bivalent gene (cry1Ab / cry2Aj-G10evo) as an example, and the phenotypes of other plants can be performed with reference to the method in this example.
[0039] 1. Prepare experimental samples. Plant 120 pots of transgenic (transformed into cry1Ab / cry2Aj-G10evo gene) and non-transgenic corn, and when the corn grows to the 3-leaf stage, the concentration is 1,080g a.e.ha -1 The glyphosate solution was placed in a portable CO2 high-pressure sprayer, and 80 transgenic and non-transgenic corn plants were sprayed as an ex...
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